scholarly journals Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

2003 ◽  
Vol 96 (3-4) ◽  
pp. 129-139 ◽  
Author(s):  
B.J.A Paule ◽  
V Azevedo ◽  
L.F Regis ◽  
R Carminati ◽  
C.R Bahia ◽  
...  
Keyword(s):  
Western Blotting ◽  
Primary Infection ◽  
Interferon Γ ◽  
Antigen Recognition ◽  
Corynebacterium Pseudotuberculosis ◽  
Igg Avidity ◽  
Kinetics Of

scholarly journals Pitfalls in the Serological Diagnosis of Primary Human Cytomegalovirus Infection in Pregnancy Due to Different Kinetics of IgM Clearance and IgG Avidity Index Maturation

Diagnostics ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 396
Author(s):  
Antonella Sarasini ◽  
Alessia Arossa ◽  
Maurizio Zavattoni ◽  
Chiara Fornara ◽  
Daniele Lilleri ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Human Cytomegalovirus ◽  
Primary Infection ◽  
Hcmv Infection ◽  
Serological Diagnosis ◽  
Avidity Index ◽  
Igm Antibody ◽  
Igg Avidity ◽  
Kinetics Of ◽  
In Pregnancy

Primary infection occurs when seronegative women are infected by human cytomegalovirus (HCMV). Diagnosis of primary infection is based on the following: antibody seroconversion, presence of IgM and low IgG avidity index (AI), and presence of DNAemia. The kinetics of HCMV-specific IgM antibody and maturation of AI might be very rapid or long-lasting during primary infection, which makes serological diagnosis insidious. The aims of this study were as follows: (i) to report atypical kinetics of HCMV-specific IgM antibody and AI early after onset of primary HCMV infection in a population of pregnant women, and (ii) to assess the frequency of such results. Altogether, 1309 sequential serum samples collected from 465 pregnant women with primary HCMV infection were included in the study. As a general rule, using the LIAISON®CMVIgMII and LIAISON®CMVIgGAvidityII assays, virus-specific IgM antibody levels decreased, while IgG AI increased over time during the first three months after infection onset. However, early clearance of IgM antibody and/or early IgG AI maturation occurred in 46/426 (10.7%) women. In more details, 20/426 (4.7%) and 26/418 (6.2%) women had undetectable IgM antibody or high IgG AI, respectively, when tested within 1–3 months after well-defined infection onset. Twenty sera from as many women with high IgG AI by the LIAISON assay were further tested for IgG AI by VIDAS®CMVIgGAvidityII and Mikrogen recomLineCMVIgG Avidity assays. Comparable results were obtained with VIDAS, whereas 14/20 sera gave low AI with the Mikrogen assay. In conclusion, about 11% of pregnant women undergoing a primary HCMV infection showed misleading serological results. Additional and appropriate testing might help in reducing the risk of missing HCMV primary infection in pregnancy. Furthermore, preconceptional testing should be strongly recommended.

scholarly journals Reconhecimento de antígenos por anticorpos de caprinos naturalmente infectados ou imunizados contra Corynebacterium pseudotuberculosis

2003 ◽  
Vol 2 (2) ◽  
Author(s):  
Vera Vale ◽  
Songeli Freire ◽  
Marcos Ribeiro ◽  
Lia Regis ◽  
Robson Bahia ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Western Blotting ◽  
Corynebacterium Pseudotuberculosis

<em>Corynebacterium pseudotuberculosis</em> é um cocobacilo gram-positivo, patógeno intracelular facultativo de macrófagos, filogeneticamente relacionado com o Mycobacterium tuberculosis. É amplamente distribuído em algumas espécies de animais, causando a linfadenite caseosa em ovinos e caprinos. A linfadenite caseosa, de ocorrência mundial, é uma doença infecciosa crônica cuja transmissão se dá principalmente através da pele. No presente experimento, foram estudados aspectos do reconhecimento antigênico, pela resposta humoral, em caprinos criados em regime extensivo, naturalmente infectados ou imunizados com uma vacina viva (cepa 1002), atenuada, desenvolvida pela Empresa Baiana de Desenvolvimento Agrícola (EBDA). As amostras de soros dos animais imunizados e de controle, coletadas mensalmente por um período de doze meses, foram analisadas pelo ensaio imunoenzimático ELISA e pelo Western blotting, utilizando-se como antígenos o extrato bacteriano contendo exotoxina e sonicado bacteriano de uma cepa selvagem e da cepa 1002. A análise através do Western blotting revelou que o padrão de antígenos reconhecidos pelos anticorpos séricos de animais imunizados, sem raça definida (srd) ou de duas raças puras, bem como animais srd naturalmente infectados, é semelhante, verificando-se bandas protéicas com pesos moleculares entre 20 e 94 kDa. Esta análise possibilitou observar-se que diferentes proteínas são reconhecidas ao longo da imunização.

Combination of line immunoassays Mikrogen recomLine CMV IgG and recomLine CMV IgG Avidity helps to date the onset of CMV primary infection

2019 ◽  
Vol 93 (3) ◽  
pp. 208-212
Author(s):  
Marie-Luce Delforge ◽  
Joëlle Eykmans ◽  
Deborah Steensels ◽  
Elena Costa ◽  
Catherine Donner ◽  
...  
Keyword(s):  
Primary Infection ◽  
Igg Avidity

Schistosoma mansoni: evidence that ‘non-permissiveness’ in 129/Ola mice involves worm relocation and attrition in the lungs

Parasitology ◽  
1989 ◽  
Vol 99 (3) ◽  
pp. 365-375 ◽  
Author(s):  
A. A. F. Elsaghier ◽  
P. M. Knopf ◽  
G. F. Mitchell ◽  
D. J. Mclaren
Keyword(s):  
Schistosoma Mansoni ◽  
Primary Infection ◽  
Histological Analysis ◽  
Liver Parenchyma ◽  
Pulmonary Vasculature ◽  
Inflammatory Reactions ◽  
Recovery Techniques ◽  
Worm Recovery ◽  
Worm Migration ◽  
Kinetics Of

Summary129/Ola mice resemble WEHI 129J mice in that around 70% of the individuals in any given population resist a primary infection withSchistosoma mansoni. Squashed-organ autoradiographic tracking of [Se]selenomethionine-labelled parasites has shown that the kinetics of worm migration in 129/Ola mice follows the expected pattern, and that all rodents harbour essentially similar numbers of worms on day 14 post-infection. Combined lung and liver worm recovery techniques have revealed, however, that segregation of mice into ‘permissive’ and ‘non-permissive’ individuals can first be detected on day 20. ‘Non-permissive’ mice are characterized by the absence of schistosome eggs at all times in the liver parenchyma and, in consequence, lack the attendant manifestations of pathology; they do, however, harbour a few stunted worms in the liver and significant numbers of adult schistosomes in the pulmonary vasculature. Histological analysis of sectioned lung tissue from such animals indicates that some lung-located schistosomes feed, pair and lay eggs. Nevertheless, eosinophil-enriched inflammatory reactions develop around such worms and the parasites themselves exhibit various manifestations of trauma, ranging from minor vacuolation to gut herniation and extrusion. The phenomenon of ‘non-permissiveness’ thus involves retardation of worm development in the liver and, in consequence, relocation of the parasites to the lungs, where they become subject to host effector responses.

scholarly journals Role of N-glycosylation in the synthesis, dimerization and secretion of human interferon-γ

1994 ◽  
Vol 303 (3) ◽  
pp. 831-840 ◽  
Author(s):  
T Sareneva ◽  
J Pirhonen ◽  
K Cantell ◽  
N Kalkkinen ◽  
I Julkunen
Keyword(s):  
Interferon Γ ◽  
Glycosylation Site ◽  
Biologically Active ◽  
Human Interferon ◽  
Mutant Form ◽  
Ifn Gamma ◽  
Glycosylation Sites ◽  
Mutant Forms ◽  
Kinetics Of

Human interferon-gamma (IFN-gamma) is a secretory glycoprotein, which has two potential N-linked glycosylation sites at positions Asn-25 and Asn-97 of its 143 amino acid long mature polypeptide chain. In order to understand the role of glycan residues in the synthesis and secretion of human IFN-gamma, both or either one of the potential N-linked glycosylation sites were mutated to Gln. The mutant and the wild-type (Wt) polypeptides were expressed in insect cells using a baculovirus vector. Elimination of the N-glycosylation site at position Asn-97 (N97Q) resulted in secreted protein yields of 70-90% as compared with the Wt production, whereas only 10-25% (N25Q) and 1-10% (N25Q,N97Q) levels of protein production was observed when the first or both sites were mutated, respectively. Although there was a difference between extracellular levels of produced protein, the kinetics of secretion was similar for all different IFN-gamma molecules. The Wt and the N-glycosylation site mutants were all secreted as dimers. The formation of biologically active dimers was more efficient for IFN-gamma polypeptides that had the intact glycosylation site at Asn-25 as compared with the other two mutant forms of IFN-gamma. The extent of dimerization correlated well with the observed secretion. The specific antiviral activity was of the same order (1 x 10(7) i.u./mg of protein) for the glycosylated IFN-gamma molecules, whereas it was slightly lower (0.5 x 10(7) i.u./mg of protein) for the unglycosylated mutant form.

scholarly journals Development of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros
Keyword(s):  
Immunoglobulin G ◽  
Rubella Virus ◽  
Primary Infection ◽  
Virus Infections ◽  
Serum Samples ◽  
Igg Avidity ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Semiautomated Method

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.

Sign in / Sign up

Export Citation Format

Share Document