Oral candidiasis in denture wearers — Anglogenic activity of blood mononuclear leukocytes

1997 ◽  
Vol 56 ◽  
pp. 144-145
Author(s):  
E. Mierzwińska ◽  
E. Skopińska ◽  
E. Sommer ◽  
E. Sikorska ◽  
E. Spiechowicz ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
Mononuclear Leukocytes

Effects of Retinoic Acid and 1,25-Dihydroxyvitamin D3 on IFN-gamma Secretion by Mononuclear Leukocytes from Nulliparous and Postparturient Dairy Cattle

2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz
Keyword(s):  
Retinoic Acid ◽  
Dairy Cattle ◽  
Dairy Cows ◽  
Dairy Cow ◽  
Pokeweed Mitogen ◽  
Mononuclear Leukocytes ◽  
Combined Effects ◽  
Ifn Gamma ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.

Leukocytes and Thrombosis

1965 ◽  
Vol 13 (01) ◽  
pp. 035-046 ◽  
Author(s):  
R. L Henry
Keyword(s):  
Tissue Culture ◽  
White Blood Cells ◽  
Polymorphonuclear Neutrophils ◽  
Mononuclear Leukocytes ◽  
Amoeboid Movement ◽  
Proliferating Cells ◽  
Cell Tissue ◽  
Blood Clots ◽  
Platelet Aggregates ◽  
To Come

SummaryWhite blood cells can no longer be considered simple trapped inclusions within thrombi. Their numbers in thrombi relative to blood counts increase with time. They appear to come from the blood flowing past the thrombus. They appear to migrate by amoeboid movement into the thrombic mass. Polymorphonuclear neutrophils have been shown to be lytic to fibrin and other proteins and thus can contribute to thrombus dissolution. There is increasing evidence that neutrophils may impart important cytotrophic function to proliferating cells during thrombus organization. Eosinophils are known to carr profibrinolysin and will release this precursor at sites of fibrin deposition. Mononuclear leukocytes can transform into fibroblasts in tissue culture and I consider a thrombus an ideal tissue culture medium. All of these cells can contribute to thrombus dissolution simply by mechanical weakening of the mass by migration into it, releasing enzymes, and allowing blood flow to carry away pieces of the thrombus as emboli. I extend my perspective on thrombosis by considering these intravascular solids as cell tissue cultures rather than simple blood clots or platelet aggregates.

Efek Proteksi Ekstrak Etanol Stichopus hermanii Terhadap Jumlah Limfosit pada Tikus yang Terpapar Asap Rokok dan Diinduksi Candida albicans

DENTA ◽  
2015 ◽  
Vol 9 (2) ◽  
pp. 146
Author(s):  
Auliasari Yunanda ◽  
Syamsulina Revianti ◽  
Isidora Karsini
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Control Group ◽  
Post Test

<p><strong><em>Latar Belakang: </em></strong>Merokok berhubungan dengan jamur rongga mulut yang dapat mengakibatkan <em>oral candidiasis</em>. <em>Stichopus hermanii</em><em> </em>mengandung efek antioksidan, antifungi dan immunostimulator. <strong><em>Tujuan: </em></strong>Mengevaluasi efek proteksi ekstrak <em>Stichopus hermanii </em>terhadap jumlah limfosit pada tikus Wistar yang terpapar asap rokok dan diinduksi <em>C.albicans.<strong> Bahan dan Metode: </strong></em>Rancangan penelitian ini adalah <em>post test-only control group</em> <em>design</em><strong><em>. </em></strong>42 ekor tikus Wistar jantan, dibagi menjadi 7 kelompok, Kelompok1 (saline 0,1mL, udara segar, CMC-Na 0,2%), Kelompok2 (saline 0,1mL, asap rokok, CMC-Na 0,2%), Kelompok3 (<em>C.albicans </em>0,1mL, udara segar, CMC-Na 0,2%), Kelompok4 (<em>C.albicans </em>0,1mL, asap rokok, CMC-Na 0,2%), Kelompok5 (saline 0,1mL, asap rokok, ekstrak <em>Stichopus hermanii</em> 0,02mg/kgBB), Kelompok6 (<em>C.albicans</em> 0,1mL, udara segar, ekstrak <em>Stichopus hermanii </em>0,02mg/kgBB), Kelompok7 (<em>C.albicans </em>0,1 mL, asap rokok, ekstrak <em>Stichopus hermanii </em>0,02mg/kgBB). Tikus Wistar diinduksi <em>C.albicans</em> 1 minggu, terpapar asap rokok 8 minggu, dan diberi ekstrak <em>Stichopus hermanii</em> 8 minggu. Selanjutnya, tikus Wistar dikorbankan setelah 2 bulan perlakuan. Jumlah limfosit dihitung melalui metode hapusan darah dengan <em>different counting</em> dibawah mikroskop cahaya dengan pembesaran 1000x. Data yang diperoleh dianalisis menggunakan uji <em>Kruskal-Wallis</em> dan <em>Mann-Whitney</em>.<strong><em> Hasil:</em></strong> Kelompok yang terpapar asap rokok dan diinduksi C.albicans memiliki dapat menurunkan jumlah limfosit, kelompok suplementasi menggunakan ekstrak ethanol <em>Stichopus hermanii</em> dapat meningkatkan jumlah limfosit<em>. </em><strong><em>S</em></strong><strong><em>impulan:</em></strong><strong> </strong>Suplementasi ekstrak <em>Stichopus hermanii</em> memiliki efek protektif untuk memicu proliferasi limfosit pada tikus Wistar setelah paparan asap rokok dan induksi <em>C.albicans</em>.</p>

Opportunistic infections among people living with HIV at reference health care centers in Morocco, 2015 (Preprint)

2019 ◽  
Author(s):  
Noureddine Sakhri ◽  
Fatima Zahra Meski ◽  
SOUMIA TRIKI
Keyword(s):  
Health Care ◽  
Opportunistic Infections ◽  
Oral Candidiasis ◽  
Clinical Stage ◽  
Bivariate Analysis ◽  
People Living With Hiv ◽  
Cross Sectional Survey ◽  
Pneumocystis Jiroveci ◽  
Cross Sectional ◽  
Health Care Centers

BACKGROUND Morbidity and mortality in HIV disease is due to immune-suppression leading to life-threatening opportunistic infections (OIs) during the natural course of the disease. In 2015, the HIV prevalence is low in general population and concentrated among key populations. OBJECTIVE This study aimed to assess the prevalence and CD4 correlates of OIs among adult HIV-infected patients attending antiretroviral health care in Morocco, during 2015. METHODS We conducted a cross-sectional survey among all adult PLHIV for admitted in the health care centers during 2015, who had acquired infection disease. Patients’ opportunistic infection status was determined through clinical diagnosis and laboratory investigations. CD4 count was determined using flow cytometry technique. The clinical stage of HIV was identified by the classification of Centers for Disease Control and Prevention (CDC). We collected Socio-demographic and clinical data from patients’ medical records. We performed statistical analysis by using Epi-Info 7.2.0.1 software. The appropriate test was applied, bivariate analysis was made and the differences were significant when p<.05. RESULTS 299 HIV-infected cases were included; 53% were males. The most represented age group was 25-34 years (36.1%). The mean age of the cases was 38.7 ± 16.8. The prevalence of OIs was 47.8%. Tuberculosis (65/299, 21.7%), Pneumocystis jiroveci pneumonia (40/299, 13.4%) and Oral candidiasis (22/299, 7.4%) were the most frequently observed OIs. CONCLUSIONS Tuberculosis, pneumocystis and oral candidacies were the leading OIs, encountered by HIV-infected cases. Preventive measures and early diagnosis of HIV associated to OIs are crucial.

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