EFEKTIVITAS PEMBERIAN EKSTRAK IKAN HARUAN (Channa striata) TERHADAP JUMLAH NEUTROFIL PADA PROSES PENYEMBUHAN ULKUS TRAUMATIKUS

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>

The Effect of Hyperbaric Oxygen Therapy to the Amount of Lymphocytes in Oral Candidiasis Immunosuppressed Model

<p><strong><em> </em></strong></p><p><strong><em>Background: </em></strong><em>Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. <strong>Purpose: </strong>To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. <strong>Methods:</strong> This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. <strong>Results:</strong> The data were analyzed with one way Anova test and showed significant difference among groups (p&lt;0,05). To show the difference between each groups we used LSD test and showed significant difference (p&lt;0,05) between K+ compared with P1, and K+ compared with K-. <strong>Conclusion:</strong> Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models.</em></p><p><em> </em><strong><em>Keywords:</em></strong><em> Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes</em></p><p> <strong><em>Correspondence</em></strong><strong><em>: </em></strong><em>Agni Febrina Pargaputri</em><em>, Departmen</em><em>t of</em><em> Oral </em><em>Biology </em><em>F</em><em>aculty of</em><em> </em><em>Dentistry</em><em>, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. </em><em>Phone:</em><em> 031-5945864, fax: 031-5912191, Email: </em><em><span style="text-decoration: underline;">agni.febrina</span></em><em><span style="text-decoration: underline;">@hangtuah.ac.id</span></em></p><p> </p>

Pengaruh ekstrak etanol daun ungu (EEDU) Graptophyllum pictum L. Griff terhadap aktivitas fagositosis monosit yang dipapar Candida albicans

<p class="Standard"><strong><em>Background</em></strong><em>:<strong> </strong>Candidiasis is one of oral infectious disease caused by Candida albicans.The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Grapthophyllum L. Griff has been proved to increase macrophage’s phagocytic activity, but the effect of violet leaves ethanol extract (EEDU) to increase monocyte’s phagocytic activity on C. albicans is unknown. <strong>Objectives</strong>:<strong> </strong>To determine the effect of violet leaves extract on monocyte’s phagocytic activity on C. albicans. <strong>Methods</strong>: This research was acted in vitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EEDU 2,5%, 5%, 10%. The percentage of activated monocytes was counted. <strong>Result</strong>: The result showed that incubation of EEDU 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p&lt;0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EEDU 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EEDU to increase monocyte’s phagocytic activity was 2,5%. <strong>Conclusion</strong>: The study showed that EEDU can increase monocyte’s phagocytic activity on C. albicans.</em></p><p class="Standard"><em> </em></p><p class="Standard"><strong><em>Keywords</em></strong><em>:Ethanol extract of Graptophyllum pictum L. Griff leaves, Monocyte’s Phagocytic Activity, Candida albicans </em></p>

Perawatan Ortodontik pada Kasus Distoversi Insisivus Sentral dan Transposisi Kaninus dan Insisivus Lateral Kanan Rahang Atas (Tahap Pertama)

<p><em><strong>Background:</strong> Tooth transposition is a rare and severe positional anomaly that may create many orthodontic problems from both esthetic and functional points of view. In this</em><em> case</em><em> report, the orthodontic management </em><em>of distoversion upper central incisor and</em><em> transposition of the upper right canine and lateral incisor. The patient was treated with a fixed appliance for 4 months. At this moment the central incisor</em><em> has</em><em> already corrected, </em><em>lateral incisor has also erupted and aligned. Patient management should be continued with phase two treatment to correct the transposition of canine</em><em>.</em></p><p><em><strong>Keywords</strong>: </em><em>Distoversion</em><em>, tooth transposition, orthodontic management.</em></p><p><em><strong>Correspondence :</strong> Departement of Pediatric Dentistry, College of Dentistry, University of Airlangga, Surabaya, East Java, Indonesia.</em></p><p><strong> </strong></p>

Effect of Stichopus Hermanni to Bigonial Width on Maxillary Suture Expansion using Cephalometric Analysis

<p><strong><em>Background:</em></strong><em>Maxillary expansion uses mechanical forces to open the maxillary suture which causes inflammation and bone remodeling around the area. Stichopus hermanni is known to contain flavonoid, chondroitin sulfate, and saponin which is used to inhibit inflammatory process, increase bone metabolism and mineralization, and wound healing. <strong>Purpose:</strong> The aim of this experiment is to know the effect of Stichopus hermanni to bigonial width on maxillary suture remodeling expansion using cephalometric analysis. </em><strong><em>Materials and </em></strong><strong><em>Methods: </em></strong><em>Experimental units used were 24 male Cavia cobaya divided into 4 groups (n=6). Group K(-) is negative control. Helical spring was applied and activated in the other 3 groups for 10 days. Group K(+) was given 2% NaCMC gel, both group P1 and P2 was given 3% Stichopus hermanni gel. Group P2 were also given retention period for 10 days after activation period. Cavia cobaya were then decapitated and observed for the bigonial width changes. The data was analyzed with One Way ANOVA test continued with LSD test. <strong>Result</strong></em><strong><em>s</em></strong><strong><em>:</em></strong><em> The result of ANOVA test showed siginficant differences among the treatment groups p=0,000 (p&lt;0,05). LSD test showed significant differences between group K(-) and K(+) (Sig. 0,005), K(-) and P1 (Sig. 0,001), and group K(-) and P2 (Sig. 0,000). <strong>Conclusion: </strong>3% Stichopus hermanni gel increases bigonial width but not significant in remodeling process on maxillary suture expansion.</em></p><p><em> </em></p><p><strong><em>Keywords: </em></strong><em>Stichopus hermanni, bone remodeling, maxillary suture expansion, bigonial width, cephalometric analysis</em></p><p><strong><em> </em></strong></p><p><strong><em>Correspondence: </em></strong><em>Noengki Prameswari, Department of Orthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, 5912191, Email: </em><a href="mailto:[email protected]"><em>[email protected]</em></a><em></em></p>

Study of Hyperbaric Oxygen Theraphy and Gold sea Cucumber (Stichopus Hermanii) 3% for Collagen Density of Rat’s with Diabetes Mellitus Induced by Porphyromonas Gingivalis Bactery

<p><strong><em>Background:</em></strong><em> Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM</em><em>. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. <strong>Purpose:</strong> To determine the therapeutic effects of HBO 2,4 ATA 3x3’ interval 5’ 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. <strong>Materials and Methods:</strong></em><em>The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis </em><em>1x10⁹sel/ml for 5 days straight to make them suffer periodontitis</em><em>,</em><em> 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. <strong>Results:</strong> Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly</em> K1<em>(1 ± 0.00)</em> - K2<em>(2±0.00)</em>, dan K1<em>(1 ± 0.00)</em> - K4<em>(2.5 ± 0.289)</em><em>.<strong>Conclution</strong></em><strong><em>:</em></strong><em>Therapy HBO combination of 2.4 ATA 3 x 30 </em><em>with 5 minutes air breaks for 7 days</em><em> and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM. <br /> <br /> </em></p><p><strong><em>Keywords:</em></strong><em>OHB, Stichopus hermanii, periodontitis with DM, collagen</em></p><p><strong><em> </em></strong></p><strong><em>Correspondence: </em></strong><em>Dian Mulawarmanti, Department of Oral Biology, Faculty of Dentistry, Hang Tuah University,Arif Rahman Hakim 150, Surabaya,Phone 031-5945864, Email: </em><a href="mailto:[email protected]"><em>[email protected]</em></a>

Effectiveness of Chitosan Solution as Denture Cleanser to Inhibit the Growth of Candida albicans on Acrylic, Valplast and Luciton-FRS

<p><strong><em>Background: </em></strong><em>Heat cure acrylic and nylon thermoplastic valplast and lucitone-FRS brands are the three materials most often used as denture bases. Candida albicans is the dominant microorganism that can cause denture stomatitis. Cleaning denture can be done in two ways, namely the mechanical way with a toothbrush or an ultrasonic cleanser and a chemical method by immersing the denture into the cleaning solution. Chitosan solution is antibacterial and anti fungal which can inhibit the growth of Candida albicans colonies on heat cured acrylic plates, valplast and lucitone-FRS. <strong>Purpose:</strong> To determine the concentration of chitosan solution and the type of denture plate that most effectively inhibited the growth of Streptococcus mutans Candida albicans colonies on heat cured acrylic, valplast and lucitone-FRS plates. <strong>Materials and Methods: </strong>21 heat cured acrylic plate samples, 21 valplast plate samples, 21 lucitone-FRS plate samples measuring 10x10x2 mm divided by 9 groups. Heat cured, valplast and lucitone-FRS acrylic plate samples were contaminated with Candida albicans then immersed using the concentration of 0.25%, 0.5% chitosan solution and sterile aquades as the control group with 90 minutes immersion time. The entire study sample was calculated using Candida albicans colonies on Sabourroud's Dextrose Agar media. <strong>Results: </strong>The Kruskall Wallis test showed a significant difference (p &lt;0.05) in all treatment groups. Mann-Whitney showed a significant difference (p &lt;0.05) in all groups in the Candida albicans study. <strong>Conclusion: </strong>Soaking the lucitone-FRS plate in 0.5% chitosan solution for 90 minutes was the most effective in inhibiting the growth of Candida albicans colonies.</em></p><p><strong> </strong></p><p><strong><em>Keywords:</em></strong><em>Kitosan, Candida albicans, akrilik heat cured, valplast, lucitone-FRS</em></p><p><strong><em> </em></strong></p><p><strong><em>Correspondence: </em></strong><em>Anindita Apsari, </em><em>Department of Prosthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya</em><em>, Email : </em><a href="mailto:[email protected]"><em>[email protected]</em></a><em>/</em><a href="mailto:[email protected]"><em>[email protected]</em></a><em></em></p>