GST fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies

2001 ◽  
Vol 91 (2) ◽  
pp. 139-147 ◽  
Author(s):  
Mei-Yun Zhang ◽  
Stefan Schillberg ◽  
Sabine Zimmermann ◽  
Yu-Cai Liao ◽  
Gudrun Breuer ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Movement Protein ◽  
False Positives ◽  
Single Chain ◽  
Viral Movement Protein ◽  
Single Chain Antibodies ◽  
Viral Movement ◽  
Selection Of ◽  
Gst Fusion Proteins

scholarly journals Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies using Cell-Free Ribosome Display

2018 ◽  
Author(s):  
Adinarayana Kunamneni ◽  
Elizabeth C. Clarke ◽  
Chunyan Ye ◽  
Steven B. Bradfute ◽  
Ravi Durvasula
Keyword(s):  
Hemorrhagic Fever ◽  
Cross Reactivity ◽  
Free Ribosome ◽  
Rapid Diagnostics ◽  
Single Chain ◽  
Ribosome Display ◽  
Single Chain Antibodies ◽  
Single Chain Fv ◽  
New Generation ◽  
Selection Of

AbstractFiloviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there are a lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.

scholarly journals Plasmodesma-Mediated Selective Protein Traffic between “Symplasmically Isolated” Cells Probed by a Viral Movement Protein

2002 ◽  
Vol 14 (9) ◽  
pp. 2071-2083 ◽  
Author(s):  
Asuka Itaya ◽  
Fengshan Ma ◽  
Yijun Qi ◽  
Yoshie Matsuda ◽  
Yali Zhu ◽  
...  
Keyword(s):  
Movement Protein ◽  
Isolated Cells ◽  
Viral Movement Protein ◽  
Protein Traffic ◽  
Viral Movement

scholarly journals Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

2004 ◽  
Vol 70 (11) ◽  
pp. 6936-6939 ◽  
Author(s):  
Vicente Monedero ◽  
Jes�s Rodr�guez-D�az ◽  
Rosa Viana ◽  
Javier Buesa ◽  
Gaspar P�rez-Mart�nez
Keyword(s):  
Lactobacillus Casei ◽  
Probiotic Bacteria ◽  
Extracellular Expression ◽  
Outer Capsid Protein ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Outer Capsid ◽  
Selection Of

ABSTRACT Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

In vivo selection of single-chain antibodies using a yeast two-hybrid system

2000 ◽  
Vol 238 (1-2) ◽  
pp. 161-172 ◽  
Author(s):  
Antje Pörtner-Taliana ◽  
Marijane Russell ◽  
Karen J Froning ◽  
Paul R Budworth ◽  
John D Comiskey ◽  
...  
Keyword(s):  
Hybrid System ◽  
Single Chain ◽  
Yeast Two Hybrid ◽  
Single Chain Antibodies ◽  
In Vivo Selection ◽  
Yeast Two Hybrid System ◽  
Two Hybrid ◽  
Selection Of
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