Immunochemiluminescent focus assays for the quantitation of hepatitis A virus and rotavirus in cell cultures

2001 ◽  
Vol 94 (1-2) ◽  
pp. 69-80 ◽  
Author(s):  
Gary P Richards ◽  
Michael A Watson
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Detection of Hepatitis a Virus and other Enteroviruses in Wastewater and Surface Water Samples by Gene Probe Assay

1991 ◽  
Vol 24 (2) ◽  
pp. 267-272 ◽  
Author(s):  
S. Dubrou ◽  
H. Kopecka ◽  
J. M. Lopez Pila ◽  
J. Maréchal ◽  
J. Prévot
Keyword(s):  
Surface Water ◽  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Limit Of Detection ◽  
Blot Hybridization ◽  
Noncoding Region ◽  
Gene Probe ◽  
Dot Blot ◽  
Lower Efficiency

Enteroviruses were specifically detected by dot blot hybridization when using poliovirus type 1 (PV1) derived subgenomic radiolabeled cRNA probes (riboprobes) in environmental water specimens and in the cell cultures in which the viruses were amplificated. The riboprobe corresponding to the 5' noncoding sequence detected the majority of enteroviruses. Hepatitis A virus (HAV) was specifically detected by an HAV cRNA probe corresponding to the 5' noncoding region of its genome. By this test, the limit of detection of coxsackievirus B5 and echovirus 7 seeded in mineral water was 103 to 104 PFU/spot. In cell cultures, positive signals were observed in the lysates of cells infected by one PFU. Higher positive signals were obtained with a short PV1 probe (nt 221-670) corresponding to the 5' noncoding region, which is a well preserved sequence among the enteroviruses, than with PV1 genomic probe. Hybridization allowed a good detection of enteroviral RNAs in wastewater specimens, but with a lower efficiency in surface water. In this case, amplification of viruses in the cell cultures gave significant hybridization results.

scholarly journals In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

1994 ◽  
Vol 60 (6) ◽  
pp. 1921-1926 ◽  
Author(s):  
J L Romalde ◽  
M K Estes ◽  
G Szücs ◽  
R L Atmar ◽  
C M Woodley ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
In Situ Detection

Preparation of a Prototype Inactivated Hepatitis A Virus Vaccine from Infected Cell Cultures

1986 ◽  
Vol 153 (4) ◽  
pp. 749-756 ◽  
Author(s):  
L. N. Binn ◽  
W. H. Bancroft ◽  
S. M. Lemon ◽  
R. H. Marchwicki ◽  
J. W. LeDuc ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Cultures ◽  
Virus Vaccine ◽  
Hepatitis A ◽  
Hepatitis A Virus

Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures.

1984 ◽  
Vol 20 (1) ◽  
pp. 28-33 ◽  
Author(s):  
L N Binn ◽  
S M Lemon ◽  
R H Marchwicki ◽  
R R Redfield ◽  
N L Gates ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Serial Passage ◽  
Primary Isolation ◽  
Primate Cell ◽  
Virus Strains

Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures.

1983 ◽  
Vol 17 (5) ◽  
pp. 834-839 ◽  
Author(s):  
S M Lemon ◽  
L N Binn ◽  
R H Marchwicki
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus

Continuous production of hepatitis A virus in PLC/PRF/5 cell cultures: use of antigen for serology

1987 ◽  
Vol 18 (2-3) ◽  
pp. 193-203 ◽  
Author(s):  
J.M. Crance ◽  
J. Passagot ◽  
E. Biziagos ◽  
R. Deloince
Keyword(s):  
Cell Cultures ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Continuous Production

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

Hepatitis A Antigen in Liver, Bile and Stool

1975 ◽  
Vol 33 ◽  
pp. 340-341
Author(s):  
D.R. Jackson ◽  
J.H. Hoofnagle ◽  
A.N. Schulman ◽  
J.L. Dienstag ◽  
R.H. Purcell ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Liver Enzymes ◽  
Hepatitis A Virus ◽  
Type A ◽  
Initial Study ◽  
Virus Like Particle ◽  
Elevated Liver Enzymes ◽  
Ag Particles ◽  
Ag Particle ◽  
Human Stool

Using immune electron microscopy Feinstone et. al. demonstrated the presence of a 27 nm virus-like particle in acute-phase stools of patients with viral hepatitis, type A, These hepatitis A antigen (HA Ag) particles were aggregated by convalescent serum from patients with type A hepatitis but not by pre-infection serum. Subsequently Dienstag et. al. and Maynard et. al. produced acute hepatitis in chimpanzees by inoculation with human stool containing HA Ag. During the early acute disease, virus like particles antigenically, morphologically and biophysically identical to the human HA Ag particle were found in chimpanzee stool. Recently Hilleman et. al. have described similar particles in liver and serum of marmosets infected with hepatitis A virus (HAV). We have investigated liver, bile and stool from chimpanzees and marmosets experimentally infected with HAV. In an initial study, a chimpanzee (no.785) inoculated with HA Ag-containing stool developed elevated liver enzymes 21 days after exposure.

Sign in / Sign up

Export Citation Format

Share Document