Detection of Neospora caninum and Toxoplasma gondii DNA by single tube nested polymerase chain reaction

1997 ◽  
Vol 30 (3) ◽  
pp. 237 ◽  
Author(s):  
J. Ellis ◽  
D. McMillan ◽  
D. Croan ◽  
L. Reddacliff ◽  
P.A.W. Harper
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Single Tube ◽  
Polymerase Chain

scholarly journals Detection of NaturalToxoplasma gondiiInfection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
John Mokua Mose ◽  
John Maina Kagira ◽  
Simon Muturi Karanja ◽  
Maina Ngotho ◽  
David Muchina Kamau ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Human Beings ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Free Range ◽  
Age Dependent ◽  
Polymerase Chain ◽  
The Difference

The detection ofToxoplasma gondiiin free-range chickens is a good indicator of possible risk to human beings. The aim of this study was to investigate the occurrence ofT. gondiiin free-range chicken using polymerase chain reaction (PCR). Brain samples from 105 free-range chickens from three administrative areas in Thika region, Kenya, were collected, DNA-extracted, and analyzed using PCR to detect presence ofT. gondii. The overall prevalence ofT. gondiiin all the three areas was 79.0% (95% CI: 70.0–86.4%) and the prevalence across the three areas was not significantly different (P=0.5088;χ2=1.354). Female chickens had higher (79.4%) prevalence than males (78.6%), although the difference was not significant (P=0.922,χ2= 0.01). However, chickens that were more than 2 years old had significantly (P=0.003;χ2= 11.87) higher prevalence compared to younger ones. The study indicates that there was a high occurrence ofT. gondiiinfection in free-range chickens from Thika region and that the infection rate is age dependent. Further studies should be carried out to determine the possible role of roaming chickens in the epidemiology of the disease among humans in the area.

Cutaneous Toxoplasmosis in a Female Japanese Cat

2007 ◽  
Vol 44 (5) ◽  
pp. 683-687 ◽  
Author(s):  
C.-H. Park ◽  
H. Ikadai ◽  
E. Yoshida ◽  
H. Isomura ◽  
H. Inukai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Polymerase Chain Reaction ◽  
Endothelial Cells ◽  
Mammary Gland ◽  
Sequence Analysis ◽  
Toxoplasma Gondii ◽  
Deoxyribonucleic Acid ◽  
Neospora Caninum ◽  
Chain Reaction ◽  
Polymerase Chain

A 16-year-old female Japanese cat was presented with a single mammary-gland nodule approximately 3 cm in diameter. Histologically, the nodule consisted of necrotizing granulomatous panniculitis, vasculitis, and mastitis, and contained free and clustered protozoal organisms. The organism was present in the cytoplasm of macrophages, fibroblasts, endothelial cells, and mammary-gland epithelia. The organism was positive for anti- Toxoplasma gondii and anti- Neospora caninum antibodies. Electron microscopy showed single and grouped tachyzoites, with morphologic features similar to those of T. gondii. Polymerase chain reaction and deoxyribonucleic acid sequence analysis was consistent with T. gondii infection. This is the first report of cutaneous toxoplasmosis in a Japanese cat.

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