384 GENES IN WIDE FUNCTION MORE THAN IMMUNE RESPONSE WERE INVOLVED IN CHRONIC HBV INFECTION BY ChIP-CHIP ANALYSIS OF H3k9 ACETYLATION STATUS IN CD4+ T CELLS

Abstract Background: The restoration of host hepatitis B virus (HBV)-specific antiviral immunity is an effective strategy for hepatitis B recovery. Follicular dendritic cells (FDCs) play a crucial role in immune regulation. The goal of the present study was to investigate the characteristics and functions of FDCs in chronic HBV infection. Methods: The frequencies of FDCs in peripheral blood, liver, and spleen were measured in patients with chronic HBV infection. Isolated FDCs from splenic tissues of HBV-related liver cirrhosis-induced hypersplenism patients were cultured with autologous intrasplenic CD4 + T cells and CD19 + B cells.Results: We found that patients with chronic HBV infection had a significantly increased frequency of circulating FDCs compared with that of healthy controls. Additionally, the frequency of circulating FDCs was positively correlated with that of intrahepatic and intrasplenic counterparts. Moreover, a positive correlation between the frequency of circulating FDCs and plasmablast and memory B cells, as well as C-X-C motif chemokine receptor type 5 (CXCR5) + CD4 + T cells and CXCR5 + CD8 + T cells was also observed. Notably, in vitro experiments demonstrated that FDCs derived from splenic tissues of chronic HBV patients facilitated interferon-γ and interleukin-21 production from autologous intrasplenic CD4 + T cells and promoted the proliferation of autologous intrasplenic CD19 + B cells. Conclusions: Expanded FDCs in patients with chronic HBV infection may favor the host immune responses against HBV. The identification of this unique population may contribute to a better understanding of the immune regulatory mechanisms and provide a potential immunotherapeutic target in chronic HBV infection.

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TNF-α/IFN-γ profile of HBV-specific CD4 T cells is associated with liver damage and viral clearance in chronic HBV infection

Journal of Hepatology ◽

10.1016/j.jhep.2019.08.024 ◽

2020 ◽

Vol 72 (1) ◽

pp. 45-56 ◽

Cited By ~ 13

Author(s):

Haoliang Wang ◽

Heng Luo ◽

Xing Wan ◽

Xiaolan Fu ◽

Qing Mao ◽

...

Keyword(s):

T Cells ◽

Liver Damage ◽

Cd4 T Cells ◽

Viral Clearance ◽

Hbv Infection ◽

Chronic Hbv Infection ◽

Tnf Α ◽

Ifn Γ ◽

Chronic Hbv

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TIGIT on Exhausted T Cells Serves as a Target for Immune Restoration in Patients with Chronic HBV Infection

SSRN Electronic Journal ◽

10.2139/ssrn.3276408 ◽

2018 ◽

Author(s):

Yanyan Wei ◽

Jing Fan ◽

Dandan Yin ◽

Lili Wang ◽

Wei Zhao ◽

...

Keyword(s):

T Cells ◽

Hbv Infection ◽

Immune Restoration ◽

Chronic Hbv Infection ◽

Chronic Hbv

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Restoration of a functional antiviral immune response to chronic HBV infection by reducing viral antigen load: If not sufficient, is it necessary?

Emerging Microbes & Infections ◽

10.1080/22221751.2021.1952851 ◽

2021 ◽

pp. 1-23

Author(s):

Sisi Yang ◽

Wanjia Zeng ◽

Jiming Zhang ◽

Fengmin Lu ◽

Jinhong Chang ◽

...

Keyword(s):

Immune Response ◽

Viral Antigen ◽

Hbv Infection ◽

Antiviral Immune Response ◽

Chronic Hbv Infection ◽

Chronic Hbv

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CXCL13-mediated recruitment of intrahepatic CXCR5+CD8+ T cells favors viral control in chronic HBV infection

Journal of Hepatology ◽

10.1016/j.jhep.2019.09.031 ◽

2020 ◽

Vol 72 (3) ◽

pp. 420-430 ◽

Cited By ~ 7

Author(s):

Yongyin Li ◽

Libo Tang ◽

Ling Guo ◽

Chengcong Chen ◽

Shuqin Gu ◽

...

Keyword(s):

T Cells ◽

Cd8 T Cells ◽

Hbv Infection ◽

Viral Control ◽

Chronic Hbv Infection ◽

Chronic Hbv

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Increased CCR7loPD-1hiCXCR5+CD4+ T Cells in Peripheral Blood Mononuclear Cells Are Correlated with Immune Activation in Patients with Chronic HBV Infection

Canadian Journal of Gastroenterology and Hepatology ◽

10.1155/2018/1020925 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9

Author(s):

Ya-Xin Huang ◽

Qi-Yi Zhao ◽

Li-Li Wu ◽

Dong-Ying Xie ◽

Zhi-Liang Gao ◽

...

Keyword(s):

T Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Antiviral Treatment ◽

Hbv Infection ◽

Peripheral Blood Mononuclear ◽

Chronic Hbv Infection ◽

Chronic Hepatitis B Virus ◽

Chronic Hbv ◽

Immune Tolerant

T follicular helper cells (Tfh cells) affect essential immune pathogenesis in chronic hepatitis B virus (HBV) infection. The CCR7loPD-1hi Tfh subset has a partial Tfh effector phenotype and is associated with active Tfh differentiation, whereas the CCR7hiPD-1lo Tfh subset is a resting phenotype. We recruited 20 healthy volunteers and 77 patients with chronic HBV infection, including those in the immune tolerant (IT) phase (n=19), immune clearance (IC) phase (n=20), low replicative (LR) phase (n=18), and reactivation (RA) phase (n=20). The expression of CD4, CXCR5, PD-1, and CCR7 was detected in T cells from peripheral blood by flow cytometry. The frequency of the CCR7loPD-1hi T subset was significantly higher in the patients than in the healthy controls (14.92±4.87% vs 12.23±2.95%, p=0.018). The frequency of this Tfh subset in the IC group (18.42%±3.08) was increased compared with the IT group (11.94±2.87%, p=0.001) and LR group (13.65±4.93%, p=0.031) and was higher in the RA group than in the IT group (16.03±5.37% vs 11.94±2.87%, p=0.030). We observed a weak positive correlation between the CCR7loPD-1hi Tfh subset population and the alanine transaminase (ALT) level (r=0.370, p=0.001). The CCR7loPD-1h Tfh subset in the chronic HBV-infected patients was elevated to various degrees among the different immune phases. CCR7loPD-1hiCXCR5+CD4+ T cells are correlated with the immune status of chronic HBV infection patients and may be developed as a potential indicator for antiviral treatment.

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Recombinant hepatitis B core antigen carrying preS1 epitopes induce immune response against chronic HBV infection

Vaccine ◽

10.1016/j.vaccine.2003.07.014 ◽

2004 ◽

Vol 22 (3-4) ◽

pp. 439-446 ◽

Cited By ~ 31

Author(s):

Xinchun Chen ◽

Meizhong Li ◽

Xiaohua Le ◽

Weimin Ma ◽

Boping Zhou

Keyword(s):

Immune Response ◽

Hepatitis B ◽

Hbv Infection ◽

Core Antigen ◽

Recombinant Hepatitis ◽

Chronic Hbv Infection ◽

Chronic Hbv ◽

Hepatitis B Core Antigen

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Hepatitis B vaccination status and vaccine immune response among children in rural Senegal

European Journal of Public Health ◽

10.1093/eurpub/ckz185.592 ◽

2019 ◽

Vol 29 (Supplement_4) ◽

Author(s):

L Perieres ◽

M Coste ◽

S Ndiour ◽

P Halfon ◽

C Sokhna ◽

...

Keyword(s):

Immune Response ◽

Hepatitis B ◽

Chronic Infection ◽

Vaccination Status ◽

Hbv Infection ◽

Hepatitis B Vaccination ◽

Chronic Hbv Infection ◽

Hbv Vaccination ◽

Chronic Hbv ◽

Vaccine Immunity

Abstract Background Hepatitis B vaccination during childhood is key to reduce the prevalence of Hepatitis B virus (HBV) infection. In Senegal, a highly endemic country, the three-dose hepatitis B vaccine and the birth dose vaccine were introduced in the Expanded Programme on Immunization (EPI) in 2004 and 2016 respectively. This study aimed to determine chronic HBV infection prevalence, hepatitis B vaccination status and vaccine immunity among children in Senegal. Methods A cross-sectional study including HBV screening was conducted at home among children aged 6 months to 15 years (i.e. born after the introduction of the HBV vaccine in the EPI) in the rural zone of Niakhar. Dried Blood Spot (DBS) samples were collected for the detection of HBsAg, anti-HBc Ab and anti-HBs Ab using chemoluminescence. Vaccination status was assessed using information on vaccination cards. Detectable vaccine immunity was defined with an adjusted DBS threshold of DOI≥0.36 IU/mL (corresponding to 10 IU/mL in venous blood sampling). Results Between October and December 2018, 455 children were enrolled. Preliminary results show that 7/455 (1.5%) had been in contact with HBV (positive anti-HBc Ab) and 5/455 (1.1%) had chronic HBV infection (positive HBsAg). Only 161/455 (35.4%) children had a vaccination card available. Among those, 150/161 (93.2%) received at least 3 doses of hepatitis B vaccine, of which 83/150 (55.3%) had detectable vaccine immunity. The proportion of children with detectable vaccine immunity was significantly higher in children <5 years than in children aged 5-9 and 10-15 (72.3% versus 47.3%, p = 0.006 and 72.3% versus 14.3%, p < 0.001). Conclusions Preliminary results suggest a low prevalence of HBV chronic infection among children born after the introduction of HBV vaccination in Senegal. However, detectable vaccine immunity rapidly decreases with age among vaccinated children, signalling a need for further studies on the immune response to HBV vaccination in this context. Key messages HBV chronic infection is low among children born after the introduction of HBV vaccination in Senegal. Further studies on the immune response to HBV vaccination in this context are needed.

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