Therapeutic efficacy of hepatitis B surface antigen–antibodies-recombinant DNA composite in HBsAg transgenic mice

Vaccine ◽  
2001 ◽  
Vol 19 (30) ◽  
pp. 4219-4225 ◽  
Author(s):  
Bo-Jian Zheng ◽  
Mun-Hon Ng ◽  
Li-Fang He ◽  
Xin Yao ◽  
Kwok-Wah Chan ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Therapeutic Efficacy ◽  
Recombinant Dna ◽  
Hepatitis B Surface Antigen

scholarly journals Hepatitis B surface antigen gene expression is regulated by sex steroids and glucocorticoids in transgenic mice.

1987 ◽  
Vol 84 (5) ◽  
pp. 1187-1191 ◽  
Author(s):  
H. Farza ◽  
A. M. Salmon ◽  
M. Hadchouel ◽  
J. L. Moreau ◽  
C. Babinet ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Sex Steroids ◽  
Hepatitis B Surface Antigen ◽  
Antigen Gene

scholarly journals Expression of hepatitis B virus large envelope polypeptide inhibits hepatitis B surface antigen secretion in transgenic mice.

1986 ◽  
Vol 60 (3) ◽  
pp. 880-887 ◽  
Author(s):  
F V Chisari ◽  
P Filippi ◽  
A McLachlan ◽  
D R Milich ◽  
M Riggs ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
B Virus

scholarly journals Breaking tolerance in hepatitis B surface antigen (HBsAg) transgenic mice by vaccination with cross-reactive, natural HBsAg variants

2003 ◽  
Vol 33 (12) ◽  
pp. 3342-3352 ◽  
Author(s):  
Reinhold Schirmbeck ◽  
Nektarios Dikopoulos ◽  
Marcin Kwissa ◽  
Frank Leithäuser ◽  
Kasper Lamberth ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen

scholarly journals CpG Oligodeoxynucleotides with Hepatitis B Surface Antigen (HBsAg) for Vaccination in HBsAg-Transgenic Mice

2001 ◽  
Vol 75 (14) ◽  
pp. 6482-6491 ◽  
Author(s):  
E. Malanchère-Brès ◽  
P. J. Payette ◽  
M. Mancini ◽  
P. Tiollais ◽  
H. L. Davis ◽  
...  
Keyword(s):  
T Cells ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Transgene Expression ◽  
Hepatitis B Surface Antigen ◽  
Cpg Oligodeoxynucleotides ◽  
Enhanced Production ◽  
B Virus

ABSTRACT DNA motifs containing unmethylated CpG dinucleotides within the context of certain flanking sequences enhance both innate and antigen-specific immune responses, due in part to the enhanced production of Th1-type cytokines. Here we explored the ability of CpG-containing oligodeoxynucleotides combined with recombinant hepatitis B surface antigen (HBsAg) to induce Th1 responses in mice that are transgenic for this antigen and that represent a model for asymptomatic hepatitis B virus chronic carriers. This was compared to hepatitis B virus-specific DNA-mediated immunization, which we have previously shown to induce the clearance of the transgene expression product and the down-regulation of hepatitis B virus mRNA in this transgenic mouse lineage. In control nontransgenic C57BL/6 mice, three immunizations with HBsAg and CpG triggered the production of anti-HBs antibodies and of HBs-specific T cells that secrete gamma interferon but do not display any HBsAg-specific cytotoxic activity. In the HBsAg-transgenic mice, immunization with HBsAg and CpG oligodeoxynucleotides, but not with CpG alone, induced the clearance of HBsAg circulating in the sera, with a concomitant appearance of specific antibodies, and was able to regulate the hepatitis B virus mRNA constitutively expressed in the liver. Finally, adoptive transfer experiments with CD8+ T cells primed in C57BL/6 mice with HBsAg and CpG oligodeoxynucleotide-based immunization show that these cells were able to partially control transgene expression in the liver and to clear the HBsAg from the sera of recipient transgenic mice without an antibody requirement. CpG oligodeoxynucleotides motifs combined with HBsAg could therefore represent a potential therapeutic approach with which to treat chronically infected patients.

scholarly journals Dynamic of Immune Response induced in Hepatitis B Surface Antigen-transgenic Mice Immunized with a Novel Therapeutic Formulation

2016 ◽  
Vol 6 (1) ◽  
pp. 25-30 ◽  
Author(s):  
JC Aguilar ◽  
Freya M Freyre Almeida ◽  
Aracelys Blanco ◽  
Heidy Trujillo ◽  
Dunia Hernández ◽  
...  
Keyword(s):  
Immune Response ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Novel Therapeutic
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