A peptide construct containing B-cell and T-cell epitopes from the foot-and-mouth disease viral VP1 protein induces efficient antiviral protection

Vaccine ◽  
1999 ◽  
Vol 17 (6) ◽  
pp. 577-584 ◽  
Author(s):  
O.M Volpina ◽  
A.Yu Surovoy ◽  
M.N Zhmak ◽  
M.A Kuprianova ◽  
D.O Koroev ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Vp1 Protein ◽  
Foot And Mouth

scholarly journals B Epitope Multiplicity and B/T Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Esther Blanco ◽  
Carolina Cubillos ◽  
Noelia Moreno ◽  
Juan Bárcena ◽  
Beatriz G. de la Torre ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Neutralizing Antibodies ◽  
Cell Epitope ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitope ◽  
T Cell Epitopes ◽  
B Cell Epitope ◽  
Foot And Mouth

Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) elicit potent B- and T-cell specific responses and confer protection to viral challenge, while juxtaposition of these epitopes in a linear peptide induces less efficient responses. To assess the relevance of B-cell epitope multivalency, dendrimers bearing two (B2T) or four (B4T) copies of the B-cell epitope from type O FMDV (a widespread circulating serotype) were tested in CD1 mice and showed that multivalency is advantageous over simple B-T-epitope juxtaposition, resulting in efficient induction of neutralizing antibodies and optimal release of IFNγ. Interestingly, the bivalent B2T construction elicited similar or even better B- and T-cell specific responses than tetravalent B4T. In addition, the presence of the T-cell epitope and its orientation were shown to be critical for the immunogenicity of the linear juxtaposed monovalent peptides analyzed in parallel. Taken together, our results provide useful insights for a more accurate design of FMD subunit vaccines.

scholarly journals Immunogenicity of Foot-and-Mouth Disease Virus Dendrimer Peptides: Need for a T-Cell Epitope and Ability to Elicit Heterotypic Responses

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4714
Author(s):  
Rodrigo Cañas-Arranz ◽  
Patricia de León ◽  
Sira Defaus ◽  
Elisa Torres ◽  
Mar Forner ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Epitope ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitope ◽  
T Cell Epitopes ◽  
Vaccine Platform ◽  
Mouth Disease Virus ◽  
Foot And Mouth

An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as a foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies of the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140–158)) covalently linked to a heterotypic T-cell epitope from non-structural protein 3A (21–35), henceforth B2T-3A, has previously been shown to elicit high neutralizing antibody (nAb) titers and IFN-γ-producing cells in both mice and pigs. Here, we provide evidence that the B- and T-cell epitopes need to be tethered to a single molecular platform for successful T-cell help, leading to efficient nAb induction in mice. In addition, mice immunized with a non-covalent mixture of B2T-3A dendrimers containing the B-cell epitopes of FMDV types O and C induced similarly high nAb levels against both serotypes, opening the way for a multivalent vaccine platform against a variety of serologically different FMDVs. These findings are relevant for the design of vaccine strategies based on B- and T-cell epitope combinations.

scholarly journals Detection and Characterization of Functional T-Cell Epitopes on the Structural Proteins VP2, VP3, and VP4 of Foot and Mouth Disease Virus O1 Campos

Virology ◽  
2000 ◽  
Vol 271 (2) ◽  
pp. 234-239 ◽  
Author(s):  
M. Pérez Filgueira ◽  
A. Wigdorovitz ◽  
A. Romera ◽  
P. Zamorano ◽  
M.V. Borca ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
T Cell Epitopes ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease Virus

2001 ◽  
Vol 75 (7) ◽  
pp. 3164-3174 ◽  
Author(s):  
E. Blanco ◽  
M. Garcia-Briones ◽  
A. Sanz-Parra ◽  
P. Gomes ◽  
E. De Oliveira ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals A 10-Amino-Acid Linear Sequence of VP1 of Foot and Mouth Disease Virus Containing B- and T-Cell Epitopes Induces Protection in Mice

Virology ◽  
1995 ◽  
Vol 212 (2) ◽  
pp. 614-621 ◽  
Author(s):  
P. Zamorano ◽  
A. Wigdorovitz ◽  
M. Perez-Filgueira ◽  
C. Carrillo ◽  
J.M. Escribano ◽  
...  
Keyword(s):  
Amino Acid ◽  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Linear Sequence ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Immunogenicity of adenovirus-derived porcine parvovirus-like particles displaying B and T cell epitopes of foot-and-mouth disease

Vaccine ◽  
2016 ◽  
Vol 34 (4) ◽  
pp. 578-585 ◽  
Author(s):  
Qunxing Pan ◽  
Hui Wang ◽  
Wei Ouyang ◽  
Xiaoli Wang ◽  
Zhenwei Bi ◽  
...  
Keyword(s):  
T Cell ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Porcine Parvovirus ◽  
T Cell Epitopes ◽  
Foot And Mouth

Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine

2006 ◽  
Vol 121 (2) ◽  
pp. 223-228 ◽  
Author(s):  
Wilhelm Gerner ◽  
Michael S. Denyer ◽  
Haru-Hisa Takamatsu ◽  
Thomas E. Wileman ◽  
Karl-Heinz Wiesmüller ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Miniature Swine ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Induction of an antigen-specific immune response and partial protection of cattle against challenge infection with foot-and-mouth disease virus (FMDV) after lipopeptide vaccination with FMDV-specific B-cell epitopes

2003 ◽  
Vol 84 (12) ◽  
pp. 3315-3324 ◽  
Author(s):  
Bettina-Judith Höhlich ◽  
Karl-Heinz Wiesmüller ◽  
Bernd Haas ◽  
Wilhelm Gerner ◽  
Roberto Correa ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
B Cell ◽  
Antibody Titre ◽  
Foot And Mouth Disease ◽  
Challenge Infection ◽  
Mouth Disease ◽  
B Cell Epitopes ◽  
Homologous Virus ◽  
Foot And Mouth

To evaluate the potential of chemically synthesized lipopeptides for vaccination against foot-and-mouth disease (FMD), seven lipopeptides containing the immunostimulating principle of bacterial lipoproteins and linear B-cell epitopes of FMDV strain O1Kaufbeuren (O1K) were used to immunize cattle (n=7). Animals were vaccinated once and 21 days after immunization animals were infected with the homologous virus. Four animals were protected. After vaccination, as well as after challenge infection, B- and T-cell responses were examined. Sera were tested for virus- and peptide-specific antibodies and showed after vaccination only a weak antibody response. After challenge infection, an increase in antibody titre was obvious but there was no correlation between antibody titre and protection. The reactivity of the cellular immune system was detected by analyses of PBMCs for virus- and peptide-specific T-lymphocytes. With regard to the virus-specific T-lymphocytes, a heterogeneous reactivity could be shown. No correlation between virus-specific T-cell proliferation and protection was found. Obvious was the fact that all protected animals showed after vaccination a strong T-cell response against at least one of the peptides used for immunization. These results suggest a correlation between the onset of an antigen-specific T-cell reaction and protection.

scholarly journals Peptide-Based Vaccines: Foot-and-Mouth Disease Virus, a Paradigm in Animal Health

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 477
Author(s):  
Mar Forner ◽  
Rodrigo Cañas-Arranz ◽  
Sira Defaus ◽  
Patricia de León ◽  
Miguel Rodríguez-Pulido ◽  
...  
Keyword(s):  
T Cell ◽  
Animal Health ◽  
Foot And Mouth Disease ◽  
Infectious Agent ◽  
Mouth Disease ◽  
Cold Chain ◽  
Attractive Alternative ◽  
T Cell Epitopes ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Vaccines are considered one of the greatest global health achievements, improving the welfare of society by saving lives and substantially reducing the burden of infectious diseases. However, few vaccines are fully effective, for reasons ranging from intrinsic limitations to more contingent shortcomings related, e.g., to cold chain transport, handling and storage. In this context, subunit vaccines where the essential antigenic traits (but not the entire pathogen) are presented in rationally designed fashion have emerged as an attractive alternative to conventional ones. In particular, this includes the option of fully synthetic peptide vaccines able to mimic well-defined B- and T-cell epitopes from the infectious agent and to induce protection against it. Although, in general, linear peptides have been associated to low immunogenicity and partial protection, there are several strategies to address such issues. In this review, we report the progress towards the development of peptide-based vaccines against foot-and-mouth disease (FMD) a highly transmissible, economically devastating animal disease. Starting from preliminary experiments using single linear B-cell epitopes, recent research has led to more complex and successful second-generation vaccines featuring peptide dendrimers containing multiple copies of B- and T-cell epitopes against FMD virus or classical swine fever virus (CSFV). The usefulness of this strategy to prevent other animal and human diseases is discussed.

Sign in / Sign up

Export Citation Format

Share Document