scholarly journals Induction of an antigen-specific immune response and partial protection of cattle against challenge infection with foot-and-mouth disease virus (FMDV) after lipopeptide vaccination with FMDV-specific B-cell epitopes

2003 ◽  
Vol 84 (12) ◽  
pp. 3315-3324 ◽  
Author(s):  
Bettina-Judith Höhlich ◽  
Karl-Heinz Wiesmüller ◽  
Bernd Haas ◽  
Wilhelm Gerner ◽  
Roberto Correa ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
B Cell ◽  
Antibody Titre ◽  
Foot And Mouth Disease ◽  
Challenge Infection ◽  
Mouth Disease ◽  
B Cell Epitopes ◽  
Homologous Virus ◽  
Foot And Mouth

To evaluate the potential of chemically synthesized lipopeptides for vaccination against foot-and-mouth disease (FMD), seven lipopeptides containing the immunostimulating principle of bacterial lipoproteins and linear B-cell epitopes of FMDV strain O1Kaufbeuren (O1K) were used to immunize cattle (n=7). Animals were vaccinated once and 21 days after immunization animals were infected with the homologous virus. Four animals were protected. After vaccination, as well as after challenge infection, B- and T-cell responses were examined. Sera were tested for virus- and peptide-specific antibodies and showed after vaccination only a weak antibody response. After challenge infection, an increase in antibody titre was obvious but there was no correlation between antibody titre and protection. The reactivity of the cellular immune system was detected by analyses of PBMCs for virus- and peptide-specific T-lymphocytes. With regard to the virus-specific T-lymphocytes, a heterogeneous reactivity could be shown. No correlation between virus-specific T-cell proliferation and protection was found. Obvious was the fact that all protected animals showed after vaccination a strong T-cell response against at least one of the peptides used for immunization. These results suggest a correlation between the onset of an antigen-specific T-cell reaction and protection.

scholarly journals Identification of Foot-and-Mouth Disease Virus-Specific Linear B-Cell Epitopes To Differentiate between Infected and Vaccinated Cattle

2003 ◽  
Vol 77 (16) ◽  
pp. 8633-8639 ◽  
Author(s):  
Bettina-Judith Höhlich ◽  
Karl-Heinz Wiesmüller ◽  
Tobias Schlapp ◽  
Bernd Haas ◽  
Eberhard Pfaff ◽  
...  
Keyword(s):  
B Cell ◽  
Foot And Mouth Disease ◽  
The United States ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infected Cattle ◽  
B Cell Epitopes ◽  
Foot And Mouth ◽  
Fmdv Strain ◽  
Linear B

ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. For several years, vaccination of animals, which had proven to be successful for the eradication of the disease, has been forbidden in the United States and the European Community because of the difficulty of differentiating between vaccinated and infected animals. In this study, detailed investigations of the bovine humoral immune response against FMD virus (FMDV) were performed with the aim of identifying viral epitopes recognized specifically by sera derived from FMDV-infected animals. The use of overlapping 15-mer synthetic peptides, covering the whole open reading frame of FMDV strain O1K in a peptide enzyme-linked immunosorbent assay, allowed the identification of 12 FMDV strain O1K-specific linear B-cell epitopes. Six of these linear B-cell epitopes, located in the nonstructural proteins, were used in further assays to compare the reactivities of sera from vaccinated and infected cattle. Antibodies recognizing these peptides could be detected only in sera derived from infected cattle. In further experiments, the reactivity of the six peptides with sera from animals infected with different strains of FMDV was tested, and strain-independent infection-specific epitopes were identified. Thus, these results clearly demonstrate the ability of a simple peptide-based assay to discriminate between infected and conventionally FMD-vaccinated animals.

scholarly journals B Epitope Multiplicity and B/T Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Esther Blanco ◽  
Carolina Cubillos ◽  
Noelia Moreno ◽  
Juan Bárcena ◽  
Beatriz G. de la Torre ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Neutralizing Antibodies ◽  
Cell Epitope ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitope ◽  
T Cell Epitopes ◽  
B Cell Epitope ◽  
Foot And Mouth

Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines. We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) elicit potent B- and T-cell specific responses and confer protection to viral challenge, while juxtaposition of these epitopes in a linear peptide induces less efficient responses. To assess the relevance of B-cell epitope multivalency, dendrimers bearing two (B2T) or four (B4T) copies of the B-cell epitope from type O FMDV (a widespread circulating serotype) were tested in CD1 mice and showed that multivalency is advantageous over simple B-T-epitope juxtaposition, resulting in efficient induction of neutralizing antibodies and optimal release of IFNγ. Interestingly, the bivalent B2T construction elicited similar or even better B- and T-cell specific responses than tetravalent B4T. In addition, the presence of the T-cell epitope and its orientation were shown to be critical for the immunogenicity of the linear juxtaposed monovalent peptides analyzed in parallel. Taken together, our results provide useful insights for a more accurate design of FMD subunit vaccines.

A peptide construct containing B-cell and T-cell epitopes from the foot-and-mouth disease viral VP1 protein induces efficient antiviral protection

Vaccine ◽  
1999 ◽  
Vol 17 (6) ◽  
pp. 577-584 ◽  
Author(s):  
O.M Volpina ◽  
A.Yu Surovoy ◽  
M.N Zhmak ◽  
M.A Kuprianova ◽  
D.O Koroev ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Vp1 Protein ◽  
Foot And Mouth

scholarly journals Immunogenicity of Foot-and-Mouth Disease Virus Dendrimer Peptides: Need for a T-Cell Epitope and Ability to Elicit Heterotypic Responses

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4714
Author(s):  
Rodrigo Cañas-Arranz ◽  
Patricia de León ◽  
Sira Defaus ◽  
Elisa Torres ◽  
Mar Forner ◽  
...  
Keyword(s):  
T Cell ◽  
B Cell ◽  
Cell Epitope ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitope ◽  
T Cell Epitopes ◽  
Vaccine Platform ◽  
Mouth Disease Virus ◽  
Foot And Mouth

An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as a foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies of the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140–158)) covalently linked to a heterotypic T-cell epitope from non-structural protein 3A (21–35), henceforth B2T-3A, has previously been shown to elicit high neutralizing antibody (nAb) titers and IFN-γ-producing cells in both mice and pigs. Here, we provide evidence that the B- and T-cell epitopes need to be tethered to a single molecular platform for successful T-cell help, leading to efficient nAb induction in mice. In addition, mice immunized with a non-covalent mixture of B2T-3A dendrimers containing the B-cell epitopes of FMDV types O and C induced similarly high nAb levels against both serotypes, opening the way for a multivalent vaccine platform against a variety of serologically different FMDVs. These findings are relevant for the design of vaccine strategies based on B- and T-cell epitope combinations.

Genome-wide linear B-cell epitopes of enterovirus 71 in a hand, foot and mouth disease (HFMD) population

2018 ◽  
Vol 105 ◽  
pp. 41-48 ◽  
Author(s):  
Huiying Zhang ◽  
Zhigang Song ◽  
Huiju Yu ◽  
Xiaoling Zhang ◽  
Shanshan Xu ◽  
...  
Keyword(s):  
B Cell ◽  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
B Cell Epitopes ◽  
Genome Wide ◽  
Foot And Mouth ◽  
Linear B

scholarly journals B cell epitopes within VP1 of type O foot-and-mouth disease virus for detection of viral antibodies

2010 ◽  
Vol 25 (1) ◽  
pp. 18-26 ◽  
Author(s):  
Shan-dian Gao ◽  
Jun-zheng Du ◽  
Hui-yun Chang ◽  
Guo-zheng Cong ◽  
Jun-jun Shao ◽  
...  
Keyword(s):  
B Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
B Cell Epitopes ◽  
Viral Antibodies ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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