Oral contraceptives and oestrogen replacement therapy as determinants of plasma fibrinogen level and fibrinolytic activity the northern sweden MONICA study

SummaryA method for determining plasma fibrinogen and fibrinogenolytic activity, with epsilonaminocaproic acid (ε-ACA) as an inhibitor of fibrinolysis, in patients with high fibrinolytic activity, is described in detail.The fibrinogen was determined by a modification of Morrison’s syneresis method in parallel in 1. citrated plasma that was incubated for 2 hours at 37° C, after which further digestion was prevented by addition of ε-ACA and is referred to as fibrinogen A, and 2. in citrated plasma prepared from blood collected in tubes containing ε-ACA to prevent activation of plasminogen and is referred to as fibrinogen B. The difference between fibrinogen B and fibrinogen A gives the amount of lysed fibrinogen in 2 hours at 37° C. Fibrinogen B gives the fibrinogen concentration at the moment of sampling.The method is recommended to be used for evaluation of the true plasma fibrinogen level and the degree of fibrinogenolytic activity. This is particularly important during thrombolytic therapy.

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Assessment of Coagulation Disorder in Women Taking Oral Contraceptives

Journal of Bangladesh Society of Physiologist ◽

10.3329/jbsp.v9i1.22786 ◽

2015 ◽

Vol 9 (1) ◽

pp. 1-5

Author(s):

Samsunnahar ◽

Qazi Shamima Akhter ◽

Atiquzzaman ◽

Najneen Akhter ◽

US Naima Begum ◽

...

Keyword(s):

Oral Contraceptives ◽

Fibrinogen Level ◽

Oral Contraceptive Pill ◽

Plasma Fibrinogen ◽

Total Count ◽

Coagulation Disorder ◽

User Group ◽

Plasma Fibrinogen Level ◽

Cross Sectional ◽

Apparently Healthy

Background: Oral contraceptive pill (OCP) is related to development of hypercoagulability and the risk of thromboembolic effects in women. Objectives: To assess the coagulability status in women taking oral contraceptives by estimation of plasma fibrinogen level and total count of platelets. Methods: This cross sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka, during the period of January 2012 to December 2012 on sixty apparently healthy female subjects aged 25 to 45 years taking OCPs for 1 to 10 years. Thirty subjects were 1 to 5 years user group and another 30 subjects were >5 to 10 years user group. They were collected from family planning clinics of Dhaka city. In addition, age matched 30 apparently healthy women of OCP nonusers were also studied as control. For assessment of coagulability state, plasma fibrinogen and total counts of platelet levels were estimated by automated coagulation analyzer and automated hematology analyzer. Statistical analysis was done by unpaired Students t test. Results: The mean (±SE) total count of platelet level was significantly higher (P<0.001) in contraceptive user group and plasma fibrinogen level was higher in users but it was not significant. Conclusion: This study concludes that OCP users had more tendency of hypercoagulability and therefore these women are at higher risk of thromboembolic effects. DOI: http://dx.doi.org/10.3329/jbsp.v9i1.22786 Bangladesh Soc Physiol. 2014, June; 9(1): 1-5

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Platelet Adhesiveness and Fibrinolysis after Recent Cerebro-Vascular Accidents and their Relationship with Subsequent Deep Venous Thrombosis of the Legs

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648016 ◽

1976 ◽

Vol 36 (01) ◽

pp. 127-132 ◽

Cited By ~ 11

Author(s):

C. P Warlow ◽

J. A. N Rennie ◽

D Ogston ◽

A. S Douglas

Keyword(s):

Platelet Count ◽

Venous Thrombosis ◽

Deep Venous Thrombosis ◽

Plasminogen Activator ◽

Fibrinogen Level ◽

Plasma Fibrinogen ◽

Platelet Adhesiveness ◽

Plasma Fibrinogen Level ◽

Vascular Accidents ◽

Subsequent Rise

SummaryIn fifteen patients with a cerebro-vascular accident resulting in an acute hemiplegia there was a subsequent rise in the platelet count and plasma fibrinogen level. There were no significant alterations in platelet adhesiveness, plasminogen activator, plasminogen, FR-antigen and haematocrit. Patients diagnosed as developing deep venous thrombosis with the 125I-fibrinogen technique had a significantly lower platelet adhesiveness and plasminogen level than those who were not.

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Variation in the Promoter Region of the β Fibrinogen Gene Is Associated with Plasma Fibrinogen Levels in Smokers and Non-Smokers

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648177 ◽

1991 ◽

Vol 65 (05) ◽

pp. 487-490 ◽

Cited By ~ 115

Author(s):

A E Thomas ◽

F R Green ◽

C H Kelleher ◽

H C Wilkes ◽

P J Brennan ◽

...

Keyword(s):

Heart Disease ◽

Promoter Region ◽

Fragment Length Polymorphism ◽

Fibrinogen Level ◽

Plasma Fibrinogen ◽

Plasma Fibrinogen Level ◽

Healthy Men ◽

History Of ◽

General Practices ◽

Restriction Fragment Length

SummaryWe investigated the association between fibrinogen levels and a HaeIII restriction fragment length polymorphism located at −453 bp from the start of transcription of the β fibrinogen gene. 292 healthy men aged 45 to 69 years, recruited from general practices throughout Britain, were studied. None had a history of ischaemic heart disease. 41.1% (120) were smokers and fibrinogen levels were higher in this group. The frequency of the noncutting allele (designated H2) was 0.19 and was the same in smokers and non-smokers. The H2 allele was associated with elevated levels of fibrinogen in both smokers and non-smokers and the effect of genotype was similar in both groups. After smoking, HaeIII genotype was the strongest predictor of fibrinogen levels and explained 3.1% of the variance in fibrinogen levels. These results confirm earlier studies that variation at the fibrinogen locus contributes to the between-individual differences in plasma fibrinogen level.

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A Six Year Prospective Study of Fibrinogen and Other Risk Factors Associated with Mortality in Stable Claudicants

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656361 ◽

1992 ◽

Vol 68 (03) ◽

pp. 261-263 ◽

Cited By ~ 71

Author(s):

A K Banerjee ◽

J Pearson ◽

E L Gilliland ◽

D Goss ◽

J D Lewis ◽

...

Keyword(s):

Risk Factors ◽

Intermittent Claudication ◽

Fibrinogen Level ◽

Past History ◽

Fold Increase ◽

Arterial Disease ◽

Plasma Fibrinogen ◽

Plasma Fibrinogen Level ◽

Factors Associated ◽

Probability Of Death

SummaryA total of 333 patients with stable intermittent claudication at recruitment were followed up for 6 years to determine risk factors associated with subsequent mortality. Cardiovascular diseases were the underlying cause of death in 78% of the 114 patients who died. The strongest independent predictor of death during the follow-up period was the plasma fibrinogen level, an increase of 1 g/l being associated with a nearly two-fold increase in the probability of death within the next 6 years. Age, low ankle/brachial pressure index and a past history of myocardial infarction also increased the probability of death during the study period. The plasma fibrinogen level is a valuable index of those patients with stable intermittent claudication at high risk of early mortality. The results also provide further evidence for the involvement of fibrinogen in the pathogenesis of arterial disease.

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