Influence of organic and ammonia loading on nitrifier activity and nitrification performance for a two-stage biological aerated filter system

In this study, the alternating 3-stage biological aerated filter system with the brush as the filler was proposed for campus sewage treatment and the biofilm formation process was researched. Adopting the four-stage inoculated biofilm formation method, the treatment effects of the 3 filter columns respectively lasted 23d, 20d, 23d to reach stable. After the attached biofilm grew steadily, the effluent COD concentration was between 45 mg/L and 95 mg/L, and the removal efficiency was 77%~85%. The effluent NH4+-N concentration of 1st and 3rd filter columns was 11~25 mg/L, and the removal efficiency was 47%~67%; while the effluent NH4+-N concentration of 2nd filter column was 8 ~19 mg/L, the removal efficiency was 64%~ 78%.

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Correlating Microbial Community Characteristics with Environmental Factors along a Two-Stage Biological Aerated Filter

Water ◽

10.3390/w12123317 ◽

2020 ◽

Vol 12 (12) ◽

pp. 3317

Author(s):

Yuchen An ◽

Songmin Li ◽

Xiaoling Wang ◽

Yuyang Liu ◽

Ruonan Wang

Keyword(s):

Microbial Community ◽

Environmental Factors ◽

High Throughput Sequencing ◽

Pollutant Removal ◽

Organic Load ◽

Biological Aerated Filter ◽

Community Characteristics ◽

Two Stage ◽

Degrading Bacteria ◽

Aerated Filter

The purification effect of a biological aerated filter (BAF) mainly comes from the microorganisms in the reactor. Understanding the correlation between microbial community characteristics and environmental factors along the filter has great significance for maintaining good operation and improving the removal efficiency of the filter. A two-stage BAF was employed to treat domestic sewage under organic loads of 1.02 and 1.55 kg/m3·d for 15 days each. 16S rDNA high-throughput sequencing technology and redundancy analysis were applied to explore the correlation between microbial community characteristics and environmental variables. The results showed that: (1) the crucial organic-degrading bacteria in the A-stage filter were of the genus Novosphingobium, which had a significant increase in terms of relative abundance at sampling outlet A3 (135 cm of the filling height) after the increase of organic load; (2) the microbial communities at different positions in the B-stage filter were similarly affected by environmental factors, and the main bacteria associated with nitrogen removal in the B-stage filter were Zoogloea and Rhodocyclus; and (3) to improve the pollutant removal performance of this two-stage biological aerated filter, a strategy of adding an internal circulation in the B-stage filter can be adopted.

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Influence of organic and ammonia loading on nitrifier activity and nitrification performance for a two-stage biological aerated filter system

Water Science & Technology ◽

10.2166/wst.1999.0363 ◽

1999 ◽

Vol 39 (7) ◽

pp. 227-234 ◽

Cited By ~ 10

Author(s):

K. R. Gilmore ◽

K. J. Husovitz ◽

T. Holst ◽

N. G. Love

Keyword(s):

Water Quality ◽

Ammonia Oxidation ◽

Oligonucleotide Probe ◽

Treatment Plant ◽

Ammonia Oxidizing Bacteria ◽

Biological Aerated Filter ◽

Two Stage ◽

Second Stage ◽

Oxidation Stage ◽

Aerated Filter

A pilot-scale, two-stage (carbon oxidation stage one, ammonia oxidation stage two) fixed film biological aerated filter (BAF) process was operated during the wintertime on-site at a domestic wastewater treatment plant. Over the study period, hydraulic loadings to the system were varied and generated a range of organic and ammonia loading conditions. Nitrification performance was monitored based on water quality along the length of the filters, effluent water quality, and activity levels of ammonia-oxidizing bacteria within the biofilm using an oligonucleotide probe. Overall nitrification efficiency for wintertime conditions (average temperature 12.4 ± 0.1°C) was greater than 90 percent when ammonia-N loadings to the second stage were 0.6 kg/m3-day or less. Nitrification efficiency started to deteriorate at loadings beyond this point. Biofilm and liquid samples were collected along the distance of the two columns at high and low ammonia loadings. The degree of activity observed by ammonia oxidizing bacteria in the biofilm corresponded with the disappearance of ammonia and the generation of nitrate as water passed through the columns. The zones of ammonia oxidizing activity progressed along the length of the columns as organic and ammonia loadings to the system increased. The oligonucleotide probe data suggest that this shift in the location of the nitrifier population is due to higher BOD loads to the second stage, which supported higher levels of heterotrophic growth in the column.

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