scholarly journals Correlating Microbial Community Characteristics with Environmental Factors along a Two-Stage Biological Aerated Filter

Water ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3317
Author(s):  
Yuchen An ◽  
Songmin Li ◽  
Xiaoling Wang ◽  
Yuyang Liu ◽  
Ruonan Wang
Keyword(s):  
Microbial Community ◽  
Environmental Factors ◽  
High Throughput Sequencing ◽  
Pollutant Removal ◽  
Organic Load ◽  
Biological Aerated Filter ◽  
Community Characteristics ◽  
Two Stage ◽  
Degrading Bacteria ◽  
Aerated Filter

The purification effect of a biological aerated filter (BAF) mainly comes from the microorganisms in the reactor. Understanding the correlation between microbial community characteristics and environmental factors along the filter has great significance for maintaining good operation and improving the removal efficiency of the filter. A two-stage BAF was employed to treat domestic sewage under organic loads of 1.02 and 1.55 kg/m3·d for 15 days each. 16S rDNA high-throughput sequencing technology and redundancy analysis were applied to explore the correlation between microbial community characteristics and environmental variables. The results showed that: (1) the crucial organic-degrading bacteria in the A-stage filter were of the genus Novosphingobium, which had a significant increase in terms of relative abundance at sampling outlet A3 (135 cm of the filling height) after the increase of organic load; (2) the microbial communities at different positions in the B-stage filter were similarly affected by environmental factors, and the main bacteria associated with nitrogen removal in the B-stage filter were Zoogloea and Rhodocyclus; and (3) to improve the pollutant removal performance of this two-stage biological aerated filter, a strategy of adding an internal circulation in the B-stage filter can be adopted.

Effect of aeration rates on hydraulic characteristics and pollutant removal in an up-flow biological aerated filter

2018 ◽  
Vol 4 (12) ◽  
pp. 2041-2050 ◽  
Author(s):  
Jiehui Ren ◽  
Wen Cheng ◽  
Tian Wan ◽  
Min Wang ◽  
Meng Jiao
Keyword(s):  
Microbial Community ◽  
Flow Field ◽  
Dissolved Oxygen ◽  
Pollutant Removal ◽  
Biological Aerated Filter ◽  
Hydraulic Characteristics ◽  
Aerated Filter ◽  
Distribution Of Flow

Aeration rates could improve the pollutant removal by forming the suitable distribution of flow field, dissolved oxygen and microbial community.

Greywater treatment with biological aerated filter (BAF) for urban water reuse

2010 ◽  
Vol 10 (6) ◽  
pp. 907-914
Author(s):  
A. Meda ◽  
P. Cornel
Keyword(s):  
Fresh Water ◽  
Water Reuse ◽  
Treatment Plant ◽  
Organic Load ◽  
Design Parameters ◽  
Urban Water ◽  
Biological Aerated Filter ◽  
Service Water ◽  
Aerated Filter ◽  
Set Up

Greywater is an excellent resource for service water in intra-urban reuse. By substituting fresh water with appropriately treated greywater, it is possible to save 30–50% of fresh water. In this paper, an up-flow pilot biological aerated filter (BAF) is tested for the treatment of synthetic greywater and the design parameters are determined. An organic load of 8 kg COD/(m3 d) allows to achieving a good effluent quality with regard to COD, suspended solids, and anionic surfactants. By reducing the organic load to 5 kg COD/(m3 d) full nitrification is achieved as well. A process set-up for a full-scale BAF greywater treatment plant is proposed and dimensioned using the parameters from the pilot plant tests. Here, the required specific volume is approx. 3 L/C for the reactor and 16 L/C for the storage tanks. This feature make the BAF a compact process which can be successfully integrated in intra-urban water reuse schemes.

Influence of organic and ammonia loading on nitrifier activity and nitrification performance for a two-stage biological aerated filter system

1999 ◽  
Vol 39 (7) ◽  
pp. 227-234 ◽  
Author(s):  
K. R. Gilmore ◽  
K. J. Husovitz ◽  
T. Holst ◽  
N. G. Love
Keyword(s):  
Water Quality ◽  
Ammonia Oxidation ◽  
Oligonucleotide Probe ◽  
Treatment Plant ◽  
Ammonia Oxidizing Bacteria ◽  
Biological Aerated Filter ◽  
Two Stage ◽  
Second Stage ◽  
Oxidation Stage ◽  
Aerated Filter

A pilot-scale, two-stage (carbon oxidation stage one, ammonia oxidation stage two) fixed film biological aerated filter (BAF) process was operated during the wintertime on-site at a domestic wastewater treatment plant. Over the study period, hydraulic loadings to the system were varied and generated a range of organic and ammonia loading conditions. Nitrification performance was monitored based on water quality along the length of the filters, effluent water quality, and activity levels of ammonia-oxidizing bacteria within the biofilm using an oligonucleotide probe. Overall nitrification efficiency for wintertime conditions (average temperature 12.4 ± 0.1°C) was greater than 90 percent when ammonia-N loadings to the second stage were 0.6 kg/m3-day or less. Nitrification efficiency started to deteriorate at loadings beyond this point. Biofilm and liquid samples were collected along the distance of the two columns at high and low ammonia loadings. The degree of activity observed by ammonia oxidizing bacteria in the biofilm corresponded with the disappearance of ammonia and the generation of nitrate as water passed through the columns. The zones of ammonia oxidizing activity progressed along the length of the columns as organic and ammonia loadings to the system increased. The oligonucleotide probe data suggest that this shift in the location of the nitrifier population is due to higher BOD loads to the second stage, which supported higher levels of heterotrophic growth in the column.

scholarly journals Multistage A-O Activated Sludge Process for Paraformaldehyde Wastewater Treatment and Microbial Community Structure Analysis

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Danyang Zheng ◽  
Yujiao Sun ◽  
Huijuan Li ◽  
Sidan Lu ◽  
Mingjun Shan ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Activated Sludge ◽  
Microbial Diversity ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Diversity Index ◽  
Public Health Issue ◽  
Activated Sludge Process ◽  
Degrading Bacteria

In recent years, the effect of formaldehyde on microorganisms and body had become a global public health issue. The multistage combination of anaerobic and aerobic process was adopted to treat paraformaldehyde wastewater. Microbial community structure in different reaction stages was analyzed through high-throughput sequencing. Results showed that multistage A-O activated sludge process positively influenced polyformaldehyde wastewater. The removal rates of formaldehyde were basically stable at more than 99% and those of COD were about 89%. Analysis of the microbial diversity index indicated that the microbial diversity of the reactor was high, and the treatment effect was good. Moreover, microbial community had certain similarity in the same system. Microbial communities in different units also showed typical representative characteristics affected by working conditions and influent concentrations. Proteobacteria, Firmicutes, and Bacteroidetes were the dominant fungal genera in the phylum level of community composition. As to family and genus levels, Peptostreptococcaceae was distributed at various stages and the dominant in this system. This bacterium also played an important role in organic matter removal, particularly decomposition of the acidified middle metabolites. In addition, Rhodobacteraceae and Rhodocyclaceae were the formaldehyde-degrading bacteria found in the reactor.

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