The growth inhibitory effect of conjugated linoleic acid on MCF-7 cells is related to estrogen response system

1997 ◽  
Vol 116 (2) ◽  
pp. 121-130 ◽  
Author(s):  
Vijayender Rao Durgam ◽  
Gabriel Fernandes
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Inhibitory Effect ◽  
Growth Inhibitory Effect ◽  
Response System ◽  
Estrogen Response ◽  
Growth Inhibitory ◽  
Mcf 7

Reduction of linoleic acid inhibition in production of conjugated linoleic acid byPropionibacterium freudenreichiissp.shermanii

2001 ◽  
Vol 47 (8) ◽  
pp. 735-740 ◽  
Author(s):  
A Rainio ◽  
M Vahvaselkä ◽  
T Suomalainen ◽  
S Laakso
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Inhibitory Effect ◽  
Propionibacterium Freudenreichii ◽  
Growth Inhibitory Effect ◽  
Sorbitan Monooleate ◽  
Whey Permeate ◽  
Growth Inhibitory ◽  
De Man ◽  
Sufficient Concentration

A method for the production of conjugated linoleic acid (CLA) from linoleic acid (LA) using growing cultures of Propionibacterium freudenreichii ssp. shermanii JS was developed. The growth inhibitory effect of LA was eliminated by dispersing it in a sufficient concentration of polyoxyethylene sorbitan monooleate detergent. For the whey permeate medium used, the optimum LA:detergent ratio was 1:15 (w/w). As a result, the cultures tolerated at least 1000 µg·mL–1LA, which was converted to CLA with 57%–87% efficiency. The cis-9, trans-11 and trans-9, cis-11 isomers constituted 85%–90% of the CLA produced. The feasibility of the method was demonstrated also in de Man – Rogosa–Sharpe (MRS) broth.Key words: conjugated linoleic acid, linoleic acid, Propionibacterium freudenreichii ssp. shermanii.

scholarly journals Involvement of PPARα in the growth inhibitory effect of arachidonic acid on breast cancer cells

2008 ◽  
Vol 100 (4) ◽  
pp. 739-750 ◽  
Author(s):  
Claudia Bocca ◽  
Francesca Bozzo ◽  
Germana Martinasso ◽  
Rosa Angela Canuto ◽  
Antonella Miglietta
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Inhibitory Effect ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Growth Inhibitory Effect ◽  
Growth Inhibitory ◽  
Mcf 7

Epidemiological studies suggest that dietary PUFA may influence breast cancer progression. n-3 PUFA are generally known to exert antitumour effects, whereas reports relative to n-6 PUFA anti-carcinogen effects are controversial. Arachidonic acid (AA; 20 : 4n − 6) and its metabolites have been shown to inhibit the growth of human breast cancer cell lines, even if the downstream mechanisms by which AA may influence carcinogenesis remain unresolved. We explored the molecular basis for AA influence on proliferation, signal transduction and apoptosis in two human breast cancer cell lines, MCF-7 and MDA-MB-231. In both cell lines AA inhibited cell growth in a dose-dependent manner, even if MDA-MB-231 was somewhat more growth-inhibited than MCF-7. AA decreased extracellular signal-regulated protein kinase 1/2 phosphorylation level, and positively modulated PPARγ and PPARα expression, with only a slight effect against PPARβ/δ. In addition, AA increased Bak (an apoptosis-regulating protein) expression and reduced procaspase-3 and -9 levels only in MDA-MB-231 cells, thus indicating that the growth inhibitory effect can be correlated with apoptosis induction. In both cell lines the use of a specific antagonist made it possible to establish a relationship between AA growth inhibitory effect and PPARα involvement. AA decreases cell proliferation most likely by inducing apoptosis in MDA-MB-231 cells, while in the MCF-7 cell line the growth inhibitory activity can be attributed to the inhibition of the signal transduction pathway involved in cell proliferation. In both cases, the results here presented suggest PPARα as a possible contributor to the growth inhibitory effect of AA.

scholarly journals Combination of Leunca Herb Ethanolic Extract and Doxorubicin Suppresses HeLa Cells’ Growth

2011 ◽  
Vol 2 (3) ◽  
pp. 281
Author(s):  
Sarmoko Sarmoko ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Endah Puspitasari ◽  
Anindyajati Anindyajati ◽  
Edy Meiyanto
Keyword(s):  
Mtt Assay ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Hela Cell Line ◽  
Growth Inhibitory Effect ◽  
Chemopreventive Agent ◽  
Growth Inhibitory ◽  
Solanum Nigrum L ◽  
Ht 29 ◽  
Mcf 7

Leunca (Solanum nigrum L.)ethanolic extractshowedcytotoxic activity on several cancer cell lines (HepG2, HT-29) and showed anti-proliferative activityon MCF-7 cells. Its application as a combinationagent in chemotherapy will increase the effectivity and reduce the toxicity of chemotherapy. We predict that application of combinatorial chemotherapy in cancer treatment will be more effective and less toxic compared to single treatment. Our research aims to investigate the cytotoxic activitiy of leunca herbs ethanolic extract alone and in combination with doxorubicin on HeLa cell line. MTT assay was conducted to measure the growth inhibitory effect of leunca herbs ethanolic extract and combinatorial treatments. Leunca herb ethanolic extract (5, 50, 250 μg/ml) increased the cytotoxic effect of  doxorubicin compared to doxorubicin alone. The strongest cytotoxic activity resulted from the combination of 250 μg/ml leunca herbs ethanolic extract and 250 nM doxorubicin. Based on our results, leunca herbs ethanolic extract is a potential chemopreventive agent, while its molecular mechanism needs to be explored.Keywords : Leunca herbs ethanolic extract, doxorubicin, HeLa, MTT assay

scholarly journals Growth-Inhibitory Effect of Chitosan-Coated Liposomes Encapsulating Curcumin on MCF-7 Breast Cancer Cells

Marine Drugs ◽  
2020 ◽  
Vol 18 (4) ◽  
pp. 217 ◽  
Author(s):  
Mahmoud Hasan ◽  
Kamil Elkhoury ◽  
Nabila Belhaj ◽  
Cyril Kahn ◽  
Ali Tamayol ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Anticancer Agents ◽  
Breast Cancer Cells ◽  
Inhibitory Effect ◽  
Control Group ◽  
Growth Inhibitory Effect ◽  
Lower Growth ◽  
Growth Inhibitory ◽  
Mcf 7

Current anticancer drugs exhibit limited efficacy and initiate severe side effects. As such, identifying bioactive anticancer agents that can surpass these limitations is a necessity. One such agent, curcumin, is a polyphenolic compound derived from turmeric, and has been widely investigated for its potential anti-inflammatory and anticancer effects over the last 40 years. However, the poor bioavailability of curcumin, caused by its low absorption, limits its clinical use. In order to solve this issue, in this study, curcumin was encapsulated in chitosan-coated nanoliposomes derived from three natural lecithin sources. Liposomal formulations were all in the nanometric scale (around 120 nm) and negatively charged (around −40 mV). Among the three lecithins, salmon lecithin presented the highest growth-inhibitory effect on MCF-7 cells (two times lower growth than the control group for 12 µM of curcumin and four times lower for 20 µM of curcumin). The soya and rapeseed lecithins showed a similar growth-inhibitory effect on the tumor cells. Moreover, coating nanoliposomes with chitosan enabled a higher loading efficiency of curcumin (88% for coated liposomes compared to 65% for the non-coated liposomes) and a stronger growth-inhibitory effect on MCF-7 breast cancer cells.

scholarly journals AURUM POLYACRYLATE: THE EXPERIMENTAL STUDY OF THE ANTITUMOR ACTIVITY

2020 ◽  
Vol 19 (4) ◽  
pp. 74-85
Author(s):  
L. A. Ostrovskaya ◽  
D. B. Korman ◽  
N. V. Bluhterova ◽  
M. M. Fomina ◽  
V. A. Rikova ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Lung Carcinoma ◽  
Human Tumor ◽  
Inhibitory Effect ◽  
Lewis Lung ◽  
Human Tumor Cells ◽  
Growth Inhibitory Effect ◽  
Growth Inhibitory ◽  
Mcf 7 ◽  
Potential Antitumor

Introduction. The investigation of metal substituted organic compounds as potential antitumor drugs is one of the promising areas of research in experimental and clinical oncology.Objective. The pre-clinical study of the original antitumor drug aurum polyacrylate (aurumacryl) which belongs to such new for oncology group of compounds as polyacrylates of metals was the aim of this work.Materials and methods.  Aurumacryl antitumor activity was determined as the tumor growth inhibitory effect against some of the murine solid tumors (Lewis lung carcinoma, Acatol adenocarcinoma and Ca-755 adenocarcinoma). Drug cytotoxic effect against some of the human tumor cells (Mel-Mo melanoma, A549 lung carcinoma, MCF-7 breast carcinoma, HCT116 colon adenocarcinoma) was evaluated with standard МТТ-test. The aurumacryl pharmacokinetics in tumor bearing mice (Lewis lung carcinoma) was studied. The inductively coupled plasma mass spectrometry method was used for the estimation of the aurum maintenance in the tested tissues (tumor, blood, kidneys, liver, lungs, spleen, brain).Results. The 80–90 % tumor growth inhibitory effect of aurumacryl against some solid tumors in mice had been revealed in vivo as well as the death of the 60–90 % human tumor cells of various origins in vitro. Beside this the strong decrease of the number of proliferating MCF-7 tumor cells had been shown. The distribution of aurumacryl in the body of the mice with the solid tumor had been revealed.Conclusion. On the base of the data obtained the further study of the aurumacryl as a potential antitumor agent seems rather promising.

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