Voluntary wheel running modulates glutamate receptor subunit gene expression and stress hormone release in Lewis rats

Aging leads to sarcopenia and loss of physical function. We examined whether voluntary wheel running, when combined with dietary supplementation with (−)-epigallocatechin-3-gallate (EGCG) and β-alanine (β-ALA), could improve muscle function and alter gene expression in the gastrocnemius of aged mice. Seventeen-month-old BALB/cByJ mice were given access to a running wheel or remained sedentary for 41 days while receiving either AIN-93M (standard feed) or AIN-93M containing 1.5 mg·kg−1 EGCG and 3.43 mg·kg−1 β-ALA. Mice underwent tests over 11 days from day 29 to day 39 of the study period, including muscle function testing (grip strength, treadmill exhaustive fatigue, rotarod). Following a rest day, mice were euthanized and gastrocnemii were collected for analysis of gene expression by quantitative PCR. Voluntary wheel running (VWR) improved rotarod and treadmill exhaustive fatigue performance and maintained grip strength in aged mice, while dietary intervention had no effect. VWR increased gastrocnemius expression of several genes, including those encoding interleukin-6 (Il6, p = 0.001), superoxide dismutase 1 (Sod1, p = 0.046), peroxisome proliferator-activated receptor gamma coactivator 1-α (Ppargc1a, p = 0.013), forkhead box protein O3 (Foxo3, p = 0.005), and brain-derived neurotrophic factor (Bdnf, p = 0.008), while reducing gastrocnemius levels of the lipid peroxidation marker 4-hydroxynonenal (p = 0.019). Dietary intervention alone increased gastrocnemius expression of Ppargc1a (p = 0.033) and genes encoding NAD-dependent protein deacetylase sirtuin-1 (Sirt1, p = 0.039), insulin-like growth factor I (Igf1, p = 0.003), and macrophage marker CD11b (Itgam, p = 0.016). Exercise and a diet containing β-ALA and EGCG differentially regulated gene expression in the gastrocnemius of aged mice, while VWR but not dietary intervention improved muscle function. We found no synergistic effects between dietary intervention and VWR.

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Voluntary Wheel Running Reverses Age-Induced Changes in Hippocampal Gene Expression

PLoS ONE ◽

10.1371/journal.pone.0022654 ◽

2011 ◽

Vol 6 (8) ◽

pp. e22654 ◽

Cited By ~ 44

Author(s):

Rachel A. Kohman ◽

Sandra L. Rodriguez-Zas ◽

Bruce R. Southey ◽

Keith W. Kelley ◽

Robert Dantzer ◽

...

Keyword(s):

Gene Expression ◽

Wheel Running ◽

Voluntary Wheel Running ◽

Induced Changes ◽

Voluntary Wheel

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Voluntary wheel running reduces voluntary consumption of ethanol in mice: identification of candidate genes through striatal gene expression profiling

Genes Brain & Behavior ◽

10.1111/gbb.12294 ◽

2016 ◽

Vol 15 (5) ◽

pp. 474-490 ◽

Cited By ~ 11

Author(s):

T. M. Darlington ◽

R. D. McCarthy ◽

R. J. Cox ◽

J. Miyamoto-Ditmon ◽

X. Gallego ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Candidate Genes ◽

Expression Profiling ◽

Wheel Running ◽

Voluntary Wheel Running ◽

Voluntary Wheel

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Effects of intrinsic aerobic capacity and ovariectomy on voluntary wheel running and nucleus accumbens dopamine receptor gene expression

Physiology & Behavior ◽

10.1016/j.physbeh.2016.06.006 ◽

2016 ◽

Vol 164 ◽

pp. 383-389 ◽

Cited By ~ 17

Author(s):

Young-Min Park ◽

Jill A. Kanaley ◽

Jaume Padilla ◽

Terese Zidon ◽

Rebecca J. Welly ◽

...

Keyword(s):

Gene Expression ◽

Nucleus Accumbens ◽

Dopamine Receptor ◽

Aerobic Capacity ◽

Wheel Running ◽

Receptor Gene ◽

Voluntary Wheel Running ◽

Dopamine Receptor Gene ◽

Receptor Gene Expression ◽

Voluntary Wheel

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Voluntary Wheel Running Did Not Alter Gene Expression in 5xfad Mice, but in Wild-Type Animals Exclusively after One-Day of Physical Activity

Cells ◽

10.3390/cells10030693 ◽

2021 ◽

Vol 10 (3) ◽

pp. 693

Author(s):

Anna Wierczeiko ◽

Lena Gammel ◽

Konstantin Radyushkin ◽

Vu Thu Thuy Nguyen ◽

Hristo Todorov ◽

...

Keyword(s):

Gene Expression ◽

Physical Activity ◽

Preventive Intervention ◽

Wheel Running ◽

Wild Type ◽

Voluntary Wheel Running ◽

5Xfad Mice ◽

Disease Stages ◽

The Impact ◽

Voluntary Wheel

Physical activity is considered a promising preventive intervention to reduce the risk of developing Alzheimer’s disease (AD). However, the positive effect of therapeutic administration of physical activity has not been proven conclusively yet, likely due to confounding factors such as varying activity regimens and life or disease stages. To examine the impact of different routines of physical activity in the early disease stages, we subjected young 5xFAD and wild-type mice to 1-day (acute) and 30-day (chronic) voluntary wheel running and compared them with age-matched sedentary controls. We observed a significant increase in brain lactate levels in acutely trained 5xFAD mice relative to all other experimental groups. Subsequent brain RNA-seq analysis did not reveal major differences in transcriptomic regulation between training durations in 5xFAD mice. In contrast, acute training yielded substantial gene expression changes in wild-type animals relative to their chronically trained and sedentary counterparts. The comparison of 5xFAD and wild-type mice showed the highest transcriptional differences in the chronic and sedentary groups, whereas acute training was associated with much fewer differentially expressed genes. In conclusion, our results suggest that different training durations did not affect the global transcriptome of 3-month-old 5xFAD mice, whereas acute running seemed to induce a similar transcriptional stress state in wild-type animals as already known for 5xFAD mice.

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