Evaluation of the Catalase and Limulus Amoeboctye Lysate Tests for Rapid Determination of The Microbial Quality of Vacuum-Packed Cooked Turkey

AbstractWith the vast improvements in sequencing technologies and increased number of protocols, sequencing is finding more applications to answer complex biological problems. Thus, the amount of publicly available sequencing data has tremendously increased in repositories such as SRA, EGA, and ENCODE. With any large online database, there is a critical need to accurately document study metadata, such as the source protocol and organism. In some cases, this metadata may not be systematically verified by the hosting sites and may result in a negative influence on future studies. Here we present SeqWho, a program designed to heuristically assess the quality of sequencing files and reliably classify the organism and protocol type. This is done in an alignment-free algorithm that leverages a Random Forest classifier to learn from native biases in k-mer frequencies and repeat sequence identities between different sequencing technologies and species. Here, we show that our method can accurately and rapidly distinguish between human and mouse, nine different sequencing technologies, and both together, 98.32%, 97.86%, and 96.38% of the time in high confidence calls respectively. This demonstrates that SeqWho is a powerful method for reliably checking the identity of the sequencing files used in any pipeline and illustrates the program’s ability to leverage k-mer biases.

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Impacto, em curto prazo, dos antibióticos amoxicilina e doxiciclina na qualidade microbiana de um Latossolo Vermelho-amarelo (Short-term impact of the antibiotics amoxicillin and doxycycline on the microbial quality of a Red-yellow Latosol)

Revista Brasileira de Geografia Física ◽

10.26848/rbgf.v12.4.p1340-1354 ◽

2019 ◽

Vol 12 (4) ◽

pp. 1340

Author(s):

Luiz Gustavo Paulon Rezende ◽

Márcia Matiko Kondo ◽

Rogério Melloni

Keyword(s):

Microbial Activity ◽

Soil Microorganisms ◽

Microbial Quality ◽

Environmental Matrices ◽

Short Term ◽

Wide Range ◽

Keywords Antibiotics ◽

The Impact

Concentrações residuais de antibióticos de uso compartilhado pela terapia médica humana e veterinária são cada vez mais frequentes nos mais variados tipos de matrizes ambientais; no entanto, pouco se sabe sobre o impacto que esses fármacos podem acarretar aos microrganismos do solo. Sendo assim, perturbações relacionadas à exposição da microbiota de um latossolo vermelho-amarelo brasileiro a dois antibacterianos, a amoxicilina (AMOX) e a doxiciclina (DOX), foram investigadas por meio da determinação de atividade (mg CO2) e biomassa (Cmic) microbianas, juntamente com o quociente metabólico (qCO2), em amostras de solo que receberam as seguintes concentrações desses compostos: 0,03, 0,3, 3,0, 30 e 300 mg L-1. Os resultados mostraram diferentes efeitos sobre a microbiota e de forma específica para cada antibiótico. A AMOX mostrou-se mais impactante para os microrganismos do solo, com redução da biomassa e aumento do qCO2, enquanto que a DOX reduziu a atividade microbiana, mas sem efeito na biomassa e qCO2.A B S T R A C TThe residual concentrations of antibiotics used by human and veterinary medical therapy are increasingly common in a wide range of environmental matrices, nevertheless little is known about the impact of these drugs on to the soil microorganisms. Therefore, disturbances related to the exposure of the microbiota of a Brazilian Red-yellow Latosol to two antibacterials, amoxicillin (AMOX) and doxycycline (DOX), were investigated through the determination of the microbial activity (mg CO2) and biomass (Cmic), among with the metabolic quotient (qCO2), using soil samples spiked with: 0,03, 0,3, 3,0, 30 and 300 mg L-1 of each drug. The results showed different effects on the microbiota and in a specific way for each antibiotic. The AMOX showed higher impact impacting for the soil microorganisms, with reduction of the biomass and increase of the qCO2, whereas the DOX reduced the microbial activity, but showed no effect in the biomass and qCO2.Keywords: Antibiotics. Amoxicillin. Doxycycline. Bioindicators. Latosols.

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Weak Beam Imaging and Diffraction Studies of Stacking Faults in Silicon

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092918 ◽

1976 ◽

Vol 34 ◽

pp. 590-591

Author(s):

T. Y. Tan ◽

W. K. Tice

Keyword(s):

Fast Neutron ◽

Rapid Determination ◽

Stacking Faults ◽

Imaging Method ◽

Large Reduction ◽

Dislocation Loops ◽

Fringe Spacing ◽

Weak Beam ◽

Kinematical Theory

In studying ion implanted semiconductors and fast neutron irradiated metals, the need for characterizing small dislocation loops having diameters of a few hundred angstrom units usually arises. The weak beam imaging method is a powerful technique for analyzing these loops. Because of the large reduction in stacking fault (SF) fringe spacing at large sg, this method allows for a rapid determination of whether the loop is faulted, and, hence, whether it is a perfect or a Frank partial loop. This method was first used by Bicknell to image small faulted loops in boron implanted silicon. He explained the fringe spacing by kinematical theory, i.e., ≃l/(Sg) in the fault fringe in depth oscillation. The fault image contrast formation mechanism is, however, really more complicated.

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Tingkat Pemahaman Kompetensi Guru Pada Mahasiswa Pendidikan Geografi Sebagai Calon Guru Geografi

JURNAL SERAMBI ILMU ◽

10.32672/si.v21i1.1880 ◽

2020 ◽

Vol 21 (1) ◽

pp. 102-117

Author(s):

Novia Zalmita ◽

Muhajirah Muhajirah ◽

Abdul Wahab Abdi

Keyword(s):

Prospective Teachers ◽

Prospective Teacher ◽

Quantitative Description ◽

Population Data ◽

Geography Education ◽

Teacher Competence ◽

Education Department ◽

Long Time

One that influences human resource indicators is education. The teacher is a profession as a job of academic specialization in a relatively long time in college. Understanding related to teacher competence is very important to have by a prospective teacher because it can affect the quality of performance as a professional teacher. The teacher's competence is known as pedagogic, professional, social and personality competencies. The issue in this study is how the competency of the teacher of the Department of Geography Education FKIP Unsyiah as a prospective teacher of geography? The purpose of this study was to determine the competence of teachers in the Department of Geography Education FKIP Unsyiah as prospective geography teachers. Quantitative description approach is used in this study to find answers to the issue. The population in this study were students of the Department of Geography Education FKIP Unsyiah class of 2015 and 2016 who had been declared to have passed the Micro Teaching and Magang Kependidikan 3 course totaling 50 people. Because the population is small and can be reached, the determination of the sample using total sampling techniques so that the sample in this study is the whole population. Data collection is done by distributing test questions to respondents. The data was analyzed using the descriptive statistics percentage formula. The results of the study indicate that the level of teacher competence of Geography Education Department students as prospective teachers is in the moderate category, namely as many as 22 respondents (44%). A total of 12 respondents (24%) were in the high category, 15 respondents (30%) were in the low category and 1 respondent (2%) were in the very low category.

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