An Electron Microscopic and Biochemical Study of the Effects of Insulin on Newborn Rat Hepatocytes

Previous work has shown that mirex undergoes photolytic dechlorination to chlordecone (CD) (KeponeR) in the environment. Much work has shown that prior exposure to nontoxic levels of CD causes potentiation of hepatotoxicity and lethality of CCl4, BrCCl3 and other halomethane compounds. Potentiation of bromotrichloromethane hepatotoxicity has been associated with compounds that stimulate the activity of hepatic mixed-function oxidase (MFO). An increase in the metabolism of halomethane by the MFO to a free radical initiates peroxidative decomposition of membranal lipids ending in massive cellular injury. However, not all MFO inducers potentiate BrCCl3 hepatotoxicity. Potentiation by much larger doses of phenobarbital is minimal and th at by a more potent inducer of MFO, mirex, is negligible at low doses. We suggest that the CD and bromotrichloromethane interaction results in a depletion of cellular energy and thereby reducing the cellular ability to undergo mitosis.

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Scanning electron microscopic studies on the cytolytic effect of phallolysin on isolated rat hepatocytes and AS-30 D hepatoma cells

Naunyn-Schmiedeberg s Archives of Pharmacology ◽

10.1007/bf00505088 ◽

1976 ◽

Vol 295 (3) ◽

pp. 211-213 ◽

Cited By ~ 26

Author(s):

E. Petzinger ◽

Ruth Seeger

Keyword(s):

Rat Hepatocytes ◽

Hepatoma Cells ◽

Electron Microscopic ◽

Isolated Rat Hepatocytes ◽

Scanning Electron Microscopic ◽

Microscopic Studies ◽

Isolated Rat ◽

Scanning Electron

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Localization of transforming growth factor-beta 1 in mitochondria of murine heart and liver.

Cell Regulation ◽

10.1091/mbc.2.6.467 ◽

1991 ◽

Vol 2 (6) ◽

pp. 467-477 ◽

Cited By ~ 61

Author(s):

U I Heine ◽

J K Burmester ◽

K C Flanders ◽

D Danielpour ◽

E F Munoz ◽

...

Keyword(s):

Growth Factor ◽

Transforming Growth Factor Beta ◽

Transforming Growth Factor ◽

Polyclonal Antibodies ◽

Rat Hepatocytes ◽

Cell Fractionation ◽

Tgf Beta ◽

Electron Microscopic ◽

Mitochondrial Fractions ◽

Growth Factor Beta

Using both electron microscopic immunohistochemistry and cell fractionation techniques, we show that transforming growth factor-beta 1 (TGF-beta 1) is found in mitochondria of rat and mouse cardiac myocytes and rat hepatocytes. Four different polyclonal antibodies, raised against various epitopes encompassing the mature portion of the TGF-beta 1 molecule as well as the pro-region of its precursor, were used for the electron microscopy studies. The localization of TGF-beta 1 in mitochondria was confirmed by detection of the native peptide in mitochondria isolated from rat heart and liver; the majority of native TGF-beta 1 found in liver homogenates was recovered in highly pure mitochondrial fractions. The functional role of TGF-beta in the mitochondrion is unknown at present.

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The Pathobiology of Human Neuromas: An Electron Microscopic and Biochemical Study

Journal of Hand Surgery (European Volume) ◽

10.1016/s0266-7681(85)80015-2 ◽

1985 ◽

Vol 10 (1) ◽

pp. 49-53 ◽

Cited By ~ 17

Author(s):

MARIE A. BADALAMENTE ◽

L. C. HURST ◽

J. ELLSTEIN ◽

C. A. McDEVITT

Keyword(s):

Alcian Blue ◽

Biochemical Study ◽

Blue Staining ◽

Alcian Blue Staining ◽

Post Injury ◽

Electron Microscopic ◽

Biochemical Analyses

The formation of neuroma scar after trauma or neurorrhaphy is believed to be mediated by the response of collagen forming fibroblasts. In this study of twenty human neuromas, myofibroblasts were identified as a component of the scar. These cells occurred singly or as aggregates. There was a qualitative increase of myofibroblasts during the period from two to six months post-injury. From six months to one and one-half years post-injury, numbers and aggregations of myofibroblasts diminished, concurrent with collagen proliferation. Ultrastructural alcian blue staining and biochemical analyses revealed a glucosamine—glycosaminoglycan matrix within neuromas when compared to control nerves. Myofibroblasts appear to play a part in the pathobiology of human neuromas.

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