Antifungal activity of tomato seed extracts

The current study investigated the phytochemical analysis, chemical composition, antioxidant, cytotoxic and antifungal activity of Trichilia emetica seed extracts. Preliminary phytochemical screening and quantification were realized by GC-MS. Consecutive extractions (methanol, ethanol, ethyl acetate, hexane, chloroform and distilled water) were carried out and phytochemical tests performed to represent the variety of phytochemicals. Seed extracts variably displayed alkaloids, cardiac glycosides, phenols, sterols, terpenoids and flavonoids but there was no indication of saponins and tannins in all extracts. The biological investigation of the methanolic extract showed significant biological activities. The results obtained revealed that methanol extract has presented significant IC50 =5.94 µg/mL radical scavenging activity. Minimum inhibitory concentration (MIC) of hexane and chloroform fractions displayed antifungal activity (40.95-100 and 76.27-100.11, respectively) against all three fungal pathogens, whilst the methanol extract inhibited C. krusei and C. parapsilosis and the ethyl acetate inhibited C. parapsilosis only. The results obtained revealed the medicinal importance of the plant and methanol extract of T. emetica would aid researchers to exploit as potential antifungal and antioxidant agent.

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Daily ingestion of nutraceutical-containing proteins, marine polysaccharides, grape and tomato seed extracts, vitamin C, and zinc for the treatment of skin aging and aesthetic quality in men

Journal of the American Academy of Dermatology ◽

10.1016/j.jaad.2011.11.114 ◽

2012 ◽

Vol 66 (4) ◽

pp. AB25

Keyword(s):

Vitamin C ◽

Skin Aging ◽

Tomato Seed ◽

Aesthetic Quality ◽

Daily Ingestion ◽

Seed Extracts

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Phytochemicals Screening and Antifungal Activity of Balanites aegyptiaca Seed and Callus Extract against Candida albicans

Asian Plant Research Journal ◽

10.9734/aprj/2020/v4i430091 ◽

2020 ◽

pp. 9-16

Author(s):

Ayuba Kalum Abaka ◽

Gali Adamu Ishaku ◽

Aishatu Haruna ◽

Bello Pariya Ardo

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Methanolic Extract ◽

Antimicrobial Effect ◽

Seed Kernel ◽

Zone Of Inhibition ◽

Balanites Aegyptiaca ◽

Plant Materials ◽

Zones Of Inhibition ◽

Seed Extracts

Balanites aegyptiaca is a medicinal plant that serves as a source of phytochemicals with antimicrobial effect. This work aimed at screening for phytochemical constituents and investigating the antifungal activity of B. aegyptiaca seed and callus extract against Candida albicans. Callus induction from B. aegyptiaca seed kernel explant was done on MS basal nutrient medium supplemented with 0.5 BAP + 1.0 2, 4-D + 1.0 NAA. Cultures were kept under a controlled temperature and light conditions for five weeks. Plant materials were extracted using solvent extraction. 50 ml of the solvents: methanol and n-hexane were mixed with five grams (5 g) each of the grounded plant materials (1:10) w/v. Determination of antimicrobial activity was done using disc diffusion assay. Diffusion discs of approximately 6 mm diameter were prepared from Whatman No. 1 filter paper, then sterilized and autoclaved before drying in an oven. 10 µl of 50 and 100 mg/ml concentration of each crude extracts was impregnated on separate sterile disc using sterile micropipette tips. The diameter of zone of inhibition at 100 and 50 mg/ml showed the methanolic extract of callus had the highest zone of inhibition with 17 ± 0.69 mm and 11 ± 0.94 mm. The lowest diameter of zone inhibition of callus extracts was recorded by n-hexane extract at 100 (15 ± 0.46 mm) and 50 mg/ml (09 ± 0.57 mm) respectively. Also, the MIC ranged between 6.25 and 12.50 mg/ml and MFC recorded value of 12.50 mg/ml. Seed methanolic extract had the highest zones of inhibition of 15 ± 0.34 mm and 10 ± 0.62 mm at 100 and 50 mg/ml respectively, while the lowest value (12 ± 0.51 mm and 09 ± 0.23) was recorded in n-hexane at 100 and 50 mg/ml. From the results, both the MIC and MFC of seed extracts ranged from 12.50 to 25.00 mg/ml. Callus extracts showed stronger antifungal activities compared to the seed extracts. Therefore, from the result, Callus extract from B. aegyptiaca can serve as a good source of therapeutic compounds for fungal related disease.

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