655 Analysis of microarray data revealed the long-term effects of neonatal exposure to genistein and bisphenol a on gene expression in mice

2003 ◽  
Vol 144 ◽  
pp. s176
Author(s):  
H. Fukata ◽  
T. Adachi ◽  
M. Komiyama ◽  
K. Sakurai ◽  
C. Mori
Keyword(s):  
Gene Expression ◽  
Bisphenol A ◽  
Microarray Data ◽  
Neonatal Exposure ◽  
Long Term Effects

scholarly journals Long Term Effects of Neonatal Hypoglycemia on Muscarinic Receptor Function in the Cerebellum

2017 ◽  
Vol 2 (1) ◽  
Keyword(s):  
Gene Expression ◽  
Signaling Pathways ◽  
Muscarinic Receptor ◽  
Receptor Subtype ◽  
Receptor Function ◽  
Long Term Effects ◽  
Neonatal Hypoglycemia ◽  
Neonatal Stress ◽  
Old Rats

Neonatal stress conditions like hypoglycemia cause brain damage by affecting various signaling pathways thereby causing long term effects on brain functions. A proper understanding of the signaling pathways affected by this stress will help to devise better neonatal care. The focus of the current study was to evaluate the effect of neonatal hypoglycemic insult on cerebellar metabotropic cholinergic receptor function in one month old rats. The receptor analysis of cholinergic muscarinic receptors were done by radioreceptor assays and gene expression was analysed using Real Time PCR. Neonatal hypoglycemia significantly reduced (p<0.001) the cerebellar muscarinic receptor density with a down regulation (p<0.001) of muscarinic M3 receptor subtype gene expression in one month old rats. Both muscarinic M1 and M2 receptor subtype expression were not significantly altered. The catabolic enzyme in acetyl choline metabolism- acetylcholine esterase – showed a significant (p<0.001) up regulation with no siginificant change in the anabolic enzyme – choline acetyl transferase, signifying a change in the turnover ratio. Targeting these pathways at different levels can be exploited to devise better treatment for neonatal stress management and also for diseases with impaired insulin secretion such as diabetes.

The long-term effects of exposure to ionising radiation on gene expression in mice

2020 ◽  
Vol 821 ◽  
pp. 111723
Author(s):  
Ayman Jafer ◽  
Nicolas Sylvius ◽  
Adeolu B. Adewoye ◽  
Yuri E. Dubrova
Keyword(s):  
Gene Expression ◽  
Ionising Radiation ◽  
Long Term Effects

scholarly journals Developmental conditions modulate DNA methylation at the glucocorticoid receptor gene with cascading effects on expression and corticosterone levels in zebra finches

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Blanca Jimeno ◽  
Michaela Hau ◽  
Elena Gómez-Díaz ◽  
Simon Verhulst
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Glucocorticoid Receptor ◽  
Early Life ◽  
Receptor Gene ◽  
Regulatory Region ◽  
Long Term Effects ◽  
Glucocorticoid Receptor Gene ◽  
Developmental Conditions

Abstract Developmental conditions can impact the adult phenotype via epigenetic changes that modulate gene expression. In mammals, methylation of the glucocorticoid receptor gene Nr3c1 has been implicated as mediator of long-term effects of developmental conditions, but this evidence is limited to humans and rodents, and few studies have simultaneously tested for associations between DNA methylation, gene expression and phenotype. Adverse environmental conditions during early life (large natal brood size) or adulthood (high foraging costs) exert multiple long-term phenotypic effects in zebra finches, and we here test for effects of these manipulations on DNA methylation and expression of the Nr3c1 gene in blood. Having been reared in a large brood induced higher DNA methylation of the Nr3c1 regulatory region in adulthood, and this effect persisted over years. Nr3c1 expression was negatively correlated with methylation at 2 out of 8 CpG sites, and was lower in hard foraging conditions, despite foraging conditions having no effect on Nr3c1 methylation at our target region. Nr3c1 expression also correlated with glucocorticoid traits: higher expression level was associated with lower plasma baseline corticosterone concentrations and enhanced corticosterone reactivity. Our results suggest that methylation of the Nr3c1 regulatory region can contribute to the mechanisms underlying the emergence of long-term effects of developmental conditions in birds, but in our system current adversity dominated over early life experiences with respect to receptor expression.

P.719 Investigation of long-term effects of ouabain administration in mice, and analysis of ouabain-induced gene expression changes in a neuronal culture

2020 ◽  
Vol 40 ◽  
pp. S408-S409
Author(s):  
A. Lopachev ◽  
A.B. Volnova ◽  
Y.A. Timoshina ◽  
R.B. Kazanskaya ◽  
M.A. Lagarkova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Neuronal Culture ◽  
Long Term Effects

scholarly journals Long-term effects of prior diets, dietary transition and pregnancy on adipose gene expression in dairy heifers

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0218723
Author(s):  
Hilde K. L. Wærp ◽  
Sinéad M. Waters ◽  
Matthew S. McCabe ◽  
Paul Cormican ◽  
Ragnar Salte
Keyword(s):  
Gene Expression ◽  
Long Term Effects ◽  
Dairy Heifers ◽  
Dietary Transition

scholarly journals A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

2010 ◽  
Vol 15 (2) ◽  
Author(s):  
Ying Qin ◽  
Ya-Ping Tian
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Real Time ◽  
Microarray Data ◽  
Whole Blood ◽  
Normal Subjects ◽  
Male Rats ◽  
Blood Samples ◽  
Gh Gene

AbstractThe main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in humans. We determined the global gene expression profile of peripheral whole blood from normal adult male rats after long-term GH gene therapy using CapitalBio 27 K Rat Genome Oligo Arrays. Sixty one genes were found to be differentially expressed in GH gene-treated rats 24 weeks after receiving GH gene therapy, at a two-fold higher or lower level compared to the empty vector group (p < 0.05). These genes were mainly associated with angiogenesis, oncogenesis, apoptosis, immune networks, signaling pathways, general metabolism, type I diabetes mellitus, carbon fixation, cell adhesion molecules, and cytokine-cytokine receptor interaction. The results imply that exogenous GH gene expression in normal subjects is likely to induce cellular changes in the metabolism, signal pathways and immunity. A real-time qRT-PCR analysis of a selection of the genes confirmed the microarray data. Eight differently expressed genes were selected as candidate biomarkers from among these 61 genes. These 8 showed five-fold higher or lower expression levels after the GH gene transduction (p < 0.05). They were then validated in real-time PCR experiments using 15 single-treated blood samples and 10 control blood samples. In summary, we detected the gene expression profiles of rat peripheral whole blood after long-term GH gene therapy and screened eight genes as candidate biomarkers based on the microarray data. This will contribute to an increased mechanistic understanding of the effects of chronic GH gene therapy abuse/misuse in normal subjects.

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