Monosaccharide composition analysis of pamiteplase by anion exchange chromatography with pulsed amperometric detection

1999 ◽  
Vol 21 (4) ◽  
pp. 767-774 ◽  
Author(s):  
Hiroyuki Yokota ◽  
Keitarou Mori ◽  
Hideto Yamaguchi ◽  
Hidetoshi Kaniwa ◽  
Norishige Saisho
1999 ◽  
Vol 19 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Hasi Das ◽  
Vani Jayaraman ◽  
Indranil Bhattacharya

Composition analysis of monosaccharides of Sepharose 4B purified NC 92 LPS and the polysaccharides fractions from Sephadex G-50 chromatography was performed by high performance anion exchange chromatography using pulsed amperometric detection. Rhamnose, mannose, galactose and glucose are present in a substantial amount in the purified LPS (Pk I). High molecular weight purified polysaccharides (PS I) obtained after sephadex gel filtration of the purified LPS (Pk I) acid hydrolysate showed an increase in glucose:galactose ratio. This indicates the presence of the peanut root lectin (PRA II) specific sugar in higher proportion on the O-antigen part of the LPS molecule, which may aid in the critical recognition reaction.


1992 ◽  
Vol 233 ◽  
pp. 81-86 ◽  
Author(s):  
Eddy A. Kragten ◽  
Johannis P. Kamerling ◽  
Johannes F.G. Vliegenthart ◽  
Hendrik Botter ◽  
Jan G. Batelaan

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