Analysis by electron microscopy of recombinant plasmids carrying poliovirus type-1 cDNA inserts

ABSTRACT The emergence of circulating vaccine-derived poliovirus (cVDPV) strains in suboptimally vaccinated populations is a serious threat to the global poliovirus eradication. The genetic determinants for the transmissibility phenotype of polioviruses, and in particularly of cVDPV strains, are currently unknown. Here we describe the fecal excretion of wild-type poliovirus, oral polio vaccine, and cVDPV (Hispaniola) strains after intraperitoneal injection in poliovirus receptor-transgenic mice. Both the pattern and the level of fecal excretion of the cVDPV strains resemble those of wild-type poliovirus type 1. In contrast, very little poliovirus was present in the feces after oral polio vaccine administration. This mouse model will be helpful in elucidating the genetic determinants for the high fecal-oral transmission phenotype of cVDPV strains.

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Infectious gastroenteritis in Norfolk Island and recovery of viruses from drinking water

Journal of Hygiene ◽

10.1017/s0022172400060113 ◽

1983 ◽

Vol 91 (1) ◽

pp. 139-146 ◽

Cited By ~ 19

Author(s):

A. M. Murphy ◽

G. S. Grohmann ◽

M. F. H. Sexton

Keyword(s):

Electron Microscopy ◽

Drinking Water ◽

Cell Cultures ◽

Electron Microscopic Examination ◽

Electron Microscopic ◽

Infectious Gastroenteritis ◽

High Incidence ◽

Norfolk Island ◽

High Level

SUMMARYA high incidence of gastroenteritis in both islanders and tourists has been recorded in recent years on Norfolk Island – a popular tourist resort for Australians and New Zealanders. No bacterial cause has been found. However, electron microscopic examination of 28 faecal specimens revealed viruses associated with gastroenteritis in 21 (75%). No viruses were isolated in cell cultures. Bore water is used for drinking purposes on the island and 32 samples from 15 bores were examined for viruses by electron microscopy and culture as well as for bacterial contamination. Seven polioviruses (all type 1 vaccine strain) and adenoviruses 1 and 5 were isolated in cell cultures. In addition one rotavirus, one adenovirus and two small round viruses were detected by electron microscopy. Six of 21 samples tested showed unacceptably high levels of bacteria for drinking water. The deep ground water has apparently become contaminated with sewage effluent and is almost certainly the main cause of the high level of gastroenteritis on the island.

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Molecular epidemiology of wild poliovirus type 1 circulation in West and Central Africa, from 1997 to 1999, using genotyping with a restriction fragment length polymorphism assay

Archives of Virology ◽

10.1007/s00705-007-0001-x ◽

2007 ◽

Vol 153 (3) ◽

pp. 409-416 ◽

Cited By ~ 2

Author(s):

Ionela Gouandjika-Vasilache ◽

Cara C. Burns ◽

Nicksy Gumede ◽

Sophie Guillot ◽

Didier Ménard ◽

...

Keyword(s):

Restriction Fragment Length Polymorphism ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Central Africa ◽

Poliovirus Type ◽

Wild Poliovirus ◽

West And Central Africa ◽

Restriction Fragment Length

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Structure and Polymorphism of the UL6 Portal Protein of Herpes Simplex Virus Type 1

Journal of Virology ◽

10.1128/jvi.78.22.12668-12671.2004 ◽

2004 ◽

Vol 78 (22) ◽

pp. 12668-12671 ◽

Cited By ~ 128

Author(s):

Benes L. Trus ◽

Naiqian Cheng ◽

William W. Newcomb ◽

Fred L. Homa ◽

Jay C. Brown ◽

...

Keyword(s):

Electron Microscopy ◽

Image Analysis ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

Portal Protein ◽

Axial Channel ◽

Simplex Virus

ABSTRACT By electron microscopy and image analysis, we find that baculovirus-expressed UL6 is polymorphic, consisting of rings of 11-, 12-, 13-, and 14-fold symmetry. The 12-mer is likely to be the oligomer incorporated into procapsids: at a resolution of 16 Å, it has an axial channel, peripheral flanges, and fits snugly into a vacant vertex site. Its architecture resembles those of bacteriophage portal/connector proteins.

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