Phylogeographic analysis reveals an ancient East African origin of the human herpes simplexvirus 2 dispersal out-of-Africa.

Human herpes simplex virus 2 (HSV-2) is a globally ubiquitous, slow evolving DNA virus. HSV-2 genomic diversity can be divided into two main groups: an African lineage and worldwide lineage. Competing hypotheses have been put forth to explain the history of HSV-2. HSV-2 may have originated in Africa and then followed the first wave of human migration out of Africa between 50-100 kya. Alternatively, HSV-2 may have migrated out of Africa via the trans-Atlantic slave trade within the last 150-500 years. The lack of HSV-2 genomes from West and Central Africa, combined with a lack of molecular clock signal in HSV-2 has precluded robust testing of these competing hypotheses. Here, we expand the geographic sampling of HSV-2 genomes in order to resolve the geography and timing of divergence events within HSV-2. We analyze 65 newly sequenced HSV-2 genomes collected from primarily West and Central Africa along with 330 previously published genomes sampled over a 47-year period. Evolutionary simulations confirm that the molecular clock in HSV-2 is too slow to be detected using available data. However, phylogeographic analysis indicates that all biologically plausible evolutionary rates would place the ancestor of the worldwide lineage in East Africa, arguing against the trans-Atlantic slave trade as the source of worldwide diversity. The best supported evolutionary rates between 4.2x10-8 and 5.6x10-8 substitutions/site/year suggest a most recent common ancestor for HSV-2 around 90-120 kya and initial dispersal around 21.9-29.3 kya. These dates suggest HSV-2 left Africa during subsequent waves of human migration out of East Africa.

Influence of the Foraging Activity of the Anthophilous Insects on Talinum triangulare (Waterleaf) fructification in Bafut (North West - Cameroon)

Talinum triangulare is an herbaceous succulent plant eaten as a vegetable throughout the tropics including many countries in West and Central Africa and are an essential ingredient in traditional dishes. Experiments were made on the plant to examine the influence of foraging behavior of flowering insect on pollination and yields of this plant species in 2018 and 2019 at Bafut. Observations were made on 1615 to 4055 flowers per treatment. The treatments included unlimited floral access by visitors and bagged flowers to deny all visits. The study focused on the foraging behavior of flowering insects and their pollination activity (fruiting rate). The results show that 14 insect species visited waterleaf flowers and Camponotus flavomarginatus was the most frequent (33.20%). Insects foraged throughout the day light period. Their activity was highest between 10 am and 12 pm. Insect species foraged the flowers for pollen and nectar. The fructification rate of unrestricted floral access was significantly high than that of protected flowers to deny all visits. The maintenance of insect nest close to T. triangulare field is recommended to improve it fruits production.

From the Atlantic Coast to Lake Tanganyika: Gill-Infecting Flatworms of Freshwater Pellonuline Clupeid Fishes in West and Central Africa, with Description of Eleven New Species and Key to Kapentagyrus (Monogenea, Dactylogyridae)

Unlike their marine counterparts, tropical freshwater clupeids receive little scientific attention. However, they sustain important fisheries that may be of (inter)national commercial interest. Africa harbours over 20 freshwater clupeid species within Pellonulini. Recent research suggests their most abundant parasites are gill-infecting monogenean flatworms within Kapentagyrus. After inspecting specimens of 12 freshwater clupeids from West and Central Africa, mainly sourced in biodiversity collections, we propose 11 new species of Kapentagyrus, which we describe using their haptoral and genital morphology. Because of their high morphological similarity, species delineation relies mostly on the morphometrics of anchors and hooks. Specifically, earlier, molecular taxonomic work indicated that the proportion between the length of the anchor roots, and between the hook and anchor length, is diagnostic. On average, about one species of Kapentagyrus exists per pellonuline species, although Pellonula leonensis harbours four species and Microthrissa congica two, while Microthrissa moeruensis and Potamothrissa acutirostris share a gill monogenean species. This study more than quadruples the number of known species of Kapentagyrus, also almost quadrupling the number of pellonuline species of which monogeneans are known. Since members of Kapentagyrus are informative about their hosts’ ecology, evolutionary history, and introduction routes, this enables a parasitological perspective on several data-poor African fisheries.

Training on adequate use of opioid analgesics in West and Central Africa: a neglected step on the way to access to essential medicines?

Alleviating acute and chronic pain is a moral imperative for health professionals and health systems, and it requires adequate access to and use of essential opioid analgesics. However, this is still a neglected issue in global health, with striking inequalities in opioids availability between high and low- and middle-income countries. Countries most affected by lack of access are those with a fragile political situation and weak regulatory and healthcare systems. The main threats to accessibility, availability and affordability are situated at different levels: legislation and policy, financing, knowledge and cultural behavior, erroneous beliefs, and training and education. Among these threats, the lack of (adequate) training and education seems to be a cross-cutting issue. Exploring the current body of knowledge about training and educational activities related to use of opioid analgesics and palliative care, is helpful to understand gaps and to delineate priorities for setting up adequate interventions. When applied to West and Central Africa, this exercise reveals that there is little information (easily) available in the public domain. The African Palliative Care Association (APCA) appears to be the leading provider of capacity building activities in this region for key stakeholders, including national authorities, healthcare professionals and the general population; it is also very active in publishing and communicating about these issues. However, apart from APCA, there is little information on training programs’ contents and long-term outcomes. Furthermore, trainings rarely target important stakeholders such as lawmakers, regulators, supply officers and the lay public (i.e., patients, caregivers, community leaders and members of the society as a whole). Hence, it is urgent to fill the existing gaps in training and educational activities to improve access to essential opioid analgesics in West and Central Africa, involving different stakeholders at the national and regional level. Furthermore, such experiences should be published and made publicly available to allow for mutual learning and further upscale.

French Economic and Monetary Policies in Francophone Africa:

Since granting independence to her former colonies (especially the countries in the West and Central Africa subregions), France has maintained tight economic, political, and to a great extent, social control over their internal and external affairs. These continued ties with France have become the subject of contentious debates (previously considered taboo) among scholars in recent times, evidenced in the development of activism in Africa and continental Europe where the former has been sensitized or radicalized about France’s exploitative approach to economic partnership. The economies of these African nations have suffered stagnation and retrogression in contrast to their non-French-influenced neighbors. This essay employs a literature review to assess the impact of French hegemony over these former colonies, therefore providing a cogent argument for the abolition of the monetary agreement in favor of a local currency, and cessation of political dependencies that also carry a negative stigma. Intellectuals and politicians have argued that the continued use of the CFA franc currency (a relic of colonialism with a different twist) is exploitative; recognized even by French politicians who have appealed to their government to employ moral and ethical considerations to desist from the persistent exploitation of Africa. Social movements have developed today in demanding that African nations still using this currency should withdraw from the agreements due to the severe negative effects on economic development.

From the Atlantic Coast to Lake Tanganyika: Gill-Infecting Flatworms of Freshwater Pellonuline Clupeid Fishes in West and Central Africa, With Description of Eleven New Species and Key to Kapentagyrus (Monogenea, Dactylogyridae)

Unlike their marine counterparts, tropical freshwater clupeids receive little scientific attention. However, they sustain important fisheries that may be of (inter)national commercial interest. Africa harbours over 20 freshwater clupeid species within Pellonulini. Recent research suggests their most abundant parasites are gill-infecting monogenean flatworms within Kapentagyrus. After inspecting specimens of 12 freshwater clupeids from West and Central Africa, mainly sourced in biodiversity collections, we propose 11 new species of Kapentagyrus which we describe using their haptoral and genital morphology. Because of their high morphological similarity, species delineation relies mostly on morphometrics of anchors and hooks. Specifically, earlier, molecular taxonomic work indicated that the proportion between the length of the anchor roots, and between hook and anchor length, are diagnostic. On average, about one species of Kapentagyrus exists per pellonuline species, although Pellonula leonensis harbours four species and Microthrissa congica two, while Microthrissa moeruensis and Potamothrissa acutirostris share a gill monogenean species. This study more than quadruples the number of known species of Kapentagyrus, also almost quadrupling the number of pellonuline species of which monogeneans are known. Since members of Kapentagyrus are informative about their hosts’ ecology, evolutionary history, and introduction routes, this enables a parasitological perspective on several data-poor African fisheries.

Assessment of genetic variability among accessions of okra (Abelmoschus esculentus L. Moench)

Okra yields are low in West and Central Africa and factors including narrow genetic base of available germplasm have been implicated. An assessment of genetic variability among germplasm from various sources and knowledge of trait contributions to these variations is crucial to the success of okra breeding program. Eighteen okra accessions were evaluated during the 2020 cropping season in Nigeria to assess the genotypes for genetic diversity, group the accessions into clusters and identify traits that chiefly accounted for the variations among the genotypes. Data obtained were subjected to analysis of variance (ANOVA), metroglyph and principal component analyses (PCA). ANOVA revealed significant accession mean squares for majority of the measured traits. Metroglyph analysis grouped the accessions into four clusters with members of Cluster IV generally high-yielding, early-maturing and short genotypes. NGB00353 and NGB00356 that were among genotypes with high total index scores were members of Cluster IV. The first four principal components (PCs) accounted for 80% of the total observed variation. PC1 with the highest discriminatory power was loaded with days to budding, internode length, pod yield.plot−1 and the number of pods.plant−1. The variation within and between clusters could be explored in okra breeding program.