Controls on the origin and evolution of deep-ocean trench-axial channels

The type and volume of sediment entering subduction zones affects the style of plate-boundary deformation and thus sedimentary and tectonic cycles. Because submarine channels significantly increase the transport efficiency of turbidity currents, their presence or absence in subduction trenches is a primary control on trench fill. To date, comprehensive architectural characterization of trench-axial channels has not been possible, undermining efforts to identify the factors controlling their initiation and evolution. Here, we describe the evolution of the Hikurangi Channel, which traverses the Hikurangi Trench, offshore New Zealand. Analysis of two- and three-dimensional seismic data reveals that the channel was present only during the last ~3.5 m.y. of the ~27 m.y. of the trench’s existence; its inception and propagation resulted from increased sediment supply to the trench following amplified hinterland exhumation. To test if the controls on the evolution of the Hikurangi Channel are universal, multivariate statistical analysis of the geomorphology of subduction trenches globally is used to investigate the formative conditions of axial channels in modern trenches. Terrigenous sediment supply and thickness of sediment cover in a trench are the dominant controls; subsidiary factors such as trench length and rugosity also contribute to the conditions necessary for trench-axial channel development. Axial channels regulate sediment distribution in trenches, and this varies temporally and spatially as a channel propagates along a trench. The presence of a trench-axial channel affects plate-boundary mechanics and has implications for the style of subduction-margin deformation.

Pengaruh Kombinasi Pemangkasan Ujung Magnet Permanen dan Axial Channel pada inti rotor terhadap Torka Cogging pada Generator Tipe Fractional Slot Number

This paper is about to discuss the effect of combining a magnetic shaping technique with an axial channel in the rotor core to reduce the cogging torque of a permanent magnet synchronous generator. Computation process is performed by using the optimization response surface method. In this case, this research is done by employing two types of axial channel systems, namely circular and hexagonal. The axial channel area at the core of the engine rotor is 0.000279683 m2. Determination of magnetic shaping was carried out with an angle of 10 and a surface angle of 530. The effect of the combination of the cogging torque reduction technique with magnetic shaping and axal channel was analyzed by numerical method based on the finite element method (FEMM). Based on the analysis, it is found that the combination shows a decrease in cogging torque by 98% when compared with the cogging torque in the initial design (initial structure). Another advantage of the combination of the two cogging torque reduction techniques is that there is no significant increase in the magnetic flux density of the engine core. It can be said that the combination of the cogging torque reduction technique and the axial channel at the core of the engine rotor can significantly reduce the cogging torque.

Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates

When ribosomes fail to complete normal translation, all cells have mechanisms to ensure degradation of the resulting partial proteins to safeguard proteome integrity. In Escherichia coli and other eubacteria, the tmRNA system rescues stalled ribosomes and adds an ssrA tag or degron to the C-terminus of the incomplete protein, which directs degradation by the AAA+ ClpXP protease. Here, we present cryo-EM structures of ClpXP bound to the ssrA degron. C-terminal residues of the ssrA degron initially bind in the top of an otherwise closed ClpX axial channel and subsequently move deeper into an open channel. For short-degron protein substrates, we show that unfolding can occur directly from the initial closed-channel complex. For longer degron substrates, our studies illuminate how ClpXP transitions from specific recognition into a nonspecific unfolding and translocation machine. Many AAA+ proteases and protein-remodeling motors are likely to employ similar multistep recognition and engagement strategies.

Structural basis of ClpXP recognition and unfolding of ssrA-tagged substrates

ABSTRACTWhen ribosomes fail to complete normal translation, all cells have mechanisms to ensure degradation of the resulting partial proteins to safeguard proteome integrity. In E. coli and other eubacteria, the tmRNA system rescues stalled ribosomes and adds an ssrA tag or degron to the C-terminus of the incomplete protein, which directs degradation by the AAA+ ClpXP protease. Here, we present cryo-EM structures of ClpXP bound to the ssrA degron. C-terminal residues of the ssrA degron initially bind in the top of an otherwise closed ClpX axial channel and subsequently move deeper into an open channel. For short-degron protein substrates, we show that unfolding can occur directly from the initial closed-channel complex. For longer-degron substrates, our studies illuminate how ClpXP transitions from specific recognition into a nonspecific unfolding and translocation machine. Many AAA+ proteases and protein-remodeling motors are likely to employ similar multistep recognition and engagement strategies.

Анизотропия плазмы при извлечении из нее заряженных частиц

The anisotropic perturbation of a hollow-cathode reflex discharge plasma when electrons are extracted from it through an axial channel in the cathode-reflector is experimentally investigated. The expansion of the extracted electrons energy spectrum in the direction of extraction was observed as the external accelerating electric field increased. The energy spectrum expansion is associated with the plasma electrons directional movement towards the emitting surface of plasma. The axial gradients of the concentration and the electric potential are assumed to be the reasons for the directional movement of electrons.

Numerical Modeling of a Wave Turbine and Estimation of Shaft Work

Wave rotors are periodic-flow devices that provide dynamic pressure exchange and efficient energy transfer through internal pressure waves generated due to fast opening and closing of ports. Wave turbines are wave rotors with curved channels that can produce shaft work through change of angular momentum from inlet to exit. In the present work, conservation equations with averaging in the transverse directions are derived for wave turbines, and quasi-one-dimensional model for axial-channel non-steady flow is extended to account for blade curvature effects. The importance of inlet incidence is explained and the duct angle is optimized to minimize incidence loss for a particular boundary condition. Two different techniques are presented for estimating the work transfer between the gas and rotor due to flow turning, based on conservation of angular momentum and of energy. The use of two different methods to estimate the shaft work provides confidence in reporting of work output and confirms internal consistency of the model while it awaits experimental data for validation. The extended wave turbine model is used to simulate the flow in a three-port wave rotor. The work output is calculated for blades with varying curvature, including the straight axial channel as a reference case. The dimensional shaft work is reported for the idealized situation where all loss-generating mechanisms except flow incidence are absent, thus excluding leakage, heat transfer, friction, port opening time, and windage losses. The model developed in the current work can be used to determine the optimal wave turbine designs for experimental investment.

Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model

During replication initiation, the core component of the helicase—the Mcm2-7 hexamer—is loaded on origin DNA as a double hexamer (DH). The two ring-shaped hexamers are staggered, leading to a kinked axial channel. How the origin DNA interacts with the axial channel is not understood, but the interaction could provide key insights into Mcm2-7 function and regulation. Here, we report the cryo-EM structure of the Mcm2-7 DH on dsDNA and show that the DNA is zigzagged inside the central channel. Several of the Mcm subunit DNA-binding loops, such as the oligosaccharide–oligonucleotide loops, helix 2 insertion loops, and presensor 1 (PS1) loops, are well defined, and many of them interact extensively with the DNA. The PS1 loops of Mcm 3, 4, 6, and 7, but not 2 and 5, engage the lagging strand with an approximate step size of one base per subunit. Staggered coupling of the two opposing hexamers positions the DNA right in front of the two Mcm2–Mcm5 gates, with each strand being pressed against one gate. The architecture suggests that lagging-strand extrusion initiates in the middle of the DH that is composed of the zinc finger domains of both hexamers. To convert the Mcm2-7 DH structure into the Mcm2-7 hexamer structure found in the active helicase, the N-tier ring of the Mcm2-7 hexamer in the DH-dsDNA needs to tilt and shift laterally. We suggest that these N-tier ring movements cause the DNA strand separation and lagging-strand extrusion.