An in vivo—In vitro study of the use of a human skin equivalent for irritancy screening of fatty acids

We have previously reported morphological correlates of sulfur mustard (HD) toxicity in several model systems: the human skin grafted athymic nude mouse; the hairless guinea pig; and human cells in culture. We are now describing HD effects in a human skin equivalent, TESTSKIN®, and comparing these effects with those already reported for animal models and cells in culture. The human skin equivalent (HSE) is used here as an organotypic in vitro model system to bridge the knowledge gap between HD effects in monotypic cells in culture and animal in vivo effects. Additionally, HSE allowed study of HD toxicity which circumvented the concern of using human biopsied tissue.

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Assessment of penetration of quantum dots through in vitro and in vivo human skin using the human skin equivalent model and the tape stripping method

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2010.03.032 ◽

2010 ◽

Vol 394 (3) ◽

pp. 612-615 ◽

Cited By ~ 48

Author(s):

Sang Hoon Jeong ◽

Jae Hwan Kim ◽

Sang Min Yi ◽

Jung Pyo Lee ◽

Jin Ho Kim ◽

...

Keyword(s):

Quantum Dots ◽

Human Skin ◽

Skin Equivalent ◽

Equivalent Model ◽

Tape Stripping ◽

Stripping Method ◽

Human Skin Equivalent Model ◽

Human Skin Equivalent

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In vitro study of the bioavailability of fatty acids in cow milk containing conjugated linoleic acids produced in vivo or added in synthetic form

International Dairy Journal ◽

10.1016/j.idairyj.2014.01.009 ◽

2014 ◽

Vol 36 (2) ◽

pp. 95-100 ◽

Cited By ~ 5

Author(s):

Hajer Jedidi ◽

Ehab Kheadr ◽

Fleur Gagnon ◽

Marie-Rose Van Calsteren ◽

Edward Farnworth ◽

...

Keyword(s):

Fatty Acids ◽

In Vitro Study ◽

Vitro Study ◽

Cow Milk ◽

Conjugated Linoleic Acids ◽

Synthetic Form

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Desmosomal distribution in the epidermis of a non-contracting human skin equivalent

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124884 ◽

1992 ◽

Vol 50 (1) ◽

pp. 896-897

Author(s):

L.X. Oakford ◽

S.D. Dimitrijevich ◽

R. Gracy

Keyword(s):

Human Skin ◽

Basal Layer ◽

Skin Equivalent ◽

Tissue Component ◽

Intact Skin ◽

Similar Sequence ◽

Sequence Of Events ◽

Suprabasal Keratinocytes ◽

Human Skin Equivalent

In intact skin the epidermal layer is a dynamic tissue component which is maintained by a basal layer of mitotically active cells. The protective upper epidermis, the stratum corneum, is generated by differentiation of the suprabasal keratinocytes which eventually desquamate as anuclear comeocytes. A similar sequence of events is observed in vitro in the non-contracting human skin equivalent (HSE) which was developed in this lab (1). As a part of the definition process for this model of living skin we are examining its ultrastructural features. Since desmosomes are important in maintaining cell-cell interactions in stratified epithelia their distribution in HSE was examined.

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