Effect of cigarette smoke on oral peroxidase activity in human saliva: role of hydrogen cyanide

2003 ◽  
Author(s):  
I Klein
Keyword(s):  
Cigarette Smoke ◽  
Peroxidase Activity ◽  
Hydrogen Cyanide ◽  
Human Saliva

Effect of cigarette smoke on oral peroxidase activity in human saliva: role of hydrogen cyanide

2003 ◽  
Vol 35 (11) ◽  
pp. 1448-1452 ◽  
Author(s):  
Ifat Klein ◽  
Rafael M Nagler ◽  
Ruth Toffler ◽  
Albert van Der Vliet ◽  
Abraham Z Reznick
Keyword(s):  
Cigarette Smoke ◽  
Peroxidase Activity ◽  
Hydrogen Cyanide ◽  
Human Saliva

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

scholarly journals Pivotal role of MUC1 glycosylation by cigarette smoke in modulating disruption of airway adherens junctions in vitro

2014 ◽  
Vol 234 (1) ◽  
pp. 60-73 ◽  
Author(s):  
Lili Zhang ◽  
Marianne Gallup ◽  
Lorna Zlock ◽  
Yu Ting Feeling Chen ◽  
Walter E Finkbeiner ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Adherens Junctions ◽  
Pivotal Role

Functional porous carboxymethylcellulose microspheres – preparation and reduction of hydrogen cyanide yield in cigarette smoke

2015 ◽  
Vol 19 (sup6) ◽  
pp. S6-21-S6-25
Author(s):  
P. J. Sun ◽  
S. Yang ◽  
X. H. Sun ◽  
Y. Z. Jia ◽  
Y. P. Wang ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Hydrogen Cyanide

scholarly journals Role of thromboxane-A2 and cholinergic mechanisms in bronchoconstriction induced by cigarette smoke in guinea-pigs

1996 ◽  
Vol 9 (12) ◽  
pp. 2468-2473 ◽  
Author(s):  
K. Matsumoto ◽  
H. A Izawa ◽  
H. Inoue ◽  
S. Takata ◽  
M. Shigyo ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Guinea Pigs ◽  
Cholinergic Mechanisms

scholarly journals Critical role of fractalkine (CX3CL1) in cigarette smoke-induced mononuclear cell adhesion to the arterial endothelium

Thorax ◽  
2012 ◽  
Vol 68 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Cristina Rius ◽  
Chantal Company ◽  
Laura Piqueras ◽  
Jose Miguel Cerdá-Nicolás ◽  
Cruz González ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cigarette Smoke ◽  
Mononuclear Cell ◽  
Critical Role ◽  
Arterial Endothelium

scholarly journals Will the Bacteria Survive in the CeO2 Nanozyme-H2O2 System?

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3747
Author(s):  
Weisheng Zhu ◽  
Luyao Wang ◽  
Qisi Li ◽  
Lizhi Jiao ◽  
Xiaokan Yu ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Biomedical Applications ◽  
Antibacterial Effect ◽  
Ros Production ◽  
Ros Scavenging ◽  
Ros Scavenger ◽  
Oxide Nanoparticle ◽  
H2o2 System ◽  
Bacterial Disinfection

As one of the nanostructures with enzyme-like activity, nanozymes have recently attracted extensive attention for their biomedical applications, especially for bacterial disinfection treatment. Nanozymes with high peroxidase activity are considered to be excellent candidates for building bacterial disinfection systems (nanozyme-H2O2), in which the nanozyme will promote the generation of ROS to kill bacteria based on the decomposition of H2O2. According to this criterion, a cerium oxide nanoparticle (Nanoceria, CeO2, a classical nanozyme with high peroxidase activity)-based nanozyme-H2O2 system would be very efficient for bacterial disinfection. However, CeO2 is a nanozyme with multiple enzyme-like activities. In addition to high peroxidase activity, CeO2 nanozymes also possess high superoxide dismutase activity and antioxidant activity, which can act as a ROS scavenger. Considering the fact that CeO2 nanozymes have both the activity to promote ROS production and the opposite activity for ROS scavenging, it is worth exploring which activity will play the dominating role in the CeO2-H2O2 system, as well as whether it will protect bacteria or produce an antibacterial effect. In this work, we focused on this discussion to unveil the role of CeO2 in the CeO2-H2O2 system, so that it can provide valuable knowledge for the design of a nanozyme-H2O2-based antibacterial system.

Abstract 644: Role of Micro RNA LET-7F in Cigarette Smoke-Induced Impairment of Neovascularization

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Wahiba Dhahri ◽  
Sylvie Dussault ◽  
Paola Haddad ◽  
Julie Turgeon ◽  
Sophie Tremblay ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Vascular Endothelial Cells ◽  
Capillary Density ◽  
Tube Formation ◽  
Micro Rna ◽  
Cellular Migration ◽  
Mirna Array ◽  
Inhibition Of Angiogenesis

Background: Exposure to cigarette smoke is associated with impaired neovascularization in response to ischemia. The precise mechanisms involved in that process remain to be determined. Micro RNA (miR) are emerging as key regulators of several physiological processes, including angiogenesis. Here we investigated the potential role of miRs for the modulation of neovascularization in the context of cigarette smoking. Methods and Results: Human Umbilical Vascular Endothelial Cells (HUVECs) were exposed or not to cigarette smoke extracts (CSE). Using Affimetrix GeneChip miRNA array analysis, we found that the pro-angiogenic miR let-7f was downregulated by 40% in HUVECs exposed to CSE. Using an inhibitor of let-7f, we demonstrated reduced migration and tube formation in HUVECs, reproducing the phenotype induced by CSE. A let-7f mimic could rescue cellular migration and tube formation in HUVECs exposed to CSE. Moreover, the expression of let-7f is significantly reduced in the ischemic muscles of mice exposed to cigarette smoke (CS). In vivo, hindlimb ischemia was surgically provoked by femoral artery removal to mice exposed (SMK) or not to CS for two weeks with a local injection of a control or a let-7f mimic. Let-7f mimic could rescue blood flow recuperation and capillary density in ischemic muscles 21 days post-ischemia associated with improved mobility. We found that CS was associated with reduced number of endothelial progenitor cells (EPCs) and impairment of angiogenic activities. Importantly, let-7f mimic rescued EPC number and EPC functional activities in SMK group. TGF-β-RI and HIF1AN are predicted to be targeted by let-7f and both are increased in SMK mice, whereas the expression of HIF-1a and VEGF are reduced in these mice. Interestingly, SMK mice injected with a let-7f mimic have decreased muscle expression of TGF-β-RI and HIF1AN associated with normalized HIF-1 and VEGF expression. Conclusion: Our results suggest that a reduction in the expression of let-7f could be involved in the cigarette smoke-induced inhibition of angiogenesis through modulation of TGF-β-RI and HIF1AN. Overexpression of let-7f using a miR mimic could constitute a novel therapeutic strategy to improve ischemia-induced neovascularization in pathological conditions.

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