Effects of below-ground herbivory by Diabrotica virgifera virgifera (Coleoptera) on biomass allocation and carbohydrate storage of maize

1998 ◽  
Vol 7 (3) ◽  
pp. 213-218 ◽  
Author(s):  
James P Dunn ◽  
Keri Frommelt
Author(s):  

Abstract A new distribution map is provided for Diabrotica virgifera virgifera LeConte. Coleoptera: Chrysomelidae. Hosts: maize (Zea mays). Information is given on the geographical distribution in Europe (Albania, Austria, Belarus, Belgium, Bosnia-Hercegovina, Bulgaria, Croatia, Czech Republic, France, Germany, Greece, Hungary, Italy, Montenegro, Netherlands, Poland, Romania, Russia, Serbia, Slovakia, Switzerland, UK (England and Wales), Ukraine, Canada (Ontario, Quebec), Mexico, USA (Alabama, Arizona, Colorado, Connecticut, Delaware, Georgia, Idaho, Illinois, Indiana, Iowa, Kansas, Kentucky, Louisiana, Maine, Massachusetts, Michigan, Minnesota, Missouri, Montana, Nebraska, New Hampshire, New Jersey, New Mexico, New York, North Carolina, North Dakota, Ohio, Oklahoma, Pennsylvania, Rhode Island, South Dakota, Texas, Utah, Virginia, Washington, West Virginia, Wisconsin, Wyoming)), Central America & Caribbean (Costa Rica, Guatemala, Nicaragua).


2020 ◽  
Author(s):  
Zixiao Zhao ◽  
Christine G. Elsik ◽  
Bruce E. Hibbard ◽  
Kent S. Shelby

AbstractBackgroundAlternative splicing is one of the major mechanisms that increases transcriptome diversity in eukaryotes, including insect species that have gained resistance to pesticides and Bt toxins. In western corn rootworm (Diabrotica virgifera virgifera LeConte), neither alternative splicing nor its role in resistance to Bt toxins has been studied.ResultsTo investigate the mechanisms of Bt resistance we carried out single-molecule real-time (SMRT) transcript sequencing and Iso-seq analysis on resistant, eCry3.1Ab-selected and susceptible, unselected, western corn rootworm neonate midguts which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. We present transcriptome-wide alternative splicing patterns of western corn rootworm midgut in response to feeding on eCry3.1Ab-expressing corn using a comprehensive approach that combines both RNA-seq and SMRT transcript sequencing techniques. We found that 67.73% of multi-exon genes are alternatively spliced, which is consistent with the high transposable element content of the genome. One of the alternative splicing events we identified was a novel peritrophic matrix protein with two alternative splicing isoforms. Analysis of differential exon usage between resistant and susceptible colonies showed that in eCry3.1Ab-resistant western corn rootworm, expression of one isoform was significantly higher than in the susceptible colony, while no significant differences between colonies were observed with the other isoform.ConclusionOur results provide the first survey of alternative splicing in western corn rootworm and suggest that the observed alternatively spliced isoforms of peritrophic matrix protein may be associated with eCry3.1Ab resistance in western corn rootworm.


Author(s):  
Cornelia CIOBANU ◽  
Cornel DOMUŢA ◽  
Gheorghe CIOBANU ◽  
Maria ŞANDOR ◽  
Alina Dora SAMUEL ◽  
...  

The paper based on the researches carried out during 2005 – 2008 in the long term trial placed in 1990 on the preluvosoil from Oradea. The monoculture of maize, maize – wheat and maize – soybean – wheat crop rotations were studied in unirrigated and irrigated conditions. The crop rotatin of six years (oat + clover – clover – maize – wheat – maize - sunflower) was studied in unirrigated conditions. The researches emphasized that the monoculture is the most responsible for the pest multiplication. In maize monoculture the larvae number on the roots was ranged between 4.91-8.23 and root attack degree in IOWA scale (with marks from 1 to 6 in which maximum attack is 6), had values between 3.84 and 5.62 and the frequent of attacked plants with the symptom “goose neck” ranged between 16,4% and 31.2% and larval aggressiveness being higher in the case of favorable soil moisture in irrigated condition. The maize rotation with other plants interrupts the biologic pest cycle. The results obtained emphasize that later maize sowing alongside by the utilizing of lower plant thickness level contribute to prevention of pest multiplication, while earlier sowing at high thickness favor the larvae developing.


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