Design and synthesis of functionalized glycomers as non-peptidic ligands for SH2 binding and as inhibitors of A-431 human epidermoid and HT-29 colon carcinoma cell lines

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.

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Activities of purine-metabolizing enzymes in human colon carcinoma cell lines and xenograft tumors

Biochemical Pharmacology ◽

10.1016/0006-2952(81)90167-2 ◽

1981 ◽

Vol 30 (7) ◽

pp. 793-798 ◽

Cited By ~ 11

Author(s):

Gerald W. Crabtree ◽

Daniel L. Dexter ◽

Johanna D. Stoeckler ◽

Todd M. Savarese ◽

Lucy Y. Ghoda ◽

...

Keyword(s):

Colon Carcinoma ◽

Cell Lines ◽

Carcinoma Cell ◽

Human Colon ◽

Human Colon Carcinoma Cell ◽

Metabolizing Enzymes ◽

Carcinoma Cell Lines ◽

Human Colon Carcinoma

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Protein kinase C and casein kinase II activities in two human colon carcinoma cell lines, HT-29 and CaCo-2: Possible correlation with differentiation

Bioscience Reports ◽

10.1007/bf01117245 ◽

1990 ◽

Vol 10 (3) ◽

pp. 293-299 ◽

Cited By ~ 6

Author(s):

Ewa Rydell ◽

Karl-Eric Magnusson ◽

Anita Sjö ◽

Krister Axelsson

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Colon Carcinoma ◽

Carcinoma Cell ◽

Human Colon ◽

Human Colon Carcinoma Cell ◽

Carcinoma Cell Lines ◽

Kinase C ◽

Human Colon Carcinoma ◽

Ht 29

Protein kinase C (PK-C) and casein kinase II (CK-II) activities were studied in two human colon carcinoma cell lines (HT-29 and CaCO-2) undergoing differentiation in vitro resulting, in small-intestine-like cells. CaCo-2 cells, when grown under standard conditions, appear to undergo spontaneous differentiation. In these cells PK-C and CK-II activities were determined on day 5, 10 and 15. No significant differences in activities were seen either in PK-C or CK-II activity. HT-29 cells, when grown in glucose-free medium can be stimulated to undergo differentiation which is completed within 20 days. PK-C and CK-II activities were determined after 5, 10, 15, 20 and 25 days, respectively. PK-C activity rose from 7.9±3.5 pmole32P/mg protein/min at day 5 to 37.5±14.8 pmole32P/mg protein/min at day 20. After 25 days the activity was reduced to 20.0±7.8 pmole32P/mg protein/min. CK-II activity did not change significantly during day 5 to 20, but on day 25 there was a significant decrease in CK-II activity from 94.9±6.4 pmole32P/mg protein/min (day 20) to 62.6±3.9 pmole32P/mg protein/min (day 25) p=0.003. The results in this study indicate a role for PK-C and CK-II in cell growth and differentiation.

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