Integrated analysis reveals an association between the rhizosphere microbiome and root rot of arecanut palm

Pedosphere ◽  
2021 ◽  
Vol 31 (5) ◽  
pp. 725-735
Author(s):  
Hong LI ◽  
Xiang MA ◽  
Yanqiong TANG ◽  
Chengliang YAN ◽  
Xinwen HU ◽  
...  
Keyword(s):  
Root Rot ◽  
Integrated Analysis ◽  
Rhizosphere Microbiome ◽  
Arecanut Palm

scholarly journals Phytophthora Root Rot Modifies the Composition of the Avocado Rhizosphere Microbiome and Increases the Abundance of Opportunistic Fungal Pathogens

2021 ◽  
Vol 11 ◽  
Author(s):  
Itzel A. Solís-García ◽  
Oscar Ceballos-Luna ◽  
Elvis Marian Cortazar-Murillo ◽  
Damaris Desgarennes ◽  
Edith Garay-Serrano ◽  
...  
Keyword(s):  
Root Rot ◽  
Fungal Pathogens ◽  
Microbial Consortia ◽  
Plant Growth Promoting Bacteria ◽  
Its Sequencing ◽  
Phytophthora Root Rot ◽  
Rhizosphere Microbiome ◽  
Plant Growth Promoting ◽  
And Function ◽  
Induced Changes

The structure and function of rhizosphere microbial communities are affected by the plant health status. In this study, we investigated the effect of root rot on the avocado rhizosphere microbiome, using 16S rDNA and ITS sequencing. Furthermore, we isolated potential fungal pathogens associated with root rot symptoms and assessed their pathogenic activity on avocado. We found that root rot did not affect species richness, diversity or community structure, but induced changes in the relative abundance of several microbial taxa. Root rot increased the proportion of Pseudomonadales and Burkholderiales in the rhizosphere but reduced that of Actinobacteria, Bacillus spp. and Rhizobiales. An increase in putative opportunistic fungal pathogens was also detected in the roots of symptomatic trees; the potential pathogenicity of Mortierella sp., Fusarium spp., Lasiodiplodia sp. and Scytalidium sp., is reported for the first time for the State of Veracruz, Mexico. Root rot also potentially modified the predicted functions carried out by rhizobacteria, reducing the proportion of categories linked with the lipid and amino-acid metabolisms whilst promoting those associated with quorum sensing, virulence, and antibiotic resistance. Altogether, our results could help identifying microbial taxa associated to the disease causal agents and direct the selection of plant growth-promoting bacteria for the development of biocontrol microbial consortia.

scholarly journals Transcriptome and metabolite profiling reveals the effects of Funneliformis mosseae on the roots of continuously cropped soybeans

2020 ◽  
Author(s):  
Cheng-Cheng Lu ◽  
Na Guo ◽  
Chao Yang ◽  
Hai-Bing Sun ◽  
Baiyan Cai
Keyword(s):  
Root Rot ◽  
Mycorrhizal Fungi ◽  
Plant Stress ◽  
Arbuscular Mycorrhizal ◽  
Ultra Performance Liquid Chromatography ◽  
Integrated Analysis ◽  
Continuous Cropping ◽  
Soybean Root ◽  
Disease Resistant ◽  
Coa Reductase

Abstract Background Arbuscular mycorrhizal fungi are the most widely distributed mycorrhizal fungi, which can form mycorrhizal symbionts with plant roots and enhance plant stress resistance by regulating host metabolic activities. In this paper, the RNA sequencing and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) technologies were used to study the transcriptome and metabolite profiles of the roots of continuously cropped soybeans that were infected with F. mosseae and F. oxysporum. The objective was to explore the effects of F. mosseae treatment on soybean root rot infected with F. oxysporum. Results According to the transcriptome profiles, 24285 differentially expressed genes (DEGs) were identified, and the expression of genes encoding phenylalanine ammonia lyase (PAL), trans-cinnamate monooxygenase (CYP73A), cinnamyl-CoA reductase (CCR), chalcone isomerase (CHI) and coffee-coenzyme o-methyltransferase were upregulated after being infected with F. oxysporum; these changes were key to the induction of the soybean’s defence response. The metabolite results showed that daidzein and 7,4-dihydroxy, 6-methoxy isoflavone (glycine), which are involved in the isoflavone metabolic pathway, were upregulated after the roots were inoculated with F. mosseae. In addition, a substantial alteration in the abundance of amino acids, phenolic and terpene metabolites all led to the synthesis of defence compounds. An integrated analysis of the metabolic and transcriptomic data revealed that substantial alterations in the abundance of most of the intermediate metabolites and enzymes changed substantially under pathogen infection. These changes included the isoflavonoid biosynthesis pathway, which suggests that isoflavonoid biosynthesis plays an important role in the soybean root response. Conclusion The results showed that F. mosseae could alleviate the root rot caused by continuous cropping. The increased activity of some disease-resistant genes and disease-resistant metabolites may partly account for the ability of the plants to resist diseases. This study provides new insights into the molecular mechanism by which AMF alleviates soybean root rot, which is important in agriculture.

scholarly journals Transcriptome and metabolite profiling reveals the effects of Funneliformis mosseae on the roots of continuously cropped soybeans

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Cheng-Cheng Lu ◽  
Na Guo ◽  
Chao Yang ◽  
Hai-Bing Sun ◽  
Bai-Yan Cai
Keyword(s):  
Root Rot ◽  
Mycorrhizal Fungi ◽  
Plant Stress ◽  
Arbuscular Mycorrhizal ◽  
Ultra Performance Liquid Chromatography ◽  
Integrated Analysis ◽  
Continuous Cropping ◽  
Soybean Root ◽  
Disease Resistant ◽  
Coa Reductase

Abstract Background Arbuscular mycorrhizal fungi are the most widely distributed mycorrhizal fungi, which can form mycorrhizal symbionts with plant roots and enhance plant stress resistance by regulating host metabolic activities. In this paper, the RNA sequencing and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) technologies were used to study the transcriptome and metabolite profiles of the roots of continuously cropped soybeans that were infected with F. mosseae and F. oxysporum. The objective was to explore the effects of F. mosseae treatment on soybean root rot infected with F. oxysporum. Results According to the transcriptome profiles, 24,285 differentially expressed genes (DEGs) were identified, and the expression of genes encoding phenylalanine ammonia lyase (PAL), trans-cinnamate monooxygenase (CYP73A), cinnamyl-CoA reductase (CCR), chalcone isomerase (CHI) and coffee-coenzyme o-methyltransferase were upregulated after being infected with F. oxysporum; these changes were key to the induction of the soybean’s defence response. The metabolite results showed that daidzein and 7,4-dihydroxy, 6-methoxy isoflavone (glycine), which are involved in the isoflavone metabolic pathway, were upregulated after the roots were inoculated with F. mosseae. In addition, a substantial alteration in the abundance of amino acids, phenolic and terpene metabolites all led to the synthesis of defence compounds. An integrated analysis of the metabolic and transcriptomic data revealed that substantial alterations in the abundance of most of the intermediate metabolites and enzymes changed substantially under pathogen infection. These changes included the isoflavonoid biosynthesis pathway, which suggests that isoflavonoid biosynthesis plays an important role in the soybean root response. Conclusion The results showed that F. mosseae could alleviate the root rot caused by continuous cropping. The increased activity of some disease-resistant genes and disease-resistant metabolites may partly account for the ability of the plants to resist diseases. This study provides new insights into the molecular mechanism by which AMF alleviates soybean root rot, which is important in agriculture.

Integrated Analysis of Low-Level Turbulence around Airport Using Meteorological Model and LES

2013 ◽  
Vol 61 (6) ◽  
pp. 159-166
Author(s):  
Ryota KIKUCHI ◽  
Takashi MISAKA ◽  
Shigeru OBAYASHI ◽  
Tomoo USHIO ◽  
Shigeharu SHIMAMURA ◽  
...  
Keyword(s):  
Integrated Analysis ◽  
Meteorological Model ◽  
Low Level

Infections of bean plant and field soil are linked to region, root rot pathogen, and agro-ecosystem

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
B. Naseri ◽  
M. Gheitury ◽  
M. Veisi
Keyword(s):  
Root Rot ◽  
Multivariate Analyses ◽  
Control Method ◽  
Control Strategies ◽  
Fusarium Species ◽  
Macrophomina Phaseolina ◽  
Soil Variables ◽  
Plant Infection ◽  
Pathogen Prevalence ◽  
Soil Infestation

SummaryUnderstanding pathogen-agrosystem interaction is particularly essential when applying a control method to minimize pathogen prevalence prior to plant infection. To meet this requirement, frequency of major root rot pathogens isolated from bean root and seed, and their soil populations were examined in farmers’ fields. Multivariate analyses evidenced more frequent isolations of Fusarium solani and Rhizoctonia solani from root and seed compared to Macrophomina phaseolina and Fusarium oxysporum. Two Fusarium species had denser soil populations than R. solani and M. phaseolina. More frequent isolations of pathogens were detected in root and seed collected from Abhar and Khodabandeh compared to Kheirabad region. Agronomic and soil variables corresponded less closely to root infections compared to soil infestation and seed infections. Bean market class, herbicide application, and planting depth were linked to root, seed and soil infestations. Such information provides a basis for increased confidence in choosing appropriate control strategies for a pathogen and region in sustainable agriculture.

'Shinano Hope', a Fusarium Root Rot-Resistant Lettuce

2004 ◽  
Vol 73 (5) ◽  
pp. 429-434 ◽  
Author(s):  
Nobuaki Tsuchiya ◽  
Kiyoshi Yoshida ◽  
Tomita Usui ◽  
Motohisa Tsukada
Keyword(s):  
Root Rot ◽  
Fusarium Root Rot
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