Improved inter-instrument consistency and high precision for cho cell bioprocessing using the Cellaca™ MX high-throughput cell counter

While crucial for force spectroscopists and microbiologists, three-dimensional (3D) particle tracking suffers from either poor precision, complex calibration, or the need of expensive hardware, preventing its massive adoption. We introduce a new technique, based on a simple piece of cardboard inserted in the objective focal plane, that enables simple 3D tracking of dilute microparticles while offering subnanometer frame-to-frame precision in all directions. Its linearity alleviates calibration procedures, while the interferometric pattern enhances precision. We illustrate its utility in single-molecule force spectroscopy and single-algae motility analysis. As with any technique based on back focal plane engineering, it may be directly embedded in a commercial objective, providing a means to convert any preexisting optical setup in a 3D tracking system. Thanks to its precision, its simplicity, and its versatility, we envision that the technique has the potential to enhance the spreading of high-precision and high-throughput 3D tracking.

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Novel Electrochemiluminescent Assays for Drug Discovery

JALA Journal of the Association for Laboratory Automation ◽

10.1016/s1535-5535-04-00049-8 ◽

2000 ◽

Vol 5 (1) ◽

pp. 45-48 ◽

Cited By ~ 2

Author(s):

Maura C. Kibbey ◽

David MacAllan ◽

James W. Karaszkiewicz

Keyword(s):

Drug Discovery ◽

High Precision ◽

High Throughput ◽

High Performance ◽

Dynamic Range ◽

Wide Dynamic Range ◽

Receptor Ligand ◽

Enzymatic Assays ◽

Detection Systems ◽

Protein Assays

IGEN's ORIGEN® technology, which is based on electrochemiluminescence, has been adopted by a number of research and bioanalytical laboratories who have recognized its exquisite sensitivity, high precision, wide dynamic range, and flexibility in formatting a wide variety of applications. IGEN's M-SERIES™ marks the introduction of the second generation of detection systems employing the ORIGEN technology specifically repackaged to address the needs of the high throughput laboratories involved in drug discovery. Assays are formatted without wash steps. Users realize the high performance of a heterogeneous technology with the convenience of a homogeneous format. The M-SERIES platform can address enzymatic assays (kinases, proteases, helicases, etc.), receptor-ligand or protein-protein assays, immunoassays, quantitation of nucleic acids, as well as other applications. Recent assay formats will be explored in detail.

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Multi-chamber microfluidic platform for high-precision skin permeation testing

Lab on a Chip ◽

10.1039/c6lc01574c ◽

2017 ◽

Vol 17 (9) ◽

pp. 1625-1634 ◽

Cited By ~ 15

Author(s):

M. Alberti ◽

Y. Dancik ◽

G. Sriram ◽

B. Wu ◽

Y. L. Teo ◽

...

Keyword(s):

High Precision ◽

High Throughput ◽

Skin Permeation ◽

Toxicity Testing ◽

Skin Penetration ◽

Microfluidic Platform

We validated a novel microfluidic permeation array for high-precision and high-throughput skin penetration and toxicity testing or screening of chemicals.

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Non-invasive approaches for phenotyping of enhanced performance traits in bean

Functional Plant Biology ◽

10.1071/fp11164 ◽

2011 ◽

Vol 38 (12) ◽

pp. 968 ◽

Cited By ~ 86

Author(s):

Uwe Rascher ◽

Stephan Blossfeld ◽

Fabio Fiorani ◽

Siegfried Jahnke ◽

Marcus Jansen ◽

...

Keyword(s):

High Precision ◽

High Throughput ◽

Resource Use ◽

High Throughput Screening ◽

Resource Competition ◽

Plant Phenotyping ◽

Plant Structure ◽

Resource Use Efficiency ◽

Use Efficiency ◽

Structural Traits

Plant phenotyping is an emerging discipline in plant biology. Quantitative measurements of functional and structural traits help to better understand gene–environment interactions and support breeding for improved resource use efficiency of important crops such as bean (Phaseolus vulgaris L.). Here we provide an overview of state-of-the-art phenotyping approaches addressing three aspects of resource use efficiency in plants: belowground roots, aboveground shoots and transport/allocation processes. We demonstrate the capacity of high-precision methods to measure plant function or structural traits non-invasively, stating examples wherever possible. Ideally, high-precision methods are complemented by fast and high-throughput technologies. High-throughput phenotyping can be applied in the laboratory using automated data acquisition, as well as in the field, where imaging spectroscopy opens a new path to understand plant function non-invasively. For example, we demonstrate how magnetic resonance imaging (MRI) can resolve root structure and separate root systems under resource competition, how automated fluorescence imaging (PAM fluorometry) in combination with automated shape detection allows for high-throughput screening of photosynthetic traits and how imaging spectrometers can be used to quantify pigment concentration, sun-induced fluorescence and potentially photosynthetic quantum yield. We propose that these phenotyping techniques, combined with mechanistic knowledge on plant structure–function relationships, will open new research directions in whole-plant ecophysiology and may assist breeding for varieties with enhanced resource use efficiency varieties.

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