scholarly journals Transfection efficiency and toxicity following delivery of naked plasmid DNA and cationic lipid–DNA complexes to ovine lung segments

2003 ◽  
Vol 8 (4) ◽  
pp. 646-653 ◽  
Author(s):  
Michael Emerson ◽  
Louise Renwick ◽  
Stephen Tate ◽  
Susan Rhind ◽  
Elspeth Milne ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Cationic Lipid ◽  
Dna Complexes ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

In vivo expression and antitumor activity of p53 gene transfer with naked plasmid DNA in an ovarian cancer xenograft model in nude mice

2006 ◽  
Vol 32 (5) ◽  
pp. 449-453 ◽  
Author(s):  
Pierre Collinet ◽  
Rodolphe Vereecque ◽  
Frédéric Sabban ◽  
Denis Vinatier ◽  
Eric Leblanc ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gene Transfer ◽  
Antitumor Activity ◽  
Nude Mice ◽  
Plasmid Dna ◽  
Xenograft Model ◽  
P53 Gene ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Rubbing Gastric Serosal Surface Enhances Naked Plasmid DNA Transfer in Rats and Mice

2011 ◽  
Vol 34 (9) ◽  
pp. 1514-1517 ◽  
Author(s):  
Toyoharu Mine ◽  
Hiroki Ishii ◽  
Sayuri Nakajima ◽  
Naoki Yoshikawa ◽  
Hirotaka Miyamoto ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Dna Transfer ◽  
Serosal Surface ◽  
Naked Plasmid ◽  
Rats And Mice ◽  
Naked Plasmid Dna

scholarly journals Naked plasmid DNA transfer to the porcine liver using rapid injection with large volume

Gene Therapy ◽  
2006 ◽  
Vol 13 (24) ◽  
pp. 1696-1702 ◽  
Author(s):  
H Yoshino ◽  
K Hashizume ◽  
E Kobayashi
Keyword(s):  
Large Volume ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Porcine Liver ◽  
Rapid Injection ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

scholarly journals Pretreatment with Epidermal Growth Factor Enhances Naked Plasmid DNA Transfer onto Gastric Serosal Surface in Mice

2012 ◽  
Vol 35 (6) ◽  
pp. 903-908 ◽  
Author(s):  
Hirotaka Miyamoto ◽  
Sumie Baba ◽  
Sayuri Nakajima ◽  
Toyoharu Mine ◽  
Naoki Yoshikawa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Plasmid Dna ◽  
Dna Transfer ◽  
Serosal Surface ◽  
Epidermal Growth ◽  
Naked Plasmid ◽  
Naked Plasmid Dna

Cholic Acid-Modified Polyethylenimine for Gene Delivery into Human Carcinoma Cells

2014 ◽  
Vol 1060 ◽  
pp. 3-6 ◽  
Author(s):  
Wanlop Weecharangsan ◽  
Orapan Paecharoenchai ◽  
Nattisa Niyomtham ◽  
Praneet Opanasopit ◽  
Boon-ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Zeta Potential ◽  
Cholic Acid ◽  
Plasmid Dna ◽  
Gel Electrophoresis ◽  
Transfection Efficiency ◽  
Carcinoma Cells ◽  
Molar Ratio ◽  
Dna Complexes ◽  
Human Carcinoma

Polyethylenimine (PEI) was modified by cholic acid at a molar ratio of 1:1. Cholic acid (CA)-modified PEI (PEI-CA) were evaluated for formation of DNA complexes. PEI-CA/pEGFP plasmid DNA complexes were characterized for their size and zeta potential. Gel electrophoresis showed total retardation for PEI-CA/pEGFP complexes formed at weight ratios above 0.25. The particle size and zeta potential of the complexes at a polymer-to-DNA ratio of 0.5 were 295.3 nm and 30.5 mV, respectively. The transfection efficiency of PEI-CA/pEGFP complexes was comparable to unmodified PEI. Cytotoxicity result showed that PEI-CA had lower cytoxicity than PEI. This study suggests that PEI-CA has potential utility as a gene delivery carrier.

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