474. Baculovirus Vector for Mesenchymal Stem Cells Engineering and Calvarial Bone Repair

Large bone defects are a medical concern as these are often unable to heal spontaneously, based on the host bone repair mechanisms. In their treatment, bone tissue engineering techniques represent a promising approach by providing a guide for osseous regeneration. As bioactive glasses proved to have osteoconductive and osteoinductive properties, the aim of our study was to evaluate by histologic examination, the differences in the healing of critical-sized calvarial bone defects filled with bioactive glass combined with adipose-derived mesenchymal stem cells, compared to negative controls. We used 16 male Wistar rats subjected to a specific protocol based on which 2 calvarial bone defects were created in each animal, one was filled with Bon Alive S53P4 bioactive glass and adipose-derived stem cells and the other one was considered control. At intervals of one week during the following month, the animals were euthanized and the specimens from bone defects were histologically examined and compared. The results showed that this biomaterial was biocompatible and the first signs of osseous healing appeared in the third week. Bone Alive S53P4 bioactive glass could be an excellent bone substitute, reducing the need of bone grafts.

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Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells

Biomaterials ◽

10.1016/j.biomaterials.2007.10.006 ◽

2008 ◽

Vol 29 (4) ◽

pp. 427-437 ◽

Cited By ~ 64

Author(s):

Irene R. Dégano ◽

Marta Vilalta ◽

Juli R. Bagó ◽

Annette M. Matthies ◽

Jeffrey A. Hubbell ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Bone Repair ◽

Bioluminescence Imaging ◽

Calvarial Bone

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Xenotransplantation of Human Mesenchymal Stem Cells into Immunocompetent Rats for Calvarial Bone Repair

Tissue Engineering Part A ◽

10.1089/ten.tea.2009.0401 ◽

2010 ◽

Vol 16 (2) ◽

pp. 479-488 ◽

Cited By ~ 34

Author(s):

Ching-Kuang Chuang ◽

Kun-Ju Lin ◽

Chin-Yu Lin ◽

Yu-Han Chang ◽

Tzu-Chen Yen ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Repair ◽

Human Mesenchymal Stem Cells ◽

Calvarial Bone

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Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652012000300026 ◽

2012 ◽

Vol 84 (3) ◽

pp. 841-851 ◽

Cited By ~ 9

Author(s):

Betânia S. Monteiro ◽

Napoleão M. Argôlo-Neto ◽

Nance B. Nardi ◽

Pedro C. Chagastelles ◽

Pablo H. Carvalho ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Bone Repair ◽

Bone Matrix ◽

Cell Types ◽

Repair Process ◽

Treated Group ◽

Control Group ◽

Calvarial Bone

Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp+ were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that observed in the control group. The results demonstrated that transplantation of bone marrow-derived MSC of C57BL/6 gfp+ mice to bone critical defects produced in mice calvarial contributes positively to the bone repair process. MSC presets ability to influence the correct functioning of osteoblasts, increases the amount of mobilized cells for the repairing process, speeds up growth, and increases deposition of bone matrix.

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Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502012000300001 ◽

2012 ◽

Vol 27 (3) ◽

pp. 201-209 ◽

Cited By ~ 12

Author(s):

Betânia Souza Monteiro ◽

Ricardo Junqueira Del Carlo ◽

Napoleão Martins Argôlo-Neto ◽

Nance Beyer Nardi ◽

Pablo Herthel Carvalho ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Repair ◽

Platelet Rich Plasma ◽

Treated Group ◽

Control Group ◽

Calvarial Bone ◽

Cranial Defect ◽

Calvarial Defects ◽

Receive Treatment

PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.

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In vivo Evaluation of a Collagen Scaffold Preconditioned with Adipose-derived Mesenchymal Stem Cells Used for Bone Regeneration A histological study

Materiale Plastice ◽

10.37358/mp.18.4.5102 ◽

2018 ◽

Vol 55 (4) ◽

pp. 691-695

Author(s):

Tudor Sorin Pop ◽

Anca Maria Pop ◽

Alina Dia Trambitas Miron ◽

Klara Brinzaniuc ◽

Simona Gurzu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Regeneration ◽

Histological Study ◽

Collagen Scaffold ◽

Bone Defects ◽

Collagen Scaffolds ◽

In Vivo Evaluation ◽

Calvarial Bone ◽

Bone Apposition

The use of collagen scaffolds and stem cells for obtaining a tissue-engineering complex has been an important concept in promoting repair and regeneration of the bone tissue. Such units represent important steps in the development of an ideal scaffold-cell complex that would sustain new bone apposition. The aim of our study was to perform a histologic evaluation of the healing of critical-sized bone defects, using a biologic collagen scaffold with adipose-derived mesenchymal stem cells, in comparison to negative controls created in the adjacent bone. We used 16 Wistar rats and according to the study design 2 calvarial bone defects were created in each animal, one was filled with collagen seeded with adipose-derived stem cells and the other one was considered negative control. During the following month, at weekly intervals, the animals were euthanized and the specimens from bone defects were histologically evaluated. The results showed that these scaffolds were highly biocompatible as only moderate inflammation no rejection reactions were observed. Furthermore, the first signs of osseous healing appeared after two weeks accompanied by angiogenesis. Collagen scaffolds seeded with adipose-derived mesenchymal stem cells can be considered a promising treatment option in bone regeneration of large defects.

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