Abstract: P684 SHORT-TERM SPACEFLIGHT ENHANCES PROTEIN AND GENE EXPRESSION OF INTERLEUKIN 6 AND INTERCELLULAR CELL ADHESION MOLECULE-1 IN ENDOTHELIAL CELLS

2009 ◽  
Vol 10 (2) ◽  
pp. e870
Author(s):  
S Muid ◽  
G Froemming ◽  
K Yusoff ◽  
M Ali ◽  
H Nawawi
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Interleukin 6 ◽  
Cell Adhesion Molecule ◽  
Short Term ◽  
Cell Adhesion Molecule 1 ◽  
Intercellular Cell Adhesion Molecule

Synbiotic Cheese from Goat Milk Suppresses Indomethacin-induced Intercellular Cell Adhesion Molecule-1 (ICAM-1) Expression in a Rat Model of GastricInjury

2019 ◽  
Vol 14 (1) ◽  
pp. 32-36
Author(s):  
Salmi ◽  
Nurliyani ◽  
Sunarti
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Rat Model ◽  
Cell Adhesion Molecule ◽  
Goat Milk ◽  
Gastric Injury ◽  
Protein Levels ◽  
Cell Adhesion Molecule 1 ◽  
Intercellular Cell Adhesion Molecule

Synbiotic cheese made of goat milk, bacterial starter Lactobacillus rhamnosus, and porang glucomannan has been reported to have anti-inflammatory effects. This study aimed to determine the effect of synbiotic cheese on gene expression and protein levels of intercellular cell adhesion molecule-1 in a rat model of gastric injury. Male Wistar rats were divided into six groups. For 28 days, three groups received an increasing dosage of synbiotic cheese and one group received one dosage of probiotic cheese. For comparison, there was a placebo group receiving nothing and another group receiving indomethacin alone. On day 29, all rats received 20 mg/kg indomethacin intragastrically to induce gastric injury. Twenty-four hours later, rats were euthanized, and gastric tissue was taken for the quantification of intercellular cell adhesion molecule-1 gene and protein expressions. The results showed that pretreatment of synbiotic cheese caused significant suppression of intercellular cell adhesion molecule-1 expression. Synbiotic cheese at a dose of 0.36 g/day significantly suppressed intercellular cell adhesion molecule-1 protein expression (P < 0.05), whereas synbiotic cheese at a dose of 0.72 g/day significantly suppressed both gene expression and protein levels of intercellular cell adhesion molecule-1 (P < 0.05) compared to the indomethacin alone group. We conclude that synbiotic cheese may protect from gastric injury through modulation of intercellular cell adhesion molecule-1.

scholarly journals Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells

2019 ◽  
Vol 20 (21) ◽  
pp. 5383 ◽  
Author(s):  
Li Zhang ◽  
Feifei Wang ◽  
Qing Zhang ◽  
Qiuming Liang ◽  
Shumei Wang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Umbilical Vein ◽  
Caspase 9 ◽  
Protein Levels ◽  
Tnf Α ◽  
Umbilical Vein Endothelial Cells ◽  
Cell Adhesion Molecule 1

Inflammation is a key mediator in the progression of atherosclerosis (AS). Benzoinum, a resin secreted from the bark of Styrax tonkinensis, has been widely used as a form of traditional Chinese medicine in clinical settings to enhance cardiovascular function, but the active components of the resin responsible for those pharmaceutical effects remain unclear. To better clarify these components, a new phenylpropane derivative termed stybenpropol A was isolated from benzoinum and characterized via comprehensive spectra a nalysis. We further assessed how this phenylpropane derivative affected treatment of human umbilical vein endothelial cells (HUVECs) with tumor necrosis factor-α (TNF-α). Our results revealed that stybenpropol A reduced soluble intercellular cell adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion molecule-1 (sVCAM-1), interleukin-8 (IL-8), and interleukin-1β (IL-1β) expression by ELISA, inhibited apoptosis, and accelerated nitric oxide (NO) release in TNF-α-treated HUVECs. We further found that stybenpropol A decreased VCAM-1, ICAM-1, Bax, and caspase-9 protein levels, and increased the protein levels of Bcl-2, IKK-β, and IκB-α. This study identified a new, natural phenylpropane derivative of benzoinum, and is the first to reveal its cytoprotective effects in the context of TNF-α-treated HUVECs via regulation of the NF-κB and caspase-9 signaling pathways.

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