Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma

Settings. Despite the limited diagnostic utility of AgNORs (argyrophilic nucleolar organiser region-associated proteins) for individual breast lesions, AgNOR analysis bears a significant potential for characterizing cell proliferative activity of breast lesions. Methodology. The present study investigated the relationship between mean AgNORs count and immunohistochemical expression of ER, PR, HER2/neu, and p53 in breast carcinoma in serial paraffin sections from 137 breast carcinomas. Twenty control cases of benign breast lesions were included. Results. Mean AgNOR counts correlated significantly inversely with hormone estrogen receptors (ER), Progesterone receptors (PR), and p53 immunohistochemical expression, denoting values of 0.05, 0.01, and 0.001, respectively. No significant correlation was found between mean AgNOR counts and HER2/neu, . Mean AgNOR count was significantly higher in grade II tumor cells. We conclude that mean AgNOR counts correlate with ER, PR, and P53 tumor markers in breast carcinomas. Conclusion. We recommend the use of mean AgNOR count for accurate reporting of breast carcinomas, as well as prediction of ER, PR, and P53 in routine paraffin sections.

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Oestrogen and progesterone receptors in screen-detected breast carcinoma: an immunohistological study using paraffin sections

Histopathology ◽

10.1111/j.1365-2559.1992.tb00442.x ◽

1992 ◽

Vol 21 (6) ◽

pp. 543-547 ◽

Cited By ~ 18

Author(s):

S. SOOMRO ◽

S. SHOUSHA ◽

H.D. SINNETT

Keyword(s):

Breast Carcinoma ◽

Progesterone Receptors ◽

Paraffin Sections ◽

Immunohistological Study

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Duodenal metastasis of breast invasive carcinoma of no special type: a rare presentation

Acta Gastro Enterologica Belgica ◽

10.51821/84.2.379 ◽

2021 ◽

Vol 84 (2) ◽

pp. 379-380

Author(s):

M Mascarenhas Saraiva ◽

T Ribeiro ◽

A.R. Coelho ◽

R Silva ◽

A.C. Real ◽

...

Keyword(s):

Breast Carcinoma ◽

Epigastric Pain ◽

Growth Factor Receptor ◽

Progesterone Receptors ◽

Ca 125 ◽

Her2 Positive ◽

Rare Presentation ◽

History Of ◽

Lobular Breast Carcinoma ◽

Epidermal Growth

The authors read with attention the case report by Vande Berg and coworkers, published on a previous issue of this journal, describing a rare diagnosis of rectal metastases of an incognito invasive lobular breast carcinoma (ILC), for which immunohistochemistry was decisive (1). Likewise, we share a similarly rare case of gastrointestinal metastases of breast carcinoma (BC), in which immunohistochemistry played a pivotal role.A 55-year-old woman was diagnosed in June 2015 with an Invasive Breast Carcinoma of no-special-type (IBCNST) grade III, human epidermal growth factor receptor 2 (HER2) positive, estrogen and progesterone receptors (ER/PR) negative. Biopsy revealed vascular permeation. Neoadjuvant chemotherapy and trastuzumab preceded surgery, performed in December 2015, followed by radiotherapy and trastuzumab. The patient remained free of disease until 2017, when a right cerebellar lesion was diagnosed as BC metastasis and was treated with stereotactic radiosurgery. In January 2020, the patient presented with a 1-month history of post-prandial vomiting, epigastric pain, anorexia, fatigue, and a 10 kg weight loss. Lab tests were unremarkable, except for small increase in CA-125 and CA-15.3.

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Estrogen and Progesterone Receptors in Breast Carcinoma and in Nonmalignant Breast Tissue

Tumori Journal ◽

10.1177/030089168507100511 ◽

1985 ◽

Vol 71 (5) ◽

pp. 477-481 ◽

Cited By ~ 5

Author(s):

Massimo Gion ◽

Riccardo Mione ◽

Ruggero Dittadi ◽

Luciano Griggio ◽

Gabriele Munegato ◽

...

Keyword(s):

Breast Carcinoma ◽

Estrogen Receptors ◽

Breast Tissue ◽

Progesterone Receptors ◽

Estrogen And Progesterone Receptors ◽

Receptor Level ◽

Receptor Status ◽

Benign Tissue ◽

Receptor Concentration ◽

The Relationship

Since 1983 we have studied the relationship, in the same patient, between receptor status in breast carcinoma and in nonmalignant breast tissue. Fifty patients have been evaluated to date. The total unoccupied cytosol estrogen and progesterone receptors were determined by a dextran-coated charcoal method. In nonmalignant breast tissue we found a measurable receptor concentration above the sensitivity of the method in 62 % of cases for estrogen receptors and in 44 % of cases for progesterone receptors. No relationships were found between the receptor level of each tumor and that of the corresponding benign tissue. The data suggest that the levels of the receptors in the tumor and in the nonmalignant tissue are totally independent.

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Concordance of histology type between synchronous and metachronous contralateral breast carcinoma: Is there susceptibility for breast cancer of particular histology?

Journal of Clinical Oncology ◽

10.1200/jco.2012.30.15_suppl.e21030 ◽

2012 ◽

Vol 30 (15_suppl) ◽

pp. e21030-e21030

Author(s):

Zorica Tomasevic ◽

Dusica Gavrilovic ◽

Zoran Tomasevic ◽

Daniela Kolarevic

Keyword(s):

Breast Cancer ◽

Breast Carcinoma ◽

Statistical Difference ◽

Lobular Carcinoma ◽

Progesterone Receptors ◽

Distant Metastases ◽

Tumor Grade ◽

Contralateral Breast ◽

Molecular Characteristics ◽

Significant Difference

e21030 Background: Contra-lateral breast cancer (CBC) is considered to be the most frequent new malignancy after primary breast cancer (PBC) However, there are few reports whether the histology and other major molecular characteristics differs between PBC and CBC. The aim of this paper is toevaluate and compare PBC/CBC histology according to the time to CBC development. Also, age at CBC and major molecular characteristics (tumor grade, estrogen/progesterone receptors, HER2) have been analyzed. Methods: A cohort of 113 patients with CBC, without distant metastases, has been prospectively registered during 28 months. Patients are divided in 2 groups according to the time of CBC diagnose: 1. Synchronous, if the CBC (S-CBC) was diagnosed either simultaneously or within 6 months after PBC; 2. Metachronous (M-CBC) if CBC was diagnosed > 6 months after PBC. Results: Results are presented in Table 1. Median age was calculated at the time of CBC diagnose. Patient with S-CBC are median 7 years older than patients with M- CBC that is statistically significant difference (p-0.007) S-CBC is more likely to be of the same histological type (76%) than M-CBC (56%) (p 0.006) In the whole analyzed group, and each subgroup separately, lobular carcinoma is registered in higher percentage (41%) than expected (~20%) For all other analyzed BC characteristics, statistical difference was not confirmed, however ~ 25% of specimens were not available for testing. Conclusions: Although it is still unclear what could be the clinical significance of these findings, genetic susceptibility to particular histology type of BC might be reasonably speculated. [Table: see text]

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