scholarly journals Ixodes kangdingensis (Acari: Ixodidae), a new species from the Siberian weasel, Mustela sibirica (Carnivora: Mustelidae) in China

2017 ◽  
Vol 3 ◽  
Author(s):  
TIANYU GUO ◽  
YI SUN ◽  
GUANG XU ◽  
LANCE A. DURDEN
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Divergence ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Ixodes Arboricola ◽  
A New Species

SUMMARY The female and nymph of Ixodes (Pholeoixodes) kangdingensis n. sp. are described based on both morphology and analysis of 16S rRNA gene sequences. Specimens of this new tick species were collected from a Siberian weasel (Mustela sibirica) in Kangding County, Sichuan Province, China. The morphological features of the female and nymph are unique to distinguish I. kangdingensis n. sp. from other members of the subgenus Pholeoixodes, including the presence of distinctly shaped cornua, anterior and posterior processes on palpal article I and a large angular projection on each side of the hypostome. Partial sequence of 16S rRNA gene grouped this species with Ixodes arboricola and Ixodes lividus with sequence divergence of new species from I. arboricola 4·16% and from I. lividus 8·49%. Data on the phylogenetic position, hosts, geographic distribution and key to females of closely related species are also provided.

scholarly journals Phylogenetic position reevaluation of Kyrtuthrix and description of a new species K. huatulcensis from Mexico´s Pacific coast

Phytotaxa ◽  
2016 ◽  
Vol 278 (1) ◽  
pp. 1 ◽  
Author(s):  
HILDA P. LEÓN-TEJERA ◽  
LAURA GONZÁLEZ-RESENDIZ ◽  
JEFFREY R. JOHANSEN ◽  
CLAUDIA SEGAL-KISCHINEVSKY ◽  
VIVIANA ESCOBAR ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Data ◽  
Geographical Information ◽  
Morphological Data ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Structure Comparison ◽  
A New Species

Benthic marine heterocytous cyanoprokaryotes of Mexico´s tropical coast are being recognized as an important and conspicuous component of the supralittoral and intertidal zones usually described as an extreme and low diversity biotope. Although Kyrtuthrix has been reported from different coasts worldwide, its complex morphology has led to differing taxonomic interpretations and positioning. Ten marine supra and intertidal populations of Kyrtuthrix were analyzed using a detailed morphological approach, complemented with ecological and geographical information as well as DNA sequence data of the 16S rRNA gene and associated 16S–23S ITS. Kyrtuthrix huatulcensis is described as a new species, different from K. dalmatica Ercegovic and K. maculans (Gomont) Umezaki based primarily on morphological data. Our material has smaller dimensions in thalli, filaments, trichomes and cells, and possesses differences in qualitative characters as well. Our material is epilithic as in K. maculans but not endolithic as in K. dalmatica. The analysis of molecular data, mainly 16S rRNA gene sequence, show that this genus is within the Rivulariaceae clade, and outside of the Scytonemataceae clade. ITS secondary structure comparison with some of the closer representatives of Rivulariaceae shows considerable differences with these related genera. Based on the evidence gathered in this study, inclusion of Kyrtuthrix within the Rivulariaceae, rather than the Scytonemataceae, is fully supported.

scholarly journals Cnemaspis flaviventralis, a new species of gecko (Squamata: Gekkonidae) from the Western Ghats of Maharashtra, India

2016 ◽  
Vol 8 (14) ◽  
pp. 9619
Author(s):  
Amit Sayyed ◽  
Robert Alexander Pyron ◽  
Neelesh Dahanukar
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Western Ghats ◽  
Sympatric Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Mitochondrial 16S Rrna Gene ◽  
The Western Ghats ◽  
A New Species

Cnemaspis flaviventralis, a new species of day gecko, is described from the forests of Amboli, Sindhudurg District, Maharashtra State, northern Western Ghats, India.  The new species was previously confused with the sympatric species Cnemaspis girii, C. indraneildasii, C. kolhapurensis and C. goaensis.  It is distinguished from C. giri by having spine-like tubercles on flanks, granular dorsal scales intermixed with large, depressed, slightly keeled scales (vs. lack of spine-like tubercles on flanks, granular dorsal scales, intermixed with large smooth scales); from C. indraneildasii by having dorsal scales heterogeneous (vs. homogenous), lacking a series of enlarged median sub-caudal scales, and 28–29 (vs. 20) ventral scales across mid-body; from C. kolhapurensis by having heterogeneous (vs. homogenous) dorsal scalation, lacking spine-like tubercles on flanks and lacking pre-cloacal pores (vs. 24–28 pre-cloacal-femoral pores); and from C. goaensis by lacking pre-cloacal pores and lacking a series of enlarged median sub-caudal scales.  We further provide partial mitochondrial 16S rRNA gene sequences for the new species and for the sympatric species C. girii, C. kolhapurensis and C. goaensis, and show that the new species is genetically distinct.  

scholarly journals Amplification of 16S rRNA gene sequences to differentiate two highly related bradyrhizobia species

2007 ◽  
Vol 42 (9) ◽  
pp. 1361-1364 ◽  
Author(s):  
Adriana Giongo ◽  
Adriana Ambrosini ◽  
João Ruy Jardim Freire ◽  
Maria Helena Bodanese Zanettini ◽  
Luciane Maria Pereira Passaglia
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bradyrhizobium Japonicum ◽  
Diagnostic Method ◽  
Molecular Diagnostic ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Reference Strains ◽  
Commercial Inoculants

A 16S rRNA gene PCR-based assay was developed aiming at a fast molecular diagnostic method to differentiate the two phylogenetically closely related species Bradyrhizobium japonicum and B. elkanii, isolated from soybean nodules, in order to identify those more competitive and comprising greater nitrogen fixation ability for use in the formulation of commercial inoculants. The assay used was able to discriminate ten reference strains belonging to these two Bradyrhizobium species, as well as to efficiently identify 37 strains isolated from fields cultivated with soybean.

scholarly journals Asgard archaea are diverse, ubiquitous, and transcriptionally active microbes

2018 ◽  
Author(s):  
Mingwei Cai ◽  
Yang Liu ◽  
Zhichao Zhou ◽  
Yuchun Yang ◽  
Jie Pan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Genomic Analysis ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Group D ◽  
Origin Of Eukaryotes

AbstractAsgard is a newly proposed archaeal superphylum. Phylogenetic position of Asgard archaea and its relationships to the origin of eukaryotes is attracting increasingly research interest. However, in-depth knowledge of their diversity, distribution, and activity of Asgard archaea remains limited. Here, we used phylogenetic analysis to cluster the publicly available Asgard archaeal 16S rRNA gene sequences into 13 subgroups, including five previously unknown subgroups. These lineages were widely distributed in anaerobic environments, with the majority of 16S rRNA gene sequences (92%) originating from sediment habitats. Co-occurrence analysis revealed potential relationships between Asgard, Bathyarchaeota, and Marine Benthic Group D archaea. Genomic analysis suggested that Asgard archaea are potentially mixotrophic microbes with divergent metabolic capabilities. Importantly, metatranscriptomics confirmed the versatile lifestyles of Lokiarchaeota and Thorarchaeota, which can fix CO2using the tetrahydromethanopterin Wood-Ljungdahl pathway, perform acetogenesis, and degrade organic matters. Overall, this study broadens the understandings of Asgard archaea ecology, and also provides the first evidence to support a transcriptionally active mixotrophic lifestyle of Asgard archaea, shedding light on the potential roles of these microorganisms in the global biogeochemical cycling.

A new species of Megophrys Kuhl & Van Hasselt (Amphibia: Megophryidae) from Borneo allied to M. nasuta (Schlegel, 1858)

Zootaxa ◽  
2019 ◽  
Vol 4679 (1) ◽  
pp. 1-24 ◽  
Author(s):  
MISBAHUL MUNIR ◽  
AMIR HAMIDY ◽  
MASAFUMI MATSUI ◽  
DJOKO T. ISKANDAR ◽  
IRVAN SIDIK ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Genetic Distance ◽  
Advertisement Call ◽  
Mountain Forest ◽  
Rrna Gene ◽  
Mitochondrial 16S Rrna Gene ◽  
A New Species

Within Sundaland, the species diversity of the genus Megophrys is richest in Borneo, with some species restricted to the northern highlands of the island. On the basis of molecular, morphological and acoustic evidence, we describe a new species of large-sized Megophrys, once confused with M. nasuta. The new species Megophrys kalimantanensis sp. nov. can be differentiated from all its congeners by large genetic distance more than 6.11% in the mitochondrial 16S rRNA gene, much shorter rostral and orbital appendages, and unique advertisement call. This new species has been recorded from medium to high altitudes of mountain forest in southern and northernmost Borneo. 

scholarly journals Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2072-2078 ◽  
Author(s):  
David Durán ◽  
Luis Rey ◽  
Juan Mayo ◽  
Doris Zúñiga-Dávila ◽  
Juan Imperial ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Closely Related Species ◽  
Content Type ◽  
Link Type

A group of strains isolated from root nodules of Phaseolus lunatus (Lima bean) in Peru were characterized by genotypic, genomic and phenotypic methods. All strains possessed identical 16S rRNA gene sequences that were 99.9 % identical to that of Bradyrhizobium lablabi CCBAU 23086T. Despite having identical 16S rRNA gene sequences, the Phaseolus lunatus strains could be divided into two clades by sequence analysis of recA, atpD, glnII, dnaK and gyrB genes. The genome sequence of a representative of each clade was obtained and compared to the genomes of closely related species of the genus Bradyrhizobium . Average nucleotide identity values below the species circumscription threshold were obtained when comparing the two clades to each other (88.6 %) and with all type strains of the genus Bradyrhizobium (≤92.9 %). Phenotypes distinguishing both clades from all described and closely related species of the genus Bradyrhizobium were found. On the basis of the results obtained, two novel species, Bradyrhizobium paxllaeri sp. nov. (type strain LMTR 21T = DSM 18454T = HAMBI 2911T) and Bradyrhizobium icense sp. nov. (type strain LMTR 13T = HAMBI 3584T = CECT 8509T = CNPSo 2583T), are proposed to accommodate the uncovered clades of Phaseolus lunatus bradyrhizobia. These species share highly related but distinct nifH and nodC symbiosis genes.

A new species of the processid shrimp genus Nikoides Paul’son, 1875 (Decapoda: Caridea) from Japan

Zootaxa ◽  
2021 ◽  
Vol 5057 (1) ◽  
pp. 114-126
Author(s):  
TOMOYUKI KOMAI ◽  
ISAO HIRABAYASHI
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Genetic Divergence ◽  
Identification Key ◽  
Rrna Gene ◽  
Characteristic Shape ◽  
Mitochondrial 16S Rrna Gene ◽  
A New Species

A new species of the processid shrimp genus Nikoides Paul’son, 1875, N. subdistalis, is described and illustrated on the basis of five specimens collected from Kushimoto, Wakayama Prefecture, Japan, at depths of 7–12 m. The new species appears most similar to N. maldivensis Borradaile, 1915 among the 10 known congeners, but is notable in the unique dentition of the rostrum and the characteristic shape of the antennular stylocerite. A partial segment of the mitochondrial 16S rRNA gene was sequenced from one of the paratypes of the new species, and genetic divergence among four congeneric taxa, of which three was downloaded from the GenBank database, is shown. An identification key to 11 species of Nikoides, including the new species, is given.  

scholarly journals Massilia dura sp. nov., Massilia albidiflava sp. nov., Massilia plicata sp. nov. and Massilia lutea sp. nov., isolated from soils in China

2006 ◽  
Vol 56 (2) ◽  
pp. 459-463 ◽  
Author(s):  
Yu-Qin Zhang ◽  
Wen-Jun Li ◽  
Ke-Yun Zhang ◽  
Xin-Peng Tian ◽  
Yi Jiang ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Hybridization ◽  
Type Strain ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Gene Sequence Analysis

Four Gram-negative, motile, rod-shaped bacterial strains were isolated from soil samples collected from south-east China. A taxonomic study including phylogenetic analysis based on 16S rRNA gene sequences and phenotypic characteristics was performed. DNA G+C contents of the four strains were 63–66 mol%. Their predominant ubiquinone was Q-8. The fatty acid profiles contained C16 : 1 ω7c (36·9–54·7 %) and C16 : 0 (22·8–25·5 %) as the major components. Based on their phenotypic characteristics, phylogenetic position as determined by 16S rRNA gene sequence analysis and DNA–DNA hybridization results, the four isolates are considered to represent four novel species of the genus Massilia, for which the names Massilia dura sp. nov. (type strain 16T=CCTCC AB 204070T=KCTC 12342T), Massilia albidiflava sp. nov. (type strain 45T=CCTCC AB 204071T=KCTC 12343T), Massilia plicata sp. nov. (type strain 76T=CCTCC AB 204072T=KCTC 12344T) and Massilia lutea sp. nov. (type strain 101T=CCTCC AB 204073T=KCTC 12345T) are proposed.

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