Identification of Tetranychus neocaledonicus (Acari: Tetranychidae) resistant genotypes in Phaseolus lunatus

The spider mite Tetranychus neocaledonicus André (Acari: Tetranychidae) is a potential threat to the cultivation of lima bean (Phaseolus lunatus L.). In many countries, lima beans are grown by small-holder farmers with limited access to inputs and technologies. In such systems, the implementation of control practices, such as chemical treatments that require costly equipment and supplies, becomes unviable. Therefore, the development and use of resistant plant genotypes could provide a promising alternative for small producers. Accordingly, the present study aimed to identify and evaluate T. neocaledonicus-resistant genotypes of lima bean. A total of 30 lima bean genotypes were evaluated using the instantaneous increase rate (ri) of T. neocaledonicus reared on individual host genotypes, and T. neocaledonicus fertility life tables were generating by rearing mites on host genotypes with the two highest ri values (IFCE-085 and IFCE-091, considered highly susceptible) and two lowest ri values (IFCE-084 and IFCE-090, considered resistant). Only the IFCE-090 genotype adversely affected immature T. neocaledonicus individuals, by reducing their survival rate, net reproductive rate (Ro), finite rate of increase (λ), and intrinsic rate of increase (rm) and by increasing their mean generation time (T) and time to reach adulthood. The results demonstrate the variation in the susceptibility of lima bean genotypes to T. neocaledonicus and identify the IFCE-090 genotype as a potential source of mite resistance.

The administration’s effect of domestic soybean, lablab bean and lima bean content of genistein to improve the productivity of Bali cattle

Bali cattle plays a significant role as producers of food products for humans. Legumes are used in high-quality animal feed to increase productivity. This study analyzed the use ofdomestic Soybean (Glycine max), Lablab bean (Lablab purpureus), and Lima bean (Phaseolus lunatus) as the genistein in Bali cattle feed to shorten postpartum estrus. Different drying methods were applied by putting the ingredients in the oven at 55 ºC and the freeze dryer at -40 ºC.Further, genistein was detected using the Thin Layer Chromatography Method at UV 254 and 366 to determine the Rf value. The parameters measured include genistein detection and genistein identification as well as genistein content in legume straw. The data were analyzed descriptiveusing ANOVA and t-test. The result showed that only two samples adhered to the gel silica plate, including domestic soybean straw using the oven and freeze dryer (DSOD and DSFD), with 0.48 in DSOD and 0.51 in DSFD of Rf value, that is close to the genistein standard with the Rf value of 0.50 and purple colour as the colour of genistein standard. Moreover, the statistical analyses indicated significant differences in the genistein content of legumestraw between DSOD and DSFD (P<0.05), where DSOD=0.662g/100g and DSFD=1.770g/100g. The domestic soybean straw is regarded as a potential source ofBali cattle feed to improve productivity.

Characterization of nutritional and functional properties of Lima bean flour (Phaseolus Lunatus L.)

Lima beans (Phaseolus Lunatus L.) are underutilized crops with an excellent profile. Processing to flour to enhance the starch and protein content, reducing anti-nutritional components and the same time diversifies their use as ingredients by altering their functional properties. The study aims to characterize nutritional, amino acids, anti-nutritional, pasting and functional properties of Lima bean flour from Indonesia’s cultivated plant. The results showed that the Lima bean flour has a high carbohydrate concentration, moderate protein concentration, and low fat concentration. This flour has a balanced amino acid profile, rich in essential amino acids, highlighting them as a source of good quality protein for the food formulation of protein-enriched. The Lima bean flour contained 10.36 mg/g phytic acid, lower in phenolic content (0.63 mg/g) and concentration of HCN (8.83 mg/kg). The functional properties of the Lima bean flour swelling power, solubility, water absorption capacities, and oil absorption capacities were 6.88 g/g, 18.68%, 1.93 g/g, and 1.56 g/g respectively. Pasting properties of Lima bean flour exhibited peak, breakdown, final, and setback viscosity in 1172 cP, 83 cP, 2377 cP, 1288 cP respectively, and temperature pasting was high in 870C. The study may provide useful information to consumers and food manufacturers that Lima bean flour is significant potential used to enhance the nutritional value of cereal-based foods.

Comparison of cyanide content in arbila beans (Phaseolus lunatus l) of East Nusa Tenggara using picrate and acid hydrolysis methods

Arbila beans contains cyanogenic compounds in the form of linamarin (cyanoglucosides), acetone cyanohydrin, and free cyanide, all together constitute total cyanide content. The objective of this study was to compare the cyanide content in arbila beans analyzed by picrate and acid hydrolysis methods. Picrate method measures total cyanide only. Cyanide content was identified by using a picrate paper, which turned into yellow. The absorbance was measured by a spectrophotometer at 510 nm. Acid hydrolysis method measures cyanide in arbila beans in the form of linamarin, acetone cyanohydrin, and free cyanide. Linamarin wa hydrolyzed in H3PO4 solution. Estimated cyanide levels was measured by using a colorimetric procedure. Data was analyzed using Independent sample t-test (SPSS v.16). The results showed that, there was no difference in the total level of cyanide in both methods. Total cyanide measured by picrate and acid hydrolysis method was 2705.17 ppm and 2693.29 ppm, respectively. In addition, the three forms of cyanide content were as follows: linamarin 926.22 ppm, cyanohydrin 556.01 ppm, and free cyanide 1211.06 ppm. Based on the results, both methods can be used for total cyanide analysis. To determine the form of cyanide other than total cyanide, it is recommended to use the acid hydrolysis method.

GENETIC DIVERSITY OF LIMA BEAN (PHASEOLUS LUNATUS L.) FROM TIMOR ISLAND BASED ON MOLECULAR MARKER INTER-SIMPLE SEQUENCE REPEATS (ISSR)

Lima bean (Phaseolus lunatus L.) is one of the important legume vegetables in Indonesia. However, genetic information for these plants is still minimalized, especially on Timor Island. This study aims to analyze the genetic diversity of lima beans from Timor Island based on ISSR molecular markers. A total of 4 accessions of lima beans were analyzed using 3 ISSR primers to produce 15 polymorphic bands with an average of 68.18% polymorphism. The cluster analysis results use the Unweighted Pair Group Methods using Arithmetic averages (UPGMA) method to create a dendrogram that produces two main clusters. There were plain seed and pattern seed group with a similarity coefficient of 0.52. These results indicated that the genetic variation of the lima beans from Timor Island was high. Moreover, the result provides a sutable method for evaluating the genetic diversity of lima beans using the ISSR marker and important information of future lima bean breeding programs.