scholarly journals Phylogenetic position reevaluation of Kyrtuthrix and description of a new species K. huatulcensis from Mexico´s Pacific coast

Phytotaxa ◽  
2016 ◽  
Vol 278 (1) ◽  
pp. 1 ◽  
Author(s):  
HILDA P. LEÓN-TEJERA ◽  
LAURA GONZÁLEZ-RESENDIZ ◽  
JEFFREY R. JOHANSEN ◽  
CLAUDIA SEGAL-KISCHINEVSKY ◽  
VIVIANA ESCOBAR ◽  
...  

Benthic marine heterocytous cyanoprokaryotes of Mexico´s tropical coast are being recognized as an important and conspicuous component of the supralittoral and intertidal zones usually described as an extreme and low diversity biotope. Although Kyrtuthrix has been reported from different coasts worldwide, its complex morphology has led to differing taxonomic interpretations and positioning. Ten marine supra and intertidal populations of Kyrtuthrix were analyzed using a detailed morphological approach, complemented with ecological and geographical information as well as DNA sequence data of the 16S rRNA gene and associated 16S–23S ITS. Kyrtuthrix huatulcensis is described as a new species, different from K. dalmatica Ercegovic and K. maculans (Gomont) Umezaki based primarily on morphological data. Our material has smaller dimensions in thalli, filaments, trichomes and cells, and possesses differences in qualitative characters as well. Our material is epilithic as in K. maculans but not endolithic as in K. dalmatica. The analysis of molecular data, mainly 16S rRNA gene sequence, show that this genus is within the Rivulariaceae clade, and outside of the Scytonemataceae clade. ITS secondary structure comparison with some of the closer representatives of Rivulariaceae shows considerable differences with these related genera. Based on the evidence gathered in this study, inclusion of Kyrtuthrix within the Rivulariaceae, rather than the Scytonemataceae, is fully supported.

2017 ◽  
Vol 3 ◽  
Author(s):  
TIANYU GUO ◽  
YI SUN ◽  
GUANG XU ◽  
LANCE A. DURDEN

SUMMARY The female and nymph of Ixodes (Pholeoixodes) kangdingensis n. sp. are described based on both morphology and analysis of 16S rRNA gene sequences. Specimens of this new tick species were collected from a Siberian weasel (Mustela sibirica) in Kangding County, Sichuan Province, China. The morphological features of the female and nymph are unique to distinguish I. kangdingensis n. sp. from other members of the subgenus Pholeoixodes, including the presence of distinctly shaped cornua, anterior and posterior processes on palpal article I and a large angular projection on each side of the hypostome. Partial sequence of 16S rRNA gene grouped this species with Ixodes arboricola and Ixodes lividus with sequence divergence of new species from I. arboricola 4·16% and from I. lividus 8·49%. Data on the phylogenetic position, hosts, geographic distribution and key to females of closely related species are also provided.


Zootaxa ◽  
2019 ◽  
Vol 4679 (1) ◽  
pp. 1-24 ◽  
Author(s):  
MISBAHUL MUNIR ◽  
AMIR HAMIDY ◽  
MASAFUMI MATSUI ◽  
DJOKO T. ISKANDAR ◽  
IRVAN SIDIK ◽  
...  

Within Sundaland, the species diversity of the genus Megophrys is richest in Borneo, with some species restricted to the northern highlands of the island. On the basis of molecular, morphological and acoustic evidence, we describe a new species of large-sized Megophrys, once confused with M. nasuta. The new species Megophrys kalimantanensis sp. nov. can be differentiated from all its congeners by large genetic distance more than 6.11% in the mitochondrial 16S rRNA gene, much shorter rostral and orbital appendages, and unique advertisement call. This new species has been recorded from medium to high altitudes of mountain forest in southern and northernmost Borneo. 


Zootaxa ◽  
2021 ◽  
Vol 5057 (1) ◽  
pp. 114-126
Author(s):  
TOMOYUKI KOMAI ◽  
ISAO HIRABAYASHI

A new species of the processid shrimp genus Nikoides Paul’son, 1875, N. subdistalis, is described and illustrated on the basis of five specimens collected from Kushimoto, Wakayama Prefecture, Japan, at depths of 7–12 m. The new species appears most similar to N. maldivensis Borradaile, 1915 among the 10 known congeners, but is notable in the unique dentition of the rostrum and the characteristic shape of the antennular stylocerite. A partial segment of the mitochondrial 16S rRNA gene was sequenced from one of the paratypes of the new species, and genetic divergence among four congeneric taxa, of which three was downloaded from the GenBank database, is shown. An identification key to 11 species of Nikoides, including the new species, is given.  


ZooKeys ◽  
2020 ◽  
Vol 923 ◽  
pp. 115-140 ◽  
Author(s):  
Tao Luo ◽  
Ning Xiao ◽  
Kai Gao ◽  
Jiang Zhou

This study describes a new species of the genus Leptobrachella, Leptobrachella suiyangensissp. nov. from the Huoqiuba Nature Reserve, Suiyang County, Guizhou Province, China, based on morphological data and phylogenetic analyses (16S rRNA mtDNA). The new species can be distinguished from other congeners by the molecular divergence and by a combination of morphological characters, including body size, dorsal and ventral patterns, dorsal skin texture, size of the pectoral and femoral glands, degree of webbing and fringing on the toes and fingers, dorsum coloration, and iris coloration in life. Currently, the genus Leptobrachella contains 75 species, 21 of which are found in China, including seven species reported from Guizhou Province. The uncorrected sequence divergence percentage between Leptobrachella suiyangensissp. nov. and all homologous DNA sequences available for the 16S rRNA gene was found to be >4.7%. The new record of the species and its relationships with others in the same genus imply that species distribution, habitat variation, environmental adaptation, and diversity of the genus Leptobrachella in southwest China need to be further investigated.


Crustaceana ◽  
2017 ◽  
Vol 90 (7-10) ◽  
pp. 1267-1277 ◽  
Author(s):  
Yaqin Wang ◽  
Zhibin Gan ◽  
Xinzheng Li

Based on samples collected from the Yellow Sea in October 2014, a new species of pasiphaeid shrimp, Leptochela (Leptochela) tuerkayi sp. nov., is described. The new species is closely related to L. (L.) gracilis Stimpson, 1860 in morphology but differs from the latter and other congeners by the following combination of characters: three longitudinal dorsal carinae in ovigerous females, third maxilliped with the rather short exopod slightly overreaching the distal margin of the antepenultimate segment and the fourth pereiopod with the exopod reaching beyond the end of the merus. Morphological data and a partial sequence of the 16S rRNA gene of the new species are provided to show its systematic position.


2016 ◽  
Vol 8 (14) ◽  
pp. 9619
Author(s):  
Amit Sayyed ◽  
Robert Alexander Pyron ◽  
Neelesh Dahanukar

Cnemaspis flaviventralis, a new species of day gecko, is described from the forests of Amboli, Sindhudurg District, Maharashtra State, northern Western Ghats, India.  The new species was previously confused with the sympatric species Cnemaspis girii, C. indraneildasii, C. kolhapurensis and C. goaensis.  It is distinguished from C. giri by having spine-like tubercles on flanks, granular dorsal scales intermixed with large, depressed, slightly keeled scales (vs. lack of spine-like tubercles on flanks, granular dorsal scales, intermixed with large smooth scales); from C. indraneildasii by having dorsal scales heterogeneous (vs. homogenous), lacking a series of enlarged median sub-caudal scales, and 28–29 (vs. 20) ventral scales across mid-body; from C. kolhapurensis by having heterogeneous (vs. homogenous) dorsal scalation, lacking spine-like tubercles on flanks and lacking pre-cloacal pores (vs. 24–28 pre-cloacal-femoral pores); and from C. goaensis by lacking pre-cloacal pores and lacking a series of enlarged median sub-caudal scales.  We further provide partial mitochondrial 16S rRNA gene sequences for the new species and for the sympatric species C. girii, C. kolhapurensis and C. goaensis, and show that the new species is genetically distinct.  


2020 ◽  
Vol 70 (3) ◽  
pp. 1769-1776 ◽  
Author(s):  
Ming Quan Lam ◽  
Maša Vodovnik ◽  
Maša Zorec ◽  
Sye Jinn Chen ◽  
Kian Mau Goh ◽  
...  

To date, there is sparse information for the genus Robertkochia with Robertkochia marina CC-AMO-30DT as the only described member. We report here a new species isolated from mangrove soil collected at Malaysia Tanjung Piai National Park and perform polyphasic characterization to determine its taxonomic position. Strain CL23T is a Gram-negative, yellow-pigmented, strictly aerobic, catalase-positive and oxidase-positive bacterium. The optimal growth conditions were determined to be at pH 7.0, 30–37 °C and in 1–2 % (w/v) NaCl. The major respiratory quinone was menaquinone-6 (MK-6) and the highly abundant polar lipids were four unidentified lipids, a phosphatidylethanolamine and two unidentified aminolipids. The 16S rRNA gene similarity between strain CL23T and R. marina CC-AMO-30DT is 96.67 %. Strain CL23T and R. marina CC-AMO-30DT clustered together and were distinguished from taxa of closely related genera in 16S rRNA gene phylogenetic analysis. Genome sequencing revealed that strain CL23T has a genome size of 4.4 Mbp and a G+C content of 40.72 mol%. Overall genome related indexes including digital DNA–DNA hybridization value and average nucleotide identity are 17.70 % and approximately 70%, below the cutoffs of 70 and 95%, respectively, indicated that strain CL23T is a distinct species from R. marina CC-AMO-30DT. Collectively, based on the phenotypic, chemotaxonomic, phylogenetic and genomic evidences presented here, strain CL23T is proposed to represent a new species with the name Robertkochia solimangrovi sp. nov. (KCTC 72252T=LMG 31418T). An emended description of the genus Robertkochia is also proposed.


2020 ◽  
Vol 70 (3) ◽  
pp. 1630-1638 ◽  
Author(s):  
Alexandra Pitt ◽  
Ulrike Koll ◽  
Johanna Schmidt ◽  
Martin W. Hahn

Strain 33A1-SZDPT was isolated from a small creek located in Puch, Austria. Strain SP-Ram-0.45-NSY-1T was obtained from a small pond located in Schönramer Moor, Germany. 16S rRNA gene sequence similarities between the type strain of Silvanigrella aquatica , currently the only member of the family Silvanigrellaceae , and strains 33A1-SZDPT and SP-Ram-0.45-NSY-1T of 94.1 and 99.1 %, respectively, suggested affiliation of the two strains with this family. Phylogenetic reconstructions with 16S rRNA gene sequences and phylogenomic analyses with amino acid sequences obtained from 103 single-copy genes suggested that the strains represent a new genus and a new species in the case of strain 33A1-SZDPT (=JCM 32978T=DSM 107810T), and a new species within the genus Silvanigrella in the case of strain SP-Ram-0.45-NSY-1T (=JCM 32975T=DSM 107809T). Cells of strain 33A1-SZDPT were motile, pleomorphic, purple-pigmented on agar plates, putatively due to violacein, and showed variable pigmentation in liquid media. They grew chemoorganotrophically and aerobically and tolerated salt concentrations up to 1.2 % NaCl (v/w). The genome size of strain 33A1-SZDPT was 3.4 Mbp and the G+C content was 32.2 mol%. For this new genus and new species, we propose the name Fluviispira multicolorata gen. nov., sp. nov. Cells of strain SP-Ram-0.45-NSY-1T were motile, pleomorphic, red-pigmented and grew chemoorganotrophically and aerobically. They tolerated salt concentrations up to 1.1 % NaCl (v/w). The genome size of strain SP-Ram-0.45-NSY-1T was 3.9 Mbp and the G+C content 29.3 mol%. For the new species within the genus Silvanigrella we propose the name Silvanigrella paludirubra sp. nov.


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