scholarly journals Fatty acids in component of milk enhance the expression of the cAMP-response-element-binding-protein-binding protein (CBP)/p300 gene in developing rats

2008 ◽  
Vol 99 (3) ◽  
pp. 481-486 ◽  
Author(s):  
Kazuki Mochizuki ◽  
Hiromi Kawai ◽  
Hiroko Mochizuki ◽  
Masaya Shimada ◽  
Sachiko Takase ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Small Intestine ◽  
Protein Binding ◽  
Binding Protein ◽  
Mrna Level ◽  
Camp Response Element Binding ◽  
Camp Response Element ◽  
Response Element ◽  
Element Binding Protein

Fatty acids in milk are thought to play an important role in intestinal maturation and gene expression in the rat small intestine during the suckling–weaning period. In the present study, we determined the jejunal mRNA level of the cAMP-response-element-binding-protein-binding protein (CBP)/p300, which is one of the chromatin remodelling factors and regulates histone acetylation, during the postnatal period in rats. The mRNA level of CBP/p300 was high during the suckling and middle of the weaning period (day 5 to 20) and then declined sharply to a low level at the end of the weaning period and after weaning. In situ hybridisation also showed that CBP/p300 mRNA levels in the villus as well as the basal membrane clearly decreased after weaning. Rat pups at age 17 d, weaned to a high-fat diet, showed higher levels of CBP/p300 mRNA than those weaned to a low-fat diet. Oral administration of caprylic acid, oleic acid and linoleic acid, which are major fatty acid components in milk, induced jejunal CBP/p300 gene expression. The present results suggest that fatty acids in components of milk enhance expression of the CBP/p300 genes in the small intestine.

scholarly journals Induction of Human NF-IL6β by Epidermal Growth Factor Is Mediated through the p38 Signaling Pathway and cAMP Response Element-binding Protein Activation in A431 Cells

2005 ◽  
Vol 16 (7) ◽  
pp. 3365-3376 ◽  
Author(s):  
Ju-Ming Wang ◽  
Joseph T. Tseng ◽  
Wen-Chang Chang
Keyword(s):  
Gene Expression ◽  
Binding Protein ◽  
Camp Response Element Binding ◽  
Binding Activity ◽  
A431 Cells ◽  
Base Pairs ◽  
Camp Response Element ◽  
Response Element ◽  
Element Binding Protein ◽  
Epidermal Growth

The CCAAT/enhancer binding protein δ (C/EBPδ, CRP3, CELF, NF-IL6β) regulates gene expression and plays functional roles in many tissues, such as in acute phase response to inflammatory stimuli, adipocyte differentiation, and mammary epithelial cell growth control. In this study, we examined the expression of human C/EBPδ (NF-IL6β) gene by epidermal growth factor (EGF) stimulation in human epidermoid carcinoma A431 cells. NF-IL6β was an immediate-early gene activated by the EGF-induced signaling pathways in cells. By using 5′-serial deletion reporter analysis, we showed that the region comprising the –347 to +9 base pairs was required for EGF response of the NF-IL6β promoter. This region contains putative consensus binding sequences of Sp1 and cAMP response element-binding protein (CREB). The NF-IL6β promoter activity induced by EGF was abolished by mutating the sequence of cAMP response element or Sp1 sites in the –347/+9 base pairs region. Both in vitro and in vivo DNA binding assay revealed that the CREB binding activity was low in EGF-starved cells, whereas it was induced within 30 min after EGF treatment of A431 cells. However, no change in Sp1 binding activity was found by EGF treatment. Moreover, the phosphatidylinositol 3 (PI3)-kinase inhibitor (wortmannin) and p38MAPK inhibitor (SB203580) inhibited the EGF-induced CREB phosphorylation and the expression of NF-IL6β gene in cells. We also demonstrated that CREB was involved in regulating the NF-IL6β gene transcriptional activity mediated by p38MAPK. Our results suggested that PI3-kinase/p38MAPK/CREB pathway contributed to the EGF activation of NF-IL6β gene expression.

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