scholarly journals Effects of conjugated linoleic acid on growth, non-specific immunity, antioxidant capacity, lipid deposition and related gene expression in juvenile large yellow croaker (Larmichthys crocea) fed soyabean oil-based diets

2013 ◽  
Vol 110 (7) ◽  
pp. 1220-1232 ◽  
Author(s):  
Rantao Zuo ◽  
Qinghui Ai ◽  
Kangsen Mai ◽  
Wei Xu
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Antioxidant Capacity ◽  
Fatty Acid Oxidation ◽  
Acid Oxidation ◽  
Large Yellow Croaker ◽  
Lipid Deposition ◽  
Related Gene ◽  
Specific Immunity ◽  
Acyl Coa Oxidase

The effects of conjugated linoleic acid (CLA) on growth performance, non-specific immunity, antioxidant capacity, lipid deposition and related gene expression were investigated in the large yellow croaker (Larmichthys crocea). Fish (7·56 (sem 0·60) g) were fed soyabean oil-based diets with graded levels of CLA (0, 0·42, 0·83, 1·70 %) for 70 d. Quantitative PCR was used to assess the effects of CLA on the transcription of inflammation- and fatty acid oxidation-related genes. Growth in fish fed the diet with 0·42 % CLA was significantly higher. Also, phagocytic index and respiratory burst activity were significantly higher in fish fed the diets containing 0·42 and 0·83 % CLA, respectively. Hepatic total antioxidative capacity and catalase activities increased significantly when CLA increased from 0 to 0·83 %, and then decreased with further increase of CLA. However, hepatic malondialdehyde content decreased significantly as dietary CLA increased. Lipid concentration in the whole body and muscle increased significantly with increasing dietary CLA. Transcription of genes related to inflammation (cyclo-oxygenase-2 and IL-β) in the liver and kidney and fatty acid oxidation (carnitine palmitoyl transferase I and acyl CoA oxidase) in the kidney decreased significantly as dietary CLA increased. PPARα and acyl CoA oxidase expression in the liver decreased significantly as CLA increased from 0·42 to 1·70 %. These results strongly suggest that dietary CLA could significantly affect growth performance, non-specific immunity, antioxidant capacity, lipid deposition and transcription of inflammation- and fatty acid oxidation-related genes of the large yellow croaker. This may contribute to our understanding of the mechanisms related to the physiological effects of dietary CLA in fish.

Fatty acid oxidation and related gene expression in heart depleted of carnitine by mildronate treatment in the rat

2004 ◽  
Vol 258 (1/2) ◽  
pp. 171-182 ◽  
Author(s):  
Pascal Degrace ◽  
Laurent Demizieux ◽  
Joseph Gresti ◽  
Marcelline Tsoko ◽  
Agnès André ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Acid Oxidation ◽  
Related Gene

scholarly journals Tang-Nai-Kang Alleviates Pre-diabetes and Metabolic Disorders and Induces a Gene Expression Switch toward Fatty Acid Oxidation in SHR.Cg-Leprcp/NDmcr Rats

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0122024 ◽  
Author(s):  
Linyi Li ◽  
Hisae Yoshitomi ◽  
Ying Wei ◽  
Lingling Qin ◽  
Jingxin Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Metabolic Disorders ◽  
Acid Oxidation

scholarly journals SIRT4 Regulates Fatty Acid Oxidation and Mitochondrial Gene Expression in Liver and Muscle Cells

2010 ◽  
Vol 285 (42) ◽  
pp. 31995-32002 ◽  
Author(s):  
Nargis Nasrin ◽  
Xiaoping Wu ◽  
Eric Fortier ◽  
Yajun Feng ◽  
Olivia Claire Bare' ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Mitochondrial Gene ◽  
Muscle Cells ◽  
Acid Oxidation ◽  
Mitochondrial Gene Expression

scholarly journals Comparison of the activities of some peroxisomal and extraperoxisomal lipid-metabolizing enzymes in liver and extrahepatic tissues of the rat

1985 ◽  
Vol 227 (3) ◽  
pp. 737-741 ◽  
Author(s):  
P Van Veldhoven ◽  
G P Mannaerts
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Acid Oxidation ◽  
Dihydroxyacetone Phosphate ◽  
Metabolizing Enzymes ◽  
Mitochondrial Fatty Acid ◽  
Lipid Metabolizing Enzymes ◽  
Extrahepatic Tissues ◽  
Glycerolipid Synthesis ◽  
Acyl Coa Oxidase

Peroxisomal (acyl-CoA oxidase and peroxisomal dihydroxyacetone-phosphate acyltransferase) and extraperoxisomal (mitochondrial fatty acid oxidation, extraperoxisomal dihydroxyacetone-phosphate acyltransferase, mitochondrial and microsomal glycerophosphate acyltransferases) lipid-metabolizing enzymes were measured in homogenates from rat liver and from seven extrahepatic tissues. Except for jejunal mucosa and kidney, extrahepatic tissues contained very little acyl-CoA oxidase activity. Peroxisomal dihydroxyacetone-phosphate acyltransferase, taken as the activity that was not inhibited by 5 mM-glycerol 3-phosphate, was present in all tissues examined, and its specific activity in liver and extrahepatic tissues was roughly of the same order of magnitude. Clofibrate treatment increased the activity of acyl-CoA oxidase in liver, and to a smaller extent also in kidney, but did not influence the activity of peroxisomal dihydroxyacetone-phosphate acyltransferase. Comparison of the activities of peroxisomal and extraperoxisomal lipid-metabolizing enzymes in extrahepatic tissues and in liver, an organ in which the contribution of peroxisomes to fatty acid oxidation and to glycerolipid synthesis has been estimated previously, suggests that, as in liver, peroxisomal long-chain fatty acid oxidation is of minor quantitative importance in extrahepatic tissues, but that in these tissues (micro)-peroxisomes are responsible for most of the dihydroxyacetone phosphate acylation and, consequently, for initiating ether glycerolipid synthesis.

scholarly journals Gene Expression Associated with Apple Aroma Biosynthesis

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 977C-977
Author(s):  
Nobuko Sugimoto ◽  
Steve van Nocker ◽  
Schuyler Korban ◽  
Randy Beaudry
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Ethylene Biosynthesis ◽  
Acid Oxidation ◽  
Beta Oxidation ◽  
Ripening Stages ◽  
Ester Formation ◽  
Ester Biosynthesis ◽  
Marked Decline ◽  
Acyl Coa Oxidase

A microarray containing over 10,000 gene fragments was used to link changes in gene expression with changes in aroma biosynthesis in ripening apple (Malus ×domestica Borkh). The microarray was probed with fluorescent-tagged cDNA derived from RNA extracted from `Jonagold' apple skin and cortex tissue representing eight distinct physiological stages spanning 70 days during ripening and senescence. The ripening stages, in chronological order, were: 1) early preclimacteric; 2) late preclimacteric and onset of trace ester biosynthesis; 3) onset of the autocatalytic ethylene and rapidly increasing ester biosynthesis; 4) half-maximal ester biosynthesis and engagement of the respiratory climacteric; 5) near maximal ester biosynthesis, peak in respiratory activity, and the onset of rapid tissue softening; 6) maximal ester biosynthesis prior to its decline, the conclusion of the respiratory climacteric, and the completion of tissue softening; 7) midpoint in the decline in ester biosynthesis and maximal ethylene biosynthesis; and 8) postclimacteric minimum in ester production. Patterns in gene expression reflecting the rise and fall in ester formation were found in some putative genes for beta-oxidation (acyl-CoA oxidase, enoyl-CoA hydratase, and acetyl-CoA acetyl transferase), ester formation (aminotransferase, alcohol dehydrogenase, and alcohol acyl transferase), and fatty acid oxidation (lipoxygenase), but not fatty acid biosynthetic genes. A marked decline coinciding with the onset of ester production was detected in several putative genes for ADH.

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