scholarly journals Population genetics of the brown marmorated stink bug Halyomorpha halys in the early phase of invasion in South Tyrol (Northern Italy)

2020 ◽  
pp. 1-8
Author(s):  
Hannes Schuler ◽  
David Elsler ◽  
Stefanie Fischnaller
Keyword(s):  
Haplotype Diversity ◽  
Northern Italy ◽  
Small Scale ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Invasion Pathways ◽  
Long Distance ◽  
South Tyrol ◽  
Invasion Pathway

Abstract The brown marmorated stink bug Halyomorpha halys is one of the most harmful invasive species in the world. Native to East Asia, this insect was introduced into North America in the 1990s and into Europe in the 2000s where it subsequently established and spread across the continent. Previous population genetic studies determined the invasion pathways at continental and national levels. However, information on the dynamics on a small-scale is currently scarce. Here we study the genetic diversity and population dynamics of H. halys in South Tyrol, a region in Northern Italy, since its arrival to its widespread establishment over a period of four years. By haplotyping 162 individuals from ten populations (including six previously published individuals) we found a high haplotype diversity in most populations with an increasing diversity across the different years. Most haplotypes were previously found in other regions of Northern Italy, providing evidence for migration from neighboring regions. However, the presence of four previously undescribed haplotypes as well as a haplotype previously found exclusively in Greece highlights additional long-distance dispersal across the continent. Phylogenetic analysis of the haplotypes found in South Tyrol showed that the majority of haplotypes clustered with haplotypes predominantly found in Japan. This suggests a potential recent introduction of H. halys individuals from Japan into Europe, and thus an additional invasion pathway that was previously unidentified.

scholarly journals Co-haplotyping symbiont and host to unravel invasion pathways of the exotic pest Halyomorpha halys in Italy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Isabel Martinez-Sañudo ◽  
M. Alejandra Perotti ◽  
Davide Scaccini ◽  
Alberto Pozzebon ◽  
Laura Marri ◽  
...  
Keyword(s):  
Mitochondrial Gene ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Stink Bug ◽  
Invasion Pathways ◽  
Association Pattern ◽  
The North ◽  
North Eastern ◽  
Important Pest

Abstract The brown marmorated stink bug Halyomorpha halys (Stål) is a globally invasive species that harbours the primary bacterial symbiont ‘Candidatus Pantoea carbekii’. In this work, P. carbekii was used as another genetic marker to investigate the biodiversity and biogeographical patterns of this important pest, in native and newly invaded areas, especially in Italy. The correlation between the genetic structure of the symbiont and that of its host was studied through the analyses of one bacterial and one host marker, the putative pseudogene ΔybgF and the mitochondrial gene COI, respectively. As a result, five new P. carbekii haplotypes were identified, and an association pattern between host-symbiont haplotypes was observed. Host species showed higher haplotype diversity than symbiont, which can be expected in a long term host-symbiont association. Populations from the north-eastern Italy showed the highest values of genetic diversity for both markers, highlighting that this particular Italian area could be the result of multiple ongoing introductions. Moreover, some of the symbiont-host haplotypes observed were shared only by populations from north-eastern Italy and native areas, especially Japan, suggesting further introductions from this native country to Italy. Overall, our findings improve the understanding of the potential origin of multiple accidental introductions of H. halys in Italy.

scholarly journals Genetic diversity analysis of brown marmorated stink bug, Halyomorpha halys based on mitochondrial COI and COII haplotypes

2020 ◽  
Author(s):  
Juncong Yan ◽  
Chandan Pal ◽  
Diane Anderson ◽  
Gábor Vétek ◽  
Péter Farkas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Difference ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
China And Japan

Abstract Background: In the past decade, the brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge on and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries. Result: In total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity (Hd) and nucleotide diversity (π) were high while the neutrality (Fu's Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu's Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu's Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exist between groups of populations: China, Japan, and the invasive populations. Conclusion: This study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred and a new stable genetic cluster has established in Europe and the USA. The study also revealed that three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies, especially biocontrol.

scholarly journals Genetic diversity analysis of brown marmorated stink bug, Halyomorpha halys based on mitochondrial COI and COII haplotypes

2020 ◽  
Author(s):  
Juncong Yan ◽  
Chandan Pal ◽  
Diane Anderson ◽  
Gábor Vétek ◽  
Péter Farkas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Difference ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
China And Japan

Abstract Background: In the past decade, the brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge on and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries.Result: In total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity (Hd) and nucleotide diversity (π) were high while the neutrality (Fu's Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu's Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu's Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exist between groups of populations: China, Japan, and the invasive populations.Conclusion: This study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred and a new stable genetic cluster has established in Europe and the USA. The study also revealed that three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies, especially biocontrol.

scholarly journals Genetic Diversity Analysis of Brown Marmorated Stink Bug, Halyomorpha Halys Based on Mitochondrial COI and COII Haplotypes

2020 ◽  
Author(s):  
Juncong Yan ◽  
Chandan Pal ◽  
Diane Anderson ◽  
Gábor Vétek ◽  
Péter Farkas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Difference ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
China And Japan

Abstract Background:In the past decade, the brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge on and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries.Result:In total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity (Hd) and nucleotide diversity (π) were high while the neutrality (Fu's Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu's Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu's Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exist between groups of populations: China, Japan, and the invasive populations.Conclusion:This study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred and a new stable genetic cluster has established in Europe and USA. The study also revealed that three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies, especially biocontrol.

scholarly journals First evidence of Halyomorpha halys (Stål) (Hemiptera Heteroptera, Pentatomidae) feeding on rice (Oryza sativa L.)

2017 ◽  
Vol 49 (1) ◽  
Author(s):  
D. Lupi ◽  
P. Dioli ◽  
L. Limonta
Keyword(s):  
Oryza Sativa ◽  
Host Plant ◽  
Oryza Sativa L ◽  
Northern Italy ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Perennial Crops ◽  
Heteroptera Pentatomidae

The brown marmorated stink bug, <em>Halyomorpha</em> <em>halys</em> (Stål) is a pest of numerous annual and perennial crops. Additional distribution records for <em>H. halys</em> are provided from northern Italy where rice is cultivated, and the presence of adults feeding on panicles gives the first evidence of an association between this pest and rice (<em>Oryza sativ</em>a L.), a crop not previously recorded as a host plant.

scholarly journals Genetic diversity analysis of brown marmorated stink bug, Halyomorpha halys based on mitochondrial COI and COII haplotypes

2020 ◽  
Author(s):  
Juncong Yan ◽  
Chandan Pal ◽  
Diane Anderson ◽  
Gábor Vétek ◽  
Péter Farkas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Difference ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
China And Japan

Abstract Background: In the past decade, the brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries. Result: In total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity ( Hd ) and nucleotide diversity ( π ) were high while the neutrality (Fu's Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu's Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu's Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exists among the BMSB populations from China, Japan, and the invasive countries. Conclusion: This study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred in Europe and the USA. The study also revealed three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies.

scholarly journals Diversity and distribution of cytochrome oxidase I (COI) haplotypes of the brown marmorated stink bug, Halyomorpha halys Stål (Hemiptera, Pentatomidae), along the eastern front of its invasive range in Eurasia

NeoBiota ◽  
2021 ◽  
Vol 68 ◽  
pp. 53-77
Author(s):  
Tara D. Gariepy ◽  
Dmitry L. Musolin ◽  
Aleksandra Konjević ◽  
Natalia N. Karpun ◽  
Vilena Y. Zakharchenko ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome Oxidase ◽  
Haplotype Diversity ◽  
Cytochrome Oxidase I ◽  
Future Research ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Invasive Range ◽  
Eastern Front

The arrival, establishment and pest status of Halyomorpha halys in Europe and non-native countries in Asia have been well-documented, with thorough characterisation of the genetic diversity and occurrence of cytochrome oxidase I (COI) haplotypes in Switzerland, France, Hungary, Italy and Greece. However, a number of gaps exist in terms of the characterisation of the haplotype diversity and occurrence of H. halys along the invasion front that covers eastern Europe, western and central Asia. To contribute towards filling this gap, the COI haplotype diversity and distribution were investigated for H. halys collected in Serbia, Ukraine, Russia, Georgia and Kazakhstan. A total of 646 specimens were analysed and five haplotypes were found (H1, H3, H8, H33 and H80). Haplotype H1 was present in all five countries investigated and was the only haplotype detected amongst &gt; 500 specimens collected from Ukraine, Russia and Georgia. H1 (82%) was the dominant haplotype found in Kazakhstan, alongside H3 (18%). In contrast to the low or no diversity observed in these four countries, Serbia had higher haplotype diversity and was represented by five haplotypes. Although H3 was dominant (47%) in Serbia, H1 was also prevalent (40%); the remaining haplotypes (H8, H33 and H80) were minor contributors (1–11%) to the haplotype composition. The results are discussed in context with other known populations in neighbouring countries and patterns of haplotype diversity indicate the movement of successful invasive populations in Europe to generate secondary invasions along the eastern front of the invasion in Eurasia. Possible scenarios regarding the spread of particular haplotypes in these regions are discussed, along with suggestions for future research to fill existing gaps.

scholarly journals Genetic diversity analysis of brown marmorated stink bug, Halyomorpha halys based on mitochondrial COI and COII haplotypes

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Juncong Yan ◽  
Chandan Pal ◽  
Diane Anderson ◽  
Gábor Vétek ◽  
Péter Farkas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Genetic Difference ◽  
Management Strategies ◽  
Haplotype Diversity ◽  
Halyomorpha Halys ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
China And Japan

Abstract Background In the past decade, the brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) has caused extensive damage to global agriculture. As a high-risk pest for many countries, including New Zealand, it is important to explore its genetic diversity to enhance our knowledge and devise management strategies for BMSB populations. In this study, two mitochondrial genes, Cytochrome c oxidase I (COI) and Cytochrome c oxidase II (COII) were used to explore the genetic diversity among 463 BMSB individuals collected from 12 countries. Result In total, 51 COI and 29 COII haplotypes of BMSB were found, which formed 59 combined haplotypes (5 reported and 54 novel). Of these, H1h1 was the predominant haplotype. The haplotype diversity (Hd) and nucleotide diversity (π) were high while the neutrality (Fu’s Fs) values were negative for the BMSB populations in the native countries, China, and Japan. For the BMSB populations from the invaded countries, the Fu’s Fs values were negative for populations from Chile, Georgia, Hungary, Italy, Romania, Turkey, and USA, indicating that those populations are under demographic expansion. In comparison, the Fu’s Fs values were positive for the populations from Austria, Serbia, and Slovenia, revealing a potential population bottleneck. Analysis of molecular variance (AMOVA) suggested that significant genetic difference exists among the BMSB populations from China, Japan, and the invasive countries. Conclusion This study revealed that the haplotype diversity of the BMSB populations was high in those two studied countries where BMSB is native to (China and Japan) but low in those countries which have been invaded by the species. The analysis indicated that multiple invasions of BMSB occurred in Europe and the USA. The study also revealed three ancestral lines and most of the novel haplotypes were evolved from them. Moreover, we observed two genetic clusters in the invasive populations that are formed during different invasion events. Our study provided a comprehensive overview on the global haplotypes distribution thus expanding the existing knowledge on BMSB genetic diversity that potentially could play an important role in formulating feasible pest management strategies.

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