scholarly journals Linkage map of random amplified polymorphic DNAs (RAPDs) in the silkworm, Bombyx mori

1995 ◽  
Vol 66 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Amornrat Promboon ◽  
Toru Shimada ◽  
Haruhiko Fujiwara ◽  
Masahiko Kobayashi
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Mapping Function ◽  
Mendelian Inheritance ◽  
Linkage Groups ◽  
Lod Score ◽  
Chi Square ◽  
Segregation Ratios ◽  
Chi Square Test ◽  
Silkworm Bombyx Mori

SummaryWe have constructed a linkage map of random amplified polymorphic DNAs (RAPDs) in Bombyx mori. We screened 320 10-mer primers, and found 243 clear polymorphic bands between C108 and p50 strains. In the F2 generation, segregation ratios of 168 bands were nearly 3:1 in a chi square test, showing Mendelian inheritance. The MAPMAKER program sorted 168 bands into 29 linkage groups and 10 unlinked loci at minimum LOD score 3·0, and determined orders of loci in each group, which contained 2–11 markers. It also detected typing errors in our data. We calculated map distances between pairs of neighbouring loci using recombination values in males and the Kosambi mapping function. Our RAPD map consists of 169 loci including the p locus, and the sum of map distances is approximately 900 cM. Linkage groups 1 and 2 of our map correspond to chromosomes 1 and 2 on the conventional linkage map because of linkage to sex and p, respectively.

Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28)

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 626-629 ◽  
Author(s):  
Yuji Yasukochi ◽  
Yutaka Banno ◽  
Kohji Yamamoto ◽  
Marian R Goldsmith ◽  
Hiroshi Fujii
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Analysis ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Linkage Maps ◽  
Wild Type ◽  
Linkage Groups ◽  
Sequence Tagged Sites ◽  
Silkworm Bombyx Mori

Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was carried out using BF1 progeny between wild-type and mutant stocks carrying morphological phenotypic markers. As a result, the counterparts for 26 of 28 molecular LGs were identified with their counterparts of the classical LGs. Two visible markers, Sel and Xan, representing different classical LGs, were found to be linked.Key words: Bombyx mori, classical linkage group (LG), PCR-based genotyping, mutant, STS.

scholarly journals A Dense Genetic Map of the Silkworm, Bombyx mori, Covering All Chromosomes Based on 1018 Molecular Markers

Genetics ◽  
1998 ◽  
Vol 150 (4) ◽  
pp. 1513-1525 ◽  
Author(s):  
Yuji Yasukochi
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Markers ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Genetic Markers ◽  
Genetic Map ◽  
Z Chromosome ◽  
Linkage Groups ◽  
Average Interval ◽  
Silkworm Bombyx Mori

Abstract A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering ∼2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was ∼2 cM, equal to ∼500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.

scholarly journals A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms

1995 ◽  
Vol 66 (2) ◽  
pp. 109-126 ◽  
Author(s):  
Jinrui Shi ◽  
David G. Heckel ◽  
Marian R. Goldsmith
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Genetic Maps ◽  
Crossing Over ◽  
Linkage Groups ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

SummaryWe present data for the initial construction of a molecular linkage map for the domesticated silkworm, Bombyx mori, based on 52 progeny from an F2 cross from a pair mating of inbred strains p50 and C108, using restriction fragment length polymorphisms (RFLPs). The map contains 15 characterized single copy sequences, 36 anonymous sequences derived from a follicular cDNA library, and 10 loci corresponding to a low copy number retrotransposon, mag. The 15 linkage groups and 8 ungrouped loci account for 23 of the 28 chromosomes and span a total recombination length of 413 cM; 10 linkage groups were correlated with established classic genetic maps. Scoring data from Southern blots were analysed using two Pascal programs written specifically to analyse linkage data in Lepidoptera, where females are the heterogametic sex and have achiasmatic meiosis (no crossing-over). These first examine evidence for linkage by calculating the maximum lod score under the hypothesis that the two loci are linked over the likelihood under the hypothesis that the two loci assort independently, and then determine multilocus linkage maps for groups of putatively syntenic loci by calculating the maximum likelihood estimate of the recombination fractions and the log likelihood using the EM algorithm for a specified order of loci along the chromosome. In addition, the possibility of spurious linkage was exhaustively tested by searching for genotypes forbidden by the absence of crossing-over in one sex.

scholarly journals Molecular Mapping of Capsicum and the Comparisons of Synteny with Tomato

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 615e-615
Author(s):  
Yiping Zhang ◽  
Vince K. Lackney ◽  
James P. Prince ◽  
Jim P. Blauth ◽  
Molly M. Kyle
Keyword(s):  
Mosaic Virus ◽  
Molecular Mapping ◽  
Basic Chromosome Number ◽  
Linkage Groups ◽  
Chi Square ◽  
Length Polymorphisms ◽  
Monogenic Resistance ◽  
Segregation Ratios ◽  
Solanaceous Plant ◽  
Two Populations

Pepper (Capsicum spp.) has the same basic chromosome number as tomato and other solanaceous plant species (2n = 2x = 24). By using the probes mostly from a tomato map, we have generated three molecular maps of pepper from interspecific F2 crosses of C. frutescens BG 2814-6, C. chinense PI 159234 and C. annuum `NuMex RNaky' with restriction fragment length polymorphisms, isozymes, random amplified polymorphic DNAs, and morphological traits. The best developed map is from C. annuum × C. chinense F2 cross, which currently has 366 markers covered 1081 cM in 18 linkage groups. Three linkage groups were assigned to three chromosomes based on primary trisomics. Several disease resistance genes including monogenic resistance to potyviruses and quantitative trait loci for resistance to tobacco mosaic virus and cucumber mosaic virus have been mapped. The distribution of allele frequency and marker segregation ratios have been analyzed. Chi-square analyses of all clones showed more skewing of segregation ratios in C. annuum × C. chinense population than the other two populations. The skewing occurs throughout the genome and tends towards heterozygote and one of the parents. The order of markers among three pepper maps will be compared and the comparisons of synteny between pepper and tomato maps will be described. A composite of three pepper maps will be presented using JoinMap software.

scholarly journals Inheritance of resistance to powdery mildew in pea and pathogenesis-related aspects

2012 ◽  
Vol 38 (2) ◽  
pp. 119-122
Author(s):  
Ricardo Lima dos Santos ◽  
Marcelo Agenor Pavan ◽  
Norberto da Silva ◽  
Leandro Augusto Andrade Fumes
Keyword(s):  
Powdery Mildew ◽  
Inheritance Of Resistance ◽  
Chi Square ◽  
Cellular Death ◽  
Inheritance Study ◽  
Pathogenesis Related ◽  
Segregation Ratios ◽  
Histological Data ◽  
Chi Square Test ◽  
Susceptible Populations

The inheritance of resistance to powdery mildew in the pea cultivar MK-10 and some histological aspects of infection were assessed. For the inheritance study, F1, F2, backcrosses and F3 generations of MK-10 crossed with two susceptible populations were evaluated. Histological evaluations included percentage of germinated conidia, percentage of conidia that formed appresoria, percentage of conidia that established colonies, and number of haustoria per colony. Segregation ratios obtained in the resistance inheritance study were compared by Chi-square (ײ) test and the histological data were analyzed by Tukey's test at 5% probability. It was concluded that resistance of MK-10 to powdery mildew is due to a pair of recessive alleles since it is expressed in the pre-penetration stage and completed by post-penetration localized cellular death, characteristic of the presence of the pair of recessive alleles er1er1.

scholarly journals A BAC-based integrated linkage map of the silkworm Bombyx mori

2008 ◽  
Vol 9 (1) ◽  
pp. R21 ◽  
Author(s):  
Kimiko Yamamoto ◽  
Junko Nohata ◽  
Keiko Kadono-Okuda ◽  
Junko Narukawa ◽  
Motoe Sasanuma ◽  
...  
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Integrated Linkage Map ◽  
Silkworm Bombyx Mori

Construction and analysis of an AFLP molecular linkage map of the silkworm (Bombyx mori)

2006 ◽  
Vol 3 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Sima Yang-Hu ◽  
Li Bin ◽  
Chen Da-Xia ◽  
Sun De-Bin ◽  
Zhao Ai-Chun ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Average Length ◽  
Average Distance ◽  
Linkage Groups ◽  
Molecular Linkage Map ◽  
Average Variation ◽  
The Mean ◽  
Marker Distance

AbstractAn amplification fragment length polymorphism (AFLP) molecular linkage map with a relatively high density for location of quantitative trait loci (QTLs) controlling the quantitative traits of silkworm (Bombyx mori) cocoons, was constructed using 91 individuals of the F2 generation. Among the 692 effective loci, 550 were allocated to subgroups a and b, of which 21 linkage groups in subgroup a had 233 molecular markers and 28 linkage groups in subgroup b had 317 markers. The number of markers on each linkage group in subgroups a and b ranged from 4 to 43 and 3 to 35, respectively. The total length of linkage groups for subgroup a was 1868.10 cM, and 2677.50 cM for subgroup b. The length of linkage group varied from 22.3 to 424.3 cM in subgroup a and from 2.4 to 366.5 cM in subgroup b. The average variation in the distance between markers was 3.39–17.43 cM in subgroup a and 0.8–26.96 cM in subgroup b. The average distance between the markers was 8.81 cM in subgroup a and 9.26 cM in subgroup b. There were 14 linkage groups, with an average distance below 10 cM, in subgroup a and 18 linkage groups in subgroup b. There were seven linkage groups with an average distance between 10 and 20 cM in subgroup a and ten linkage groups in subgroup b. Each linkage group in subgroup a had 11.1 loci on average, while there were 11.31 loci on each linkage group on average in subgroup b. The mean length for linkage groups in subgroups a and b was 89 and 95.6 cM, respectively. The total average length for both a and b subgroups was 2272.8, and 9.06 cM for the average marker distance, fulfilling the basic known requirements for locating QTLs.

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