Chick rearing: VIII. The preparation of a sensitive and diagnostic stained antigen for the rapid whole-blood test for bacillary white diarrhoea of chicks

1945 ◽  
Vol 35 (4) ◽  
pp. 232-235
Author(s):  
T. Barton Mann
Keyword(s):  
Growth Factors ◽  
Whole Blood ◽  
Blood Test ◽  
Laboratory Work ◽  
Keen Interest ◽  
Anaerobic Organism ◽  
Growth Requirements

A method is presented for the preparation of a sensitive and diagnostic stained antigen for the detection of fowls suspected of infection by the organisms found in epidemic bacillary white diarrhoea (pullorum disease).Evidence is presented that an antigen prepared from organisms grown in a medium which provides for the peculiar growth requirements of the anaerobic organism associated with anaerogenic pullorum, is more sensitive than antigens prepared from organisms grown on media which lack these peculiar growth factors.A stained antigen has been prepared which is agglutinated by pullorum antisera in 15 sec. at 4°C. and in 6 sec. a t 15°C. and which is not agglutinated by normal sera in 5 min.The author wishes to thank the Directors of Messrs J. Bibby and Sons, Ltd., for permission to publish these papers.In particular he would thank Mr J. Pye Bibby for his keen interest in the investigations and for his loyal support throughout.In addition he wishes to thank Miss Margaret Davies for her assistance in the bacteriological investigations, and Miss Margaret Shafto for laboratory work and the compilation of relevant data.

Forensic Electrochemical Presumptive Blood Test Based on the Voltammetric Behaviour of Methylene Blue and Whole Blood

2021 ◽  
Author(s):  
Sarah Cook ◽  
Kevin C. Honeychurch
Keyword(s):  
Methylene Blue ◽  
Whole Blood ◽  
Blood Test ◽  
Archaeological Sciences

The ability to identify the presence of blood residues is important in a number of fields, such as in the forensic and archaeological sciences. A number of tests presently exist;...

scholarly journals Diagnostic accuracy of a rapid whole-blood test for detection of Helicobacter pylori.

1997 ◽  
Vol 35 (10) ◽  
pp. 2695-2697 ◽  
Author(s):  
H Enroth ◽  
R Rigo ◽  
K Hultén ◽  
L Engstrand
Keyword(s):  
Helicobacter Pylori ◽  
Diagnostic Accuracy ◽  
Whole Blood ◽  
Blood Test

Platelet-Rich Plasma Powder: A New Preparation Method for the Standardization of Growth Factor Concentrations

2016 ◽  
Vol 45 (4) ◽  
pp. 954-960 ◽  
Author(s):  
Matthias Kieb ◽  
Frank Sander ◽  
Cornelia Prinz ◽  
Stefanie Adam ◽  
Anett Mau-Möller ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Clinical Application ◽  
Whole Blood ◽  
Sports Medicine ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Transforming Growth Factor Β1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tgf Β1

Background: Platelet-rich plasma (PRP) is widely used in sports medicine. Available PRP preparations differ in white blood cell, platelet, and growth factor concentrations, making standardized research and clinical application challenging. Purpose: To characterize a newly standardized procedure for pooled PRP that provides defined growth factor concentrations. Study Design: Controlled laboratory study. Methods: A standardized growth factor preparation (lyophilized PRP powder) was prepared using 12 pooled platelet concentrates (PCs) derived from different donors via apheresis. Blood samples and commercially available PRP (SmartPrep-2) served as controls (n = 5). Baseline blood counts were analyzed. Additionally, single PCs (n = 5) were produced by standard platelet apheresis. The concentrations of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), platelet-derived growth factor AB (PDGF-AB), transforming growth factor β1 (TGF-β1), insulin-like growth factor 1 (IGF-1), interleukin (IL)–1α, IL-1β, and IL-1 receptor agonist (IL-1RA) were analyzed by enzyme-linked immunosorbent assay, and statistical analyses were performed using descriptive statistics, mean differences, 95% CIs, and P values (analysis of variance). Results: All growth factor preparation methods showed elevated concentrations of the growth factors VEGF, bFGF, PDGF-AB, and TGF-β1 compared with those of whole blood. Large interindividual differences were found in VEGF and bFGF concentrations. Respective values (mean ± SD in pg/mL) for whole blood, SmartPrep-2, PC, and PRP powder were as follows: VEGF (574 ± 147, 528 ± 233, 1087 ± 535, and 1722), bFGF (198 ± 164, 410 ± 259, 151 ± 99, and 542), PDGF-AB (2394 ± 451, 17,846 ± 3087, 18,461 ± 4455, and 23,023), and TGF-β1 (14,356 ± 4527, 77,533 ± 13,918, 68,582 ± 7388, and 87,495). IGF-1 was found in SmartPrep-2 (1539 ± 348 pg/mL). For PC (2266 ± 485 pg/mL), IGF-1 was measured at the same levels of whole blood (2317 ± 711 pg/mL) but was not detectable in PRP powder. IL-1α was detectable in whole blood (111 ± 35 pg/mL) and SmartPrep-2 (119 ± 44 pg/mL). Conclusion: Problems with PRP such as absent standardization, lack of consistency among studies, and black box dosage could be solved by using characterized PRP powder made by pooling and lyophilizing multiple PCs. The new PRP powder opens up new possibilities for PRP research as well as for the treatment of patients. Clinical Relevance: The preparation of pooled PRP by means of lyophilization may allow physicians to apply a defined amount of growth factors by using a defined amount of PRP powder. Moreover, PRP powder as a dry substance with no need for centrifugation could become ubiquitously available, thus saving time and staff resources in clinical practice. However, before transferring the results of this basic science study to clinical application, regulatory issues have to be cleared.

Effects of Aspirin on Growth Factor Release From Freshly Isolated Leukocyte-Rich Platelet-Rich Plasma in Healthy Men: A Prospective Fixed-Sequence Controlled Laboratory Study

2019 ◽  
Vol 47 (5) ◽  
pp. 1223-1229 ◽  
Author(s):  
Prathap Jayaram ◽  
Peter Yeh ◽  
Shiv J. Patel ◽  
Racel Cela ◽  
Theodore B. Shybut ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Whole Blood ◽  
Low Dose ◽  
Platelet Rich Plasma ◽  
Healthy Men ◽  
Growth Factor Release ◽  
Tgf Β1 ◽  
Cox 1 ◽  
Factor Release

Background: The benefits of platelet-rich plasma (PRP) are believed to be in part dependent on growth factor release after platelet activation. Platelet activation is complex and involves multiple mechanisms. One important mechanism is driven by cyclooxygenase 1 (COX-1)–mediated conversion of arachidonic acid (AA) to precursor prostaglandins that then mediate proinflammatory responses that trigger growth factor release. Acetylsalicylic acid (ASA; also known as aspirin) is known to irreversibly inhibit COX-1, thereby blocking AA-mediated signaling; however, it is unclear whether ASA use alters growth factor release from freshly isolated PRP. Purpose: To assess the effects of low-dose ASA use on activation of growth factor release from freshly isolated human PRP via AA and thrombin (TBN). Study Design: Controlled laboratory study. Methods: Twelve healthy men underwent blood collection and leukocyte-rich PRP (LR-PRP) preparation through a double-spin protocol to obtain baseline whole blood and PRP counts the same day. PRP was aliquoted into 3 groups: nonactivated, AA activated, and TBN activated. Immediately after activation, the concentrations of transforming growth factor β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor AB (PDGF-AB) were measured using enzyme-linked immunosorbent assays (ELISAs). The same 12 participants were then placed on an 81-mg daily dose of oral ASA for 14 days. Repeat characterization of whole blood and PRP analyses was done on day 14, followed by repeat ELISAs of growth factors under the same nonactivated and activated settings as previously stated. Results: Fourteen days of daily ASA had no effect on the number of platelets and leukocytes measured in whole blood and LR-PRP. Compared with nonactivated LR-PRP, AA- and TBN-mediated activation led to significant release of VEGF and PDGF-AB. In contrast, release of TGF-β1 from LR-PRP was observed only with activation by AA, not with TBN. Consistent with its inhibitory role in AA signaling, ASA significantly inhibited AA-mediated release of all 3 growth factors measured in this study. Although ASA had no effect on TBN-mediated release of VEGF and TGF-β1 from LR-PRP, ASA did partially block TBN-mediated release of PDGF-AB, although the mechanism remains unclear. Conclusion: Daily use of low-dose ASA reduces VEGF, PDGF-AB, and TGF-β1 expression in freshly isolated human LR-PRP when activated with AA. Clinical Relevance: Reduction in growth factor release attributed to daily use of low-dose ASA or other COX inhibitors can be mitigated when PRP samples are activated with TBN. Clinical studies are needed to determine whether activation before PRP injection is needed in all applications where ASA is in use and to what extent ASA may inhibit growth factor release in vivo at the site of injury.

scholarly journals Validation of a rapid whole blood test for diagnosing Helicobacter pylori infection

BMJ ◽  
1997 ◽  
Vol 314 (7095) ◽  
pp. 1688-1688 ◽  
Author(s):  
A. Duggan ◽  
R. Logan ◽  
R J Robinson ◽  
M A Stone ◽  
J F Mayberry ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Whole Blood ◽  
Blood Test ◽  
Helicobacter Pylori Infection

scholarly journals Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen

2020 ◽  
Vol 9 (4) ◽  
pp. 1099
Author(s):  
Giorgio Serafini ◽  
Mariangela Lopreiato ◽  
Marco Lollobrigida ◽  
Luca Lamazza ◽  
Giulia Mazzucchi ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Whole Blood ◽  
Transforming Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Bone Morphogenetic Protein 2 ◽  
Time Points ◽  
Cell Counts ◽  
Tgf Β1

Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-β1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection; T7, 7 days; T14, 14 days). The cellular content of the liquid fibrinogen and whole blood was also calculated for each volunteer. A mean accumulation of platelets of almost 1.5-fold in liquid fibrinogen compared to whole blood samples was found. An increase of TGF-β1, PDGF-AB, FGF-2, and VEGF levels was detected at T7. At T14, the level of TGF-β1 returned to T0 level; PDGF-AB amount remained high; the levels of FGF-2 and VEGF decreased with respect to T7, but remained higher than the T0 levels; PDGF-BB was high at all time points; BMP-2 level was low and remained constant at all time points. TGF-β1, PDGF-AB, and PDGF-BB showed a correlation with platelet amount, whereas BMP-2, FGF-2, and VEGF showed a mild correlation with platelet amount. Due to the high concentration of platelets, liquid fibrinogen does contain important growth factors for the regeneration of both soft and hard tissue. The centrifugation protocol tested in this study provides a valid solution to stimulate wound healing in oral and periodontal surgery.

scholarly journals A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients

2020 ◽  
Author(s):  
Linda Petrone ◽  
Elisa Petruccioli ◽  
Valentina Vanini ◽  
Gilda Cuzzi ◽  
Saeid Najafi Fard ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Test ◽  
Specific Response

A Native whole Blood Test for the Evaluation of Blood-surface Interaction: Determination of Thromboxane Production

1984 ◽  
Vol 7 (3) ◽  
pp. 147-150 ◽  
Author(s):  
E. Mantovani ◽  
W. Marconi ◽  
B. Cebo ◽  
A.R. Togna ◽  
G. Togna ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Test ◽  
Surface Interaction ◽  
Thromboxane Production

Utility of a rapid whole blood test for an initial diagnosis of Helicobacter pylori infection

1998 ◽  
Vol 114 ◽  
pp. A164
Author(s):  
I. Kääriäinen ◽  
E. Pulkkinen ◽  
K. Romanoff ◽  
P. Sipponen ◽  
L. Sellin
Keyword(s):  
Helicobacter Pylori ◽  
Whole Blood ◽  
Blood Test ◽  
Initial Diagnosis ◽  
Helicobacter Pylori Infection

scholarly journals Role of serum components in density-dependent inhibition of growth of cells in culture. Platelet-derived growth factor is the major serum determinant of saturation density

1980 ◽  
Vol 85 (2) ◽  
pp. 377-385 ◽  
Author(s):  
A Vogel ◽  
R Ross ◽  
E Raines
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Blood Serum ◽  
Cell Density ◽  
Whole Blood ◽  
Saturation Density ◽  
Platelet Derived Growth Factor ◽  
3T3 Cells ◽  
Density Dependent ◽  
Dependent Inhibition

The effects of platelet-derived growth factor and plasma components on saturation density in cultures of 3T3 cells were investigated. Both of these components of whole blood serum affect saturation density; however, when 3T3 cells become quiescent at high density in medium containing whole blood serum, only platelet-derived growth factor and fresh whole blood serum are capable of stimulating proliferation. Addition of fresh plasma- derived serum has little effect on cell growth. These results suggest that the platelet factor is the major determinant of saturation density in cultures of 3T3 cells maintained in medium supplemented with whole blood serum. Experiments were performed to investigate the mechanism by which platelet-derived growth factor regulates saturation density. We investigated the possibilities of inactivation of growth factors by proliferating cells, and the effects of cell density on the response of 3T3 cells to platelet-derived growth factor. The amount of platelet- derived growth factor required to initiated DNA synthesis increases with increasing cell density. Some inactivation of growth factors by growing cells was detected, but this depletion was only evident at high cell density. We propose that density-dependent inhibition in cultured 3T3 cells is the result both of an increased requirement for the platelet- derived growth factor as the cultures become more crowded and of inactivation of growth factor activity by growing cells.

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