Large-scale isolation of fraction 1 leaf protein (18S) from lucerne (Medicago sativa L)
1976 ◽
Vol 86
(3)
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pp. 495-501
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Keyword(s):
SummaryFraction 1 protein (18S) can be isolated in large quantities (order 100 g) in a soluble state by heating lucerne juice, adjusted to pH 6·7 to 6·9, from a Pirie extractor to 63 °C for 10 min. Low speed oentrifugation (2500 g) removed coagulated chloroplast fragments and most of the heat-denatured Fraction 2 proteins. Fraction 1 (18S) protein (> 95% pure) was purified from low molecular weight materials (sugars, phenolics etc.) by ammonium sulphate fractionation and gel filtration on Sephadex G-75 gels.
1981 ◽
Vol 46
(03)
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pp. 612-616
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1966 ◽
Vol 44
(5)
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pp. 849-859
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1981 ◽
Vol 153
(4)
◽
pp. 968-976
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1996 ◽
Vol 93
(16)
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pp. 8636-8641
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1981 ◽
1975 ◽
Vol 228
(4)
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pp. 1020-1023
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Keyword(s):
1973 ◽
Vol 30
(10)
◽
pp. 1525-1536
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Keyword(s):