Association of SNP and STR polymorphisms of insulin-like growth factor 2 receptor (IGF2R) gene with milk traits in Holstein-Friesian cows

2018 ◽  
Vol 85 (2) ◽  
pp. 138-141 ◽  
Author(s):  
Marta Dux ◽  
Magdalena Muranowicz ◽  
Eulalia Siadkowska ◽  
Dagmara Robakowska-Hyżorek ◽  
Krzysztof Flisikowski ◽  
...  
Keyword(s):  
Growth Factor ◽  
Biologically Active ◽  
Farm Animals ◽  
Likelihood Method ◽  
Meat Production ◽  
Insulin Like Growth Factor ◽  
Production Traits ◽  
Milk Traits ◽  
Holstein Friesian ◽  
Fat Yield

The objective of the study reported in this Research Communication was to investigate the association of polymorphisms in the insulin-like growth factor receptor 2 (IGF2R) gene with milk traits in 283 Polish Holstein-Friesian (PHF) cows from the IGAB PAS farm in Jastrzębiec. IGF2R regulates the availability of biologically active IGF2 which is considered as a genetic marker for milk or meat production in farm animals. Two novel genetic polymorphisms were identified in the bovine IGF2R gene: a polymorphic TG-repeat in intron 23 (g.72389 (TG)15-67), and a g.72479 G > A SNP RFLP-StyI in exon 24. The following milk traits were investigated: milk yield, protein and fat yield, SCC and lactose content. To determine the influence of the IGF2R STR and SNP genotypes on the milk traits, we used the AI-REML (average information restricted maximum likelihood) method with repeatability, multi-trait animal model based on test-day information using DMU package. Statistical analysis revealed that the G/A genotype (P ≤ 0·01) was associated with milk and protein yield, lactose content and somatic cell count (SCC) in Polish HF cows. TGn (29/22, 28/29, 28/22, 28/28) genotypes were associated with high values for milk, (28/22, 28/23) with protein and fat yield, (25/20) with lactose content, and (29/33, 28/28) with low SCC. We suggest that the IGF2R gene polymorphisms could be useful genetic markers for dairy production traits in cattle.

scholarly journals An association of C/T polymorphism in exon 2 of the bovine insulin-like growth factor 2 gene with meat production traits in Polish Holstein-Friesian cattle

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 227-233 ◽  
Author(s):  
L. Zwierzchowski ◽  
E. Siadkowska ◽  
J. Oprz dek ◽  
K. Flisikowski ◽  
E. Dymnicki
Keyword(s):  
Growth Factor ◽  
Bovine Insulin ◽  
Paternal Allele ◽  
Meat Production ◽  
Insulin Like Growth Factor ◽  
Production Traits ◽  
Exon 2 ◽  
Igf2 Gene ◽  
Holstein Friesian ◽  
Friesian Cattle

The effect of the insulin-like growth factor 2 (IGF2) gene polymorphism – a g.292C>T transition in exon 2 on feed uptake and conversion, growth rates, and meat production traits in Polish Holstein-Friesian cattle was investigated. A total of 291 young bulls were genotyped using restriction fragment length polymorphism (RFLP-BsrI). Animals were slaughtered at the age of 11 or 15 months and carcass traits were examined. At the age of 11 months the association was shown of the CC genotype with higher cold carcass weight, daily gain and meat content (%) in valuable cuts. At 15 months of age the CC genotype bulls showed higher live body weight, whereas those with the CT genotype had more fat in valuable cuts. The TT genotype bulls appeared to consume more feed (dry matter and protein) and used more feed for maintenance and meat production as compared to the CC genotype. The imprinting status of the IGF2 gene was analysed using cDNA sequencing and RFLP-BsrI. In 15-months-old animals and in foetuses older than two months both IGF2 alleles, maternal and paternal, were equally expressed in liver. In a 2-months-old male foetus the IGF2 mRNA was primarily expressed from the paternal allele.  

Association of polymorphisms in exons 2 and 10 of the insulin-like growth factor 2 (IGF2) gene with milk production traits in Polish Holstein-Friesian cattle

2009 ◽  
Vol 77 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Emilia Bagnicka ◽  
Eulalia Siadkowska ◽  
Nina Strzałkowska ◽  
Beata Żelazowska ◽  
Krzysztof Flisikowski ◽  
...  
Keyword(s):  
Growth Factor ◽  
Milk Production ◽  
Insulin Like Growth Factor ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Milk Production Traits ◽  
Milk Traits ◽  
Exon 2 ◽  
Holstein Friesian ◽  
Exon 10

Insulin-like growth factor 2 (IGF2) is considered to be a regulator of post-natal growth and differentiation of the mammary gland. In the present work, associations of two single nucleotide polymorphisms in the bovine IGF2 gene with milk production traits were studied in dairy Holstein-Friesian cows: the already described g.8656C>T transition in exon 2 (RFLP-BsrI) and the newly found g.24507G>T transversion in exon 10 (RFLP-HaeIII), found by sequencing 273-bp exon 10 of the IGF2 gene in six individuals. Associations were analysed individually and in combination with the multi-trait repeatability test-day animal model. The CT/GT haplotype appeared to be associated with most of the milk traits studied (differences were significant at P⩽0·001). The most frequent CT/GG haplotype seemed inferior to others in fat and protein content and daily yield of fat and protein but superior (together with the TT/GG genotype) when the daily milk yield is considered.

scholarly journals The Association of Four Polymorphisms within the Insulin-Like Growth Factor 1 Receptor Gene with Milk Production Traits in Simmental Cows

2016 ◽  
Vol 16 (4) ◽  
pp. 1029-1044 ◽  
Author(s):  
Małgorzata Szewczuk
Keyword(s):  
Growth Factor ◽  
Milk Production ◽  
Milk Fat ◽  
Receptor Gene ◽  
Insulin Like Growth Factor ◽  
Production Traits ◽  
Milk Production Traits ◽  
Gene Encoding ◽  
Milk Traits ◽  
Factor Type

Abstract The aim of this study was to determine the frequency of alleles and genotypes of four polymorphisms located in exons 2, 10 and 16 of the gene encoding insulin-like growth factor type 1 receptor (IGF1R) in the tested herd of 242 Simmental cows and to search for the relationship between these polymorphisms and selected milk production traits. The study applied the following methods: PCR-RFLP and combination of nested PCR and ACRS-PCR. The presence of three genotypes was found for all SNPs. The frequency of alleles was as follows: C - 0.29 and T - 0.71 (IGF1R/e2/MspI), A - 0.33 and G - 0.67 (IGF1R/e2/TaqI), C - 0.77 and T - 0.23 (IGF1R/e10/MspI) and C - 0.53 and T - 0.47 (IGF1R/e16/RsaI). In all lactations, cows with TT (IGF1R/e2/MspI) and GG genotypes (IGF1R/e2/TaqI) produced the highest amounts of milk, fat and protein (P≤0.01), particularly individuals with the combined TT/GG genotypes. As regards the IGF1R/e10/MspI and IGF1R/e16/ RsaI genotypes, the highest milk, fat and protein yields were observed in cows with separate and combined CC/CC genotypes, while the lowest in animals with combined TT/TT genotypes. Cumulative analysis of all genotype combinations showed that individuals with a potentially best combination of TT/GG/CC/CC might be characterized by the highest milk yield as well as fat and protein content in milk. Potentially unfavorable combinations (such as CC/AA/TT/TT and similar) have been almost completely eliminated from the herd tested. The IGF1R gene is proposed as a candidate gene for milk traits in cattle.

scholarly journals Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss

2018 ◽  
Vol 69 (3) ◽  
pp. 1077 ◽  
Author(s):  
S. ARDICLI ◽  
H. SAMLI ◽  
D. DINCEL ◽  
B. EKIZ ◽  
H. YALCINTAN ◽  
...  
Keyword(s):  
Muscle Metabolism ◽  
Bovine Insulin ◽  
Cooking Loss ◽  
Farm Animals ◽  
Meat Production ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Colour Parameters ◽  
Meat Colour ◽  
Meat Samples

Bovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle.

scholarly journals The effect of the farm size on milk traits of cows

2011 ◽  
Vol 27 (3) ◽  
pp. 951-958 ◽  
Author(s):  
D. Kucevic ◽  
S. Trivunovic ◽  
M. Radinovic ◽  
M. Plavsic ◽  
Z. Skalicki ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Dairy Farm ◽  
Farm Size ◽  
Small Farms ◽  
Milk Traits ◽  
Holstein Friesian ◽  
Milk Performance ◽  
Fat Yield ◽  
Large Farms

Objective of this study was to analyze the effect of the dairy farm on milk traits of cows in Vojvodina. The research was carried out on small farms with 10 to 20 cows, medium farms with 20 to 50 cows, and large farms with over 50 dairy cows. The study included registered animals of Simmental (SM) and Holstein-Friesian breed (HF; including Red Holstein) in the first lactation for traits of milk yield and yield and content of milk fat. Total of 1323 first lactations were analyzed. The average milk yield (both breeds) in the first lactation of 305 days was 6295 kg of milk with 234,3 kg of milk fat and average milk fat content of 3,74%. Milk performance of cows varied significantly (CV=22,9% and SD=1447,8), as well as milk fat yield (CV=21,6% and SD=50,8). Large farms produced in average 6534 kg of milk, medium farms 6347kg and small farms 4717kg. Size of the farm exhibited significantly high effect on all observed traits, and the tendency was that farms with higher number of animals realize also higher average of production. Farm management and various breeding-zootechnical conditions present on farms had significant effect on milk performance of cows.

scholarly journals Exploring the genetic variants of insulin-like growth factor II gene and their associations with two production traits in Langshan chicken

2015 ◽  
Vol 45 (1) ◽  
pp. 60-63 ◽  
Author(s):  
Linjun Yan ◽  
Xingtang Fang ◽  
Yao Liu ◽  
Mauricio A. Elzo ◽  
Chunlei Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Genetic Variants ◽  
Insulin Like Growth Factor ◽  
Production Traits ◽  
Factor Ii

Correlation of plasma levels of free insulin-like growth factor 1 and clinical benefit of the IGF-IR inhibitor figitumumab (CP- 751, 871)

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 3539-3539
Author(s):  
M. L. Hixon ◽  
A. Gualberto ◽  
L. Demers ◽  
L. G. Paz-Ares ◽  
S. Novello ◽  
...  
Keyword(s):  
Growth Factor ◽  
Plasma Levels ◽  
Fold Increase ◽  
Serum Markers ◽  
Biologically Active ◽  
Type I ◽  
Insulin Like Growth Factor ◽  
Survival Times ◽  
Significant Treatment ◽  
Treatment Naïve

3539 Background: Free IGF-1 (fIGF-1) represents the biologically active fraction of IGF-1, the main circulating ligand of the Insulin-like Growth Factor type I Receptor (IGF-IR). Signaling through the IGF-IR induces tumor survival and resistance to cancer therapy. Figitumumab (F) (CP-751,871) inhibits IGF-1-induced IGF-IR autophosphorylation with an IC50 of 0.42 nmol/L. Methods: Plasma levels of fIGF-1 were measured in a phase 1b/2 multi-center study of Paclitaxel (T) and Carboplatin (C) and F in patients (pts) with treatment-naïve NSCLC. Other serum markers of the IGF-IR pathway, including circulating soluble IGF-IR (sIGF-IR), IGFBP3 and ALS (acid-labile subunit), were also investigated. The Kaplan-Meier method was used to estimate median survival times. Results: A total of 536 blood samples from 159 pts were analyzed. Baseline median and range fIGF-1 were 0.53 and 0.07–1.99 ng/mL. fIGF-1 directly correlated with IGFBP3 and ALS, and inversely with sIGF-IR (Rho=-0.430, p=0.03). Treatment with F resulted in dose-dependent accumulation of fIGF-1 with a sustained >10 fold increase in fIGF-1 plasma levels at the 20 mg/kg dose, suggesting complete systemic blockade of fIGF-1 binding to the IGF-IR. sIGF-IR decreased and IGFBP3 increased in response to F, but increases in IGBP3 were more modest than those of fIGF-1. Baseline plasma levels of fIGF-1 had a 96.6% negative predictive value for PFS status at 6 months (p=0.03). Median PFS were respectively 2.73 and 6.53 months for TC alone and TC with F (20 mg/kg) in the high fIGF-1 group (p=0.001) while no significant treatment effect of F was observed in the low (<0.54 ng/mL) fIGF-1 group. Sixty three percent of pts with high fIGF-1 had tumors of adenocarcinoma histology. Conclusions: IGF-1 is a key element in the biology of NSCLC of adenocarcinoma histology and its determination may contribute to the identification of pts who benefit from figitumumab therapy. [Table: see text]

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