Comparative whey proteome analysis of small-tailed Han and DairyMeade ovine milk

Abstract We characterized the proteome profile of mid-lactation small-tailed Han (STH) and DairyMeade (DM) ovine milk in order to explore physiological variation and differences in milk traits between the two breeds. Methodology combined a tandem mass tag (TMT) proteomic approach with LC-MS/MS technology. A total of 656 proteins were identified in STH and DM ovine milk, of which 17and 29 proteins were significantly upregulated (P < 0.05) in STH and DM, respectively. Immune-related proteins and disease-related proteins were highly expressed in STH milk, whereas S100A2 and AEBP1 were highly expressed in DM milk, which had beneficial effects on mammary gland development and milk yield. Our results provide a theoretical basis for future breeding of dairy sheep.

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Comparative proteome analysis of rat brain and coronary microvascular endothelial cells

Physiological Research ◽

10.33549/physiolres.930919 ◽

2007 ◽

pp. 159-168

Author(s):

L Lu ◽

PY Yang ◽

Y-Ch Rui ◽

H Kang ◽

J Zhang ◽

...

Keyword(s):

Endothelial Cells ◽

Proteome Analysis ◽

Microvascular Endothelial Cells ◽

Ionization Time ◽

Proteomic Approach ◽

Microvascular Endothelial ◽

Signal Transduction Proteins ◽

Ph Range ◽

Related Proteins ◽

Dimensional Electrophoresis

The endothelium of different organs displays a remarkable heterogeneity, although it presents many common functional and morphological features. However, despite our knowledge of heterogeneity among endothelial cells from different sites, the differences between brain microvascular endothelial cells (BMEC) and coronary microvascular endothelial cells (CMEC) are poorly defined. The aim of this study was to investigate whether BMEC are distinct from CMEC at the protein level. Using the proteomic approach, we comparatively analyzed the proteome of cultured BMEC and CMEC. We reproducibly separated over 2000 polypeptides by using two-dimensional electrophoresis (2-DE) at pH range of 3-10. Using PDQuest software to process the 2-DE gel images, forty-seven protein spots were differentially expressed in the two-endothelial cells. Of these, thirty-five proteins are highly expressed in BMEC, whereas twelve proteins are highly expressed in CMEC. Fifteen proteins in BMEC and seven proteins in CMEC were identified with high confidence by matrix-associated laser desorption ionization time-of-flight mass spectrometer (MALDI-TOF-MS). Our data suggested that BMEC and CMEC were different in several aspects including cytokine and growth-related molecules, stress-related proteins, metabolic enzymes, signal transduction proteins and others. The identification of a set of proteins preferentially expressed in BMEC and CMEC provided new data on the heterogeneity of the endothelium.

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Proteomic profiling of ovine milk after grading up

Journal of Dairy Research ◽

10.1017/s0022029921000455 ◽

2021 ◽

pp. 1-4

Author(s):

Xiaohu Su ◽

Zhong Zheng ◽

Liguo Zhang ◽

Urhan Bai ◽

Ying Ma ◽

...

Keyword(s):

Whey Protein ◽

Protein Profile ◽

Whey Proteins ◽

Female Parent ◽

Proteomic Profiling ◽

Dairy Sheep ◽

Milk Traits ◽

Sheep Milk ◽

Tandem Mass Tag ◽

Milk Industry

Abstract We have previously bred Chinese local dairy sheep through grading up with local Small-Tailed Han (STH) sheep as female parent and DairyMeade (DM) sheep as male parent. In this research communication we characterize the whey protein profile of STH sheep and their offspring (F1, F2) to reveal physiological differences and variation in milk traits. A total of 1032 whey proteins were identified through tandem mass tag labeling (TMT) proteome profiling. Three proteins were significantly differentially abundant between F1 and STH milk, six between F2 and STH milk and five between F1 and F2 milk. In terms of differential changes between generations, WASHC4 and CUTA of F1 and Ig-like domain-containing protein of F2 milk were dominant whey proteins. Overall, the results showed that the whey protein profiles of different generations varied little. The crossbreeds of STH and DM sheep would be suitable for the development of the Chinese local sheep milk industry, and the F2 may be a better population for sheep milk production.

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Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/850512 ◽

2012 ◽

Vol 2012 ◽

pp. 1-14 ◽

Cited By ~ 6

Author(s):

Yu-Dong Xu ◽

Jian-Mei Cui ◽

Yu Wang ◽

Lei-Miao Yin ◽

Chang-Ke Gao ◽

...

Keyword(s):

Lavage Fluid ◽

Specific Protein ◽

Effector Proteins ◽

Acupuncture Points ◽

Two Dimensional Gel Electrophoresis ◽

Drug Candidates ◽

Beneficial Effects ◽

Proteomic Approach ◽

Altered Proteins ◽

Related Proteins

Although the beneficial effects of acupuncture in asthma treatment have been well documented, little is known regarding the biological basis of this treatment. Changes in the lung proteome of acupuncture-treated rats with asthma onset were comparatively analyzed using a two-dimensional gel electrophoresis (2DE) and mass-spectrometry- (MS-) based proteomic approach. Acupuncture on specific acupuncture points appeared to improve respiratory function and reduce the total number of leukocytes and eosinophils in bronchoalveolar lavage fluid in OVA-induced asthma onset. Image analysis of 2DE gels revealed 32 differentially expressed acupuncture-specific protein spots in asthma onset; 30 of which were successfully identified as 28 unique proteins using LC-MS/MS. Bioinformatic analyses indicated that these altered proteins are most likely involved in inflammation-related biological functions, and the functional associations of these proteins result in an inflammation signaling pathway. Acupuncture regulates the pathway at different levels by regulating several key nodal proteins, including downregulating of proinflammatory proteins (e.g., S100A8, RAGE, and S100A11) and upregulating of anti-inflammatory proteins (e.g., CC10, ANXA5, and sRAGE). These deregulated inflammation-related proteins may mediate, at least in part, the antiasthmatic effect of acupuncture. Further functional investigation of these acupuncture-specific effector proteins could identify new drug candidates for the prophylaxis and treatment of asthma.

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