Analysis of T cell populations and IL-3 mRNA expression in mesenteric lymph node cells and intestinal intraepithelial lymphocytes in Strongyloides ratti-infected mice

AbstractT cell populations and IL-3 mRNA expression were analysed in mesenteric lymph node cells and intestinal intraepithelial lymphocytes (IEL) in Strongyloides ratti- infected mice. On days 7 and 12 post-infection, 2.6 times as many mesenteric lymph node cells were present in S. ratti- infected mice compared with uninfected mice. Although the percentages of CD3+, CD4+ and CD8+ cells decreased during infection, the absolute numbers of these cell types increased on day 7 due to an overall increase in the mesenteric lymph node cell number. The CD4/CD8 ratio in IEL was increased on day 5, whereas no significant change in the CD4/CD8 ratio was observed in the mesenteric lymph node cells. Expression of IL-3 mRNA, which is an important cytokine for the induction of murine mucosal mastocytosis and S. ratti- expulsion, was examined in mesenteric lymph nodes and IEL of uninfected and infected mice. IL-3 mRNA was detected in mesenteric lymph nodes of S. ratti-infected mice but not detected in the lymph nodes of uninfected mice. IL-3 mRNA was detected in IEL from both infected and uninfected mice with an 20-fold increase in expression in IEL of infected mice. Overall, IL-3 mRNA levels were higher in IEL than in mesenteric lymph nodes following S. ratti- infection. Expression of IL-4, IL-10, stem cell factor (SCF or c-kit ligand) and IFN-γ mRNA was also examined in these two tissues. IL-10 mRNA was not detected in any tissue examined and IFN-γ mRNA levels were unaltered as a result of an S. ratti- infection. Elevated expression of mRNA for SCF (5-fold) and IL-4 (20-fold) was observed in the mesenteric lymph nodes of infected mice. In contrast, SCF mRNA levels were similar in IEL of uninfected and infected animals and only a modest increase in IL-4 mRNA was observed in IEL of infected mice.

Download Full-text

Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638718825280 ◽

2019 ◽

Vol 31 (2) ◽

pp. 210-216 ◽

Cited By ~ 4

Author(s):

Sandra Felten ◽

Katrin Hartmann ◽

Stefanie Doerfelt ◽

Laura Sangl ◽

Johannes Hirschberger ◽

...

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Mesenteric Lymph Node ◽

Complex Method ◽

Mesenteric Lymph Nodes ◽

Predictive Values ◽

Feline Infectious Peritonitis ◽

Fine Needle ◽

Fine Needle Aspirates ◽

Mesenteric Lymph

Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. However, IHC requires invasive tissue sample collection. We evaluated sensitivity and specificity of an immunocytochemical assay of fine-needle aspirates (FNAs) of mesenteric lymph nodes that can be obtained noninvasively by ultrasound-guided aspiration to diagnose FIP. FNAs of mesenteric lymph nodes were obtained postmortem from 41 cats suspected of having FIP based on clinical and/or laboratory findings. FIP was confirmed immunohistochemically in 30 cats. In the other 11 cats, a disease other than FIP, which explained the clinical signs, was diagnosed histopathologically. Immunocytochemistry (ICC) was performed as an avidin–biotin complex method using a monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, negative and positive predictive values (NPV, PPV, respectively) including 95% confidence intervals (95% CIs) were determined. ICC was positive in 17 of 30 cats with FIP, but also in 1 of 11 control cats that was diagnosed with lymphoma. Sensitivity of ICC was 53% (95% CI: 34–72); specificity 91% (95% CI: 59–100); NPV 42% (95% CI: 22–63); and PPV 94% (95% CI: 71–100). In a lethal disease such as FIP, specificity is most important in order to avoid euthanasia of unaffected cats. Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP, but can be a helpful test in conjunction with other diagnostic measures.

Download Full-text

Influences of orally administered lactoferrin on IFN-γ and IL-10 production by intestinal intraepithelial lymphocytes and mesenteric lymph-node cellsThis paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function, and Applications, and has undergone the Journal's usual peer review process.

Biochemistry and Cell Biology ◽

10.1139/o06-056 ◽

2006 ◽

Vol 84 (3) ◽

pp. 363-368 ◽

Cited By ~ 30

Author(s):

Natsuko Takakura ◽

Hiroyuki Wakabayashi ◽

Koji Yamauchi ◽

Mitsunori Takase

Keyword(s):

Lymph Node ◽

T Cell ◽

T Cell Receptor ◽

Mesenteric Lymph Node ◽

Cell Receptor ◽

Intraepithelial Lymphocytes ◽

Bovine Lactoferrin ◽

Intestinal Intraepithelial Lymphocytes ◽

Mesenteric Lymph ◽

Ifn Γ

Intestinal mucosal immunity plays an important role in mucosal and systemic immune responses. We investigated the influences of orally administered bovine lactoferrin (LF) on cytokine production by intestinal intraepithelial lymphocytes (IEL) and mesenteric lymph-node (MLN) cells, especially T cells. Bovine LF or bovine serum albumin (control) was administered to mice once daily for 3 d. After 24 h from the last administration, IEL of the jejunum and ileum and MLN cells were isolated. These cells were cultured with and without the anti-T-cell-receptor antibody, and then the culture supernatants were assayed for cytokines with ELISA. Oral LF did not affect the ratio of T-cell subpopulations in IEL and MLN; however, LF enhanced both interferon (IFN)-γ and interleukin (IL)-10 production by unstimulated IEL and by IEL stimulated with the αβ T-cell receptor but not with the γδ T-cell receptor. LF also enhanced both IFN-γ and IL-10 production by stimulated and unstimulated MLN cells. The production level of IFN-γ by MLN cells was correlated with that of IL-10. These results suggest that oral LF enhances the production of both Th1-type and Th2/Tr-type cytokines in the small intestine of healthy animals.

Download Full-text

Cavitating Mesenteric Lymph Node Syndrome in Association with Coeliac Disease and Enteropathy Associated T-Cell Lymphoma: A Case Report and Review of the Literature

Case Reports in Medicine ◽

10.1155/2010/478269 ◽

2010 ◽

Vol 2010 ◽

pp. 1-4 ◽

Cited By ~ 6

Author(s):

Olivia M. B. McBride ◽

Richard J. E. Skipworth ◽

Derek Leitch ◽

Satheesh Yalamarthi

Keyword(s):

Case Report ◽

Lymph Node ◽

T Cell ◽

Lymph Nodes ◽

Coeliac Disease ◽

Mesenteric Lymph Node ◽

Cell Lymphoma ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Lymph Node Syndrome

Cavitating mesenteric lymph node syndrome (CMLNS) is a rare and poorly understood complication of coeliac disease (CD), with only 37 cases reported in the literature. CD is an immune-mediated enteropathy, with alterations seen in the small bowel architecture on exposure to ingested gluten. Those who fail to respond to a strict gluten-free diet are termed to have refractory coeliac disease (RCD). This is associated with serious complications such as enteropathy-associated T-cell lymphoma (EATL). We present the case of a 71-year-old female investigated for weight loss and a palpable intraabdominal mass. Abdominal computed tomographic (CT) scan showed multiple necrotic mesenteric lymph nodes. At operation, multiple cavitating mesenteric lymph nodes, containing milky fluid, were found. An incidental EATL was found at the terminal ileum, which was resected. The patient subsequently tested positive for CD. This is the second case report to document an association between CMLNS and EATL. This paper highlights the varied presentation of CD. In this case, the diagnosis of CD was made retrospectively after the complications were dealt with. This paper is followed by a review of relevant literature.

Download Full-text

Transfer of immunity toTrichinella spiralisin the mouse with mesenteric lymph node cells: time of appearance of effective cells in donors and expression of immunity in recipients

Parasitology ◽

10.1017/s0031182000047843 ◽

1977 ◽

Vol 74 (3) ◽

pp. 215-224 ◽

Cited By ~ 64

Author(s):

D. Wakelin ◽

Margaret M. Wilson

Keyword(s):

Lymph Node ◽

Mesenteric Lymph Node ◽

Time Course ◽

Trichinella Spiralis ◽

Defence Mechanism ◽

Spleen Cells ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Lymph Node Cells ◽

Worm Expulsion

Cells capable of transferring immunity toTrichinella spiralis, i.e. of accelerating adult worm expulsion, were present in the mesenteric lymph nodes of mice infected for 4, 6 or 8 days, but not in mice infected for only 2 days. The time-course of worm expulsion in mice infected on the day of transfer was similar in recipients of day 8 cells, expulsion becoming marked only when the recipients had been infected for at least 6 days. Transfer of cells 4 or 6 days after infection did not result in an accelerated worm expulsion; transfer 1 or 2 weeks before infection did not enhance the level of immunity in recipient mice. In contrast to the results obtained with mesenteric lymph node cells (MLNC) no immunity was trsnsferred when recipients were given spleen cells taken from donors infected for 8 days. It is suggested that MLNC do not cause worm expulsion directly, but cooperate with another component of the host's defence mechanism. Accelerated expulsion in recipients of cells was accompanied by a premature decline in fecundity of female worms. Evidence is presented to show that worm expulsion and impaired reproduction may represent independent aspects of the immune response toT. spiralis.

Download Full-text

Different cytokine profiles in mesenteric lymph node cells from HLA-B27 transgenic versus wild type rats stimulated with cecal bacterial antigen

Gastroenterology ◽

10.1016/s0016-5085(01)82562-2 ◽

2001 ◽

Vol 120 (5) ◽

pp. A516-A516

Author(s):

F HOENTJEN ◽

S TONKONOGY ◽

D SPRENGERS ◽

M GOERRES ◽

R SARTOR ◽

...

Keyword(s):

Lymph Node ◽

Mesenteric Lymph Node ◽

Bacterial Antigen ◽

Hla B27 ◽

Wild Type ◽

Cytokine Profiles ◽

Mesenteric Lymph ◽

Lymph Node Cells

Download Full-text

Evidence for the involvement of a bone marrow-derived cell population in the immune expulsion ofTrichinella spiralis

Parasitology ◽

10.1017/s0031182000047855 ◽

1977 ◽

Vol 74 (3) ◽

pp. 225-234 ◽

Cited By ~ 15

Author(s):

D. Wakelin ◽

Margaret M. Wilson

Keyword(s):

Bone Marrow ◽

Lymph Node ◽

Cell Population ◽

Mesenteric Lymph Node ◽

Trichinella Spiralis ◽

Mesenteric Lymph ◽

Lymph Node Cells

When mice were irradiated immediately before infection withTrichinella spiralisthere was a profound and long-lasting interference with their ability to expel adult worms from the intestine. Irradiation given after the fifth day of infection was progressively less effective in this respect. The ability to expel worms was not restored when mesenteric lymph node cells (MLNC) were transferred (a) on the day of infection in mice irradiated one day previously, or (b) on day 7 of an infection in mice irradiated on day 6, even though the MLNC transferred immunity to intact recipients. Transfer of bone marrow (BM) alone was also without effect. However, worm explusion was restored if, following irradiation and injection of BM, 10 days were allowed for BM differentiation before transfer of MLNC. This restoration was effective even after lethal levels of irradiation and was clearly dependent upon a donor-derived BM component cooperating with, or responding to, the activity of the transferred MLNC. The possibility that the BM component is non-lymphoid in nature is discussed.

Download Full-text