Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial serum IgG

1984 ◽  
Vol 58 (3) ◽  
pp. 259-262 ◽  
Author(s):  
M. V. R. Reddy ◽  
Ashok Malhotra ◽  
B. C. Harinath
Keyword(s):  
Immunosorbent Assay ◽  
Sandwich Elisa ◽  
Negative Reaction ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bancroftian Filariasis ◽  
Positive Correlation ◽  
Serum Igg ◽  
Filarial Antigen ◽  
Circulating Antigen

ABSTRACTThe utility of the IgG fraction of human filarial serum immunoglobulin in detecting circulating antigen by sandwich enzyme linked immunosorbent assay (ELISA) was studied. 27 of 33 sera from persons with microfilaracmia, 19 of 30 sera from clinical cases of filariasis, 4 of 30 sera from normal persons from a region endemic for filariasis showed the presence of circulating filarial antigen. All the 20 normal sera from the area where filariasis was not endemic gave negative reaction for filarial antigen. Those sera from persons with microfilaracmia that showed the presence of circulating antigen also showed an apparent positive correlation between the microfilarial density and the antigen titre.

Detection of circulating immune complexes in the sera of dogs infected with Dirofilaria immitis, by Clq-binding enzyme-linked immunosorbent assay

1986 ◽  
Vol 60 (3) ◽  
pp. 239-243 ◽  
Author(s):  
K. Matsumura ◽  
Y. Kazuta ◽  
R. Endo ◽  
K. Tanaka ◽  
T. Inoue
Keyword(s):  
Immunosorbent Assay ◽  
Immune Complexes ◽  
Dirofilaria Immitis ◽  
Significant Positive Correlation ◽  
Age Distribution ◽  
Circulating Immune Complexes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Correlation ◽  
Infection Age

AbstractThe presence of circulating immune complexes (CIC) in the sera of dogs infected with Dirofilaria immitis was detected by using a Clq-binding enzyme-linked immunosorbent assay. Specificity of this assay with different concentrations of heat-aggravated canine IgG (ACG) was observed, i.e., the ELISA readings, expressed as ug equivalents ACG/ml, increased with increasing amounts of ACG. The intra-assay variability was below 10%. The CIC levels of infected and uninfected dogs were 177–0± 104–7 ug/ml and 22–8±45–8 ng/ml (mean±SD), respectively. The highest level was observed in 12 dogs with amicrofilaraemic infection. Age distribution of CIC levels in the 23 infected dogs also showed a significant positive correlation. These findings suggested that the CIC are present in the sera of dogs with dirofilariasis and may relate to canine glomerulonephritis.

scholarly journals Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides

2006 ◽  
Vol 13 (1) ◽  
pp. 150-151 ◽  
Author(s):  
Harry E. Prince
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gliadin Antibodies ◽  
Gliadin Peptides ◽  
Disease Specific ◽  
Inova Diagnostics

ABSTRACT New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.

Enzyme-Linked Immunosorbent Assay For The Detection Of Alloantibodies To Factor IX In Hemophilia B

1981 ◽  
Author(s):  
K H Örstavik ◽  
I Örstavik
Keyword(s):  
Immunosorbent Assay ◽  
Solid Phase ◽  
Factor Ix ◽  
Hemophilia B ◽  
Negative Reaction ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nitrophenyl Phosphate ◽  
Human Factor Ix ◽  
Coagulation Assay

A solid phase enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantitative determination of acquired inhibitors to factor IX. Wells of polystyren Micro-ELISA plates were coated with the IgG fraction of a sheep antiserum to human factor IX. After incubation with pooled normal plasma as a factor IX source, the wells were incubated with test plasma. The binding of alloantibodies to the factor IX-sheep-anti-factor IX complexes was then detected by incubation with alkaline phophatase conjugated antiserum to human IgG. As substrate was used p-nitrophenyl phosphate.Plasma samples from five patients with severe hemophilia B and acquired inhibitors to factor IX were examined. All samples gave a positive reaction in the ELISA. The titers as determined in the ELISA were in good agreement with the titers as determined in a coagulation assay (0.1-800 U/ml). Plasma from 13 patients with hemophilia B and no detectable inhibitor in a coagulation assay all gave a negative reaction in the ELISA. A negative reaction was also found in plasma from four patients with hemophilia A and acquired inhibitors to factor VIII, and in plasma from 15 healthy persons.It is concluded that the ELISA is a simple and sensitive technique for the determination of acquired inhibitors to factor IX in hemophilia B.

Avidin-biotin enzyme-linked immunosorbent assay (AB-ELISA) for the detection of bancroftian filariasis

1993 ◽  
Vol 87 (3) ◽  
pp. 299-302
Author(s):  
P. K. Murthy ◽  
A. M. Khan ◽  
A. Kapil ◽  
A. R. Sircar ◽  
J. C. Katiyar ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bancroftian Filariasis

Sandwich ELISA for Detection of Pig Meat in Raw Beef Using Antisera to Muscle Soluble Proteins

1988 ◽  
Vol 51 (10) ◽  
pp. 790-798 ◽  
Author(s):  
ROSARIO MARTÍN ◽  
JUAN I. AZCONA ◽  
CARMEN CASAS ◽  
PABLO E. HERNÁNDEZ ◽  
BERNABÉ SANZ
Keyword(s):  
Horseradish Peroxidase ◽  
Immunosorbent Assay ◽  
Peroxidase Activity ◽  
Sandwich Elisa ◽  
Soluble Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Double Antibody ◽  
Colored Product ◽  
Pig Meat

A double-antibody sandwich ELISA (enzyme-linked immunosorbent assay) has been successfully developed for the detection of defined amounts of pig meat (1–50%) in raw beef. Antibodies against pig sarcoplasmic extracts were produced in rabbits. Pig-specific antibodies were affinity purified by removing antibodies which crossreacted with horse, chicken or beef extracts followed by immunoadsorption and elution from a pig-extract column. The ELISA involved capturing antigens in sarcoplasmic extracts with pig specific antibodies immobilized on 96-well plates, detecting bound antigen with pig specific, horseradish peroxidase-labeling antibody, and measuring peroxidase activity by the conversion of a clear substrate to a colored product.

Sign in / Sign up

Export Citation Format

Share Document