scholarly journals Evaluation of the INOVA Diagnostics Enzyme-Linked Immunosorbent Assay Kits for Measuring Serum Immunoglobulin G (IgG) and IgA to Deamidated Gliadin Peptides

2006 ◽  
Vol 13 (1) ◽  
pp. 150-151 ◽  
Author(s):  
Harry E. Prince
Keyword(s):  
Celiac Disease ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gliadin Antibodies ◽  
Gliadin Peptides ◽  
Disease Specific ◽  
Inova Diagnostics

ABSTRACT New assays for antibodies to deamidated gliadin peptides (DGP) expressing celiac disease-specific epitopes were evaluated using 154 sera previously tested for endomysial immunoglobulin A (IgA) (EMA), transglutaminase IgA (TGA), and conventional gliadin antibodies. DGP antibody results showed 97% concordance with EMA and TGA results. Of 56 sera negative for EMA and TGA but positive for conventional gliadin antibodies, 54 (96%) were negative for DGP antibodies.

The relation between ischemia modified albumin level and autoimmunity/chronic inflammation in celiac disease

2017 ◽  
Vol 42 (3) ◽  
Author(s):  
Mahmut Yuksel ◽  
Mustafa Kaplan ◽  
Ihsan Ates ◽  
Yasemin Ozderin Ozin ◽  
Hasan Kilic ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Chronic Inflammation ◽  
Immunoglobulin G ◽  
Tissue Transglutaminase ◽  
Immunoglobulin A ◽  
Chronic Inflammatory Disease ◽  
Albumin Level ◽  
Control Group ◽  
Ischemia Modified Albumin ◽  
Gliadin Antibodies

AbstractObjective:We established an expectation that ischemia-modified albumin (IMA) levels are higher in the celiac disease since it is an autoimmune/chronic inflammatory disease. In this study, we determined the level of IMA and its relation to autoimmunity/chronic inflammation in celiac disease.Material and methods:The level of IMA of 65 patients diagnosed with celiac disease and 65 healthy volunteers, was measured with the serum ELISA kit. C-reactive protein (CRP), anti-gliadin antibodies immunoglobulin A (AGA-lgA), anti-gliadin antibodies immunoglobulin G (AGA-lgG), anti-tissue transglutaminase immunoglobulin A antibodies (Anti-t-TGA), anti-tissue transglutaminase immunoglobulin G antibodies (Anti-t-TGG) levels were studied.Results:IMA (30.8 ng/mL vs. 20.1 ng/mL, p=0.006; respectively) levels in celiac patients were higher than the control group. In celiac patients who were antibody positive, IMA level was found to be higher compared to antibody negative patients. A positive correlation was determined between IMA level and AGA-IgA (r=0.504, p<0.001), AGA-IgG (r=0.445, p<0.001), Anti-t TGA (r=0.485, p<0.001), Anti-t TGG (r=0.477, p<0.001) and CRP (r=0.385, p=0.011) levels.Conclusion:Chronic inflammation and autoimmunity were found to be associated with high levels of IMA. To use IMA as a diagnosis and follow-up criterion in celiac disease, IMA levels must be compared before and after treatment of active celiac disease.

scholarly journals Serum Immunoglobulin G Antibody Responses to Bordetella pertussis Lipooligosaccharide and B.parapertussis Lipopolysaccharide in Children with Pertussis and Parapertussis

2001 ◽  
Vol 8 (5) ◽  
pp. 1015-1017 ◽  
Author(s):  
Birger Trollfors ◽  
Teresa Lagergård ◽  
John Taranger ◽  
Elisabet Bergfors ◽  
Rachel Schneerson ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bordetella Pertussis ◽  
Immunoglobulin G ◽  
Antibody Responses ◽  
Serum Immunoglobulin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Bordetella Parapertussis ◽  
The Individual

ABSTRACT Serum immunoglobulin G (IgG) antibodies against the lipooligosaccharide (LOS) of Bordetella pertussis and the lipopolysaccharide (LPS) of Bordetella parapertussiswere measured by enzyme-linked immunosorbent assay in paired sera from 40 children with pertussis and 14 with parapertussis. Wide differences in the individual responses were noted. Both anti-LOS and -LPS IgG levels increased significantly in the children with pertussis, as did anti-LPS but not anti-LOS in those with parapertussis.

scholarly journals Seroprevalence of celiac disease among children in Baghdad, Iraq

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2609-2612
Author(s):  
Huda Sahib Al-Rawazq ◽  
Luay Ibraheem Al-Rawi ◽  
Riyadh Abdul-R Al-Zubaidy
Keyword(s):  
Weight Loss ◽  
Celiac Disease ◽  
Immunoglobulin G ◽  
Chronic Diarrhea ◽  
Serological Test ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Food Ingredient ◽  
Autoimmune Reaction

Celiac disease (CD) represents A unique disorders in which consumption of a food ingredient namely gluten-containing grains (wheat, rye, barley) in combination with genetic susceptibility is fundamental for the development of an a guilefully evolving autoimmune reaction influence the gut and other organs. The present study determines the celiac disease among suspected children. From special the laboratory for Pathogenesis Analyses in Baghdad 100 blood children samples was collected during the period from 1st March 2018 till the 31 th  of July 2018, analyzed by two serological test which were Anti-tissue transglutaminase (tGT) antibodies and Anti-gliadin antibodies for the presence of serum Immunoglobulin A (IgA tGT), Immunoglobulin G (IgG tGT), Immunoglobulin A (IgA AGA), Immunoglobulin G (IgG AGA) were measured by Enzyme-linked Immunosorbent Assay (ELISA). In result obtained positive serological test Trasglutaminase (tGT) for IgA tGT was 5 (16.7 %),  IgG tGT was12 (40.0 %), more than Antiglidin Antibodies IgA AGA was 3 (10.0 %), IgG AGA was 10 (33.3 %). the males was 18 (32.1 %) more than female between (6 year - 9 year) age group which represent 10 (37.0 %). The symptoms of celiac disease found with weight loss 26 (86.7 %), chronic diarrhea 21 (70.0 %), vomiting 19 (63.3 %). In this study, obtained positive serological test Trasglutaminase for (IgA, IgG tGT) Antibodies in male between age group (6 year - 9 year) and with the symptoms more found with weight loss, chronic diarrhea, vomiting.

scholarly journals Seroprevalence of celiac disease among children in Baghdad, Iraq

2021 ◽  
Vol 12 (2) ◽  
pp. 954-957
Author(s):  
Huda Sahib Al-Rawazq ◽  
Luay Ibraheem Al-Rawi ◽  
Riyadh Abdul-R Al-Zubaidy
Keyword(s):  
Weight Loss ◽  
Celiac Disease ◽  
Immunoglobulin G ◽  
Chronic Diarrhea ◽  
Serological Test ◽  
Immunoglobulin A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Food Ingredient ◽  
Autoimmune Reaction

Celiac disease (CD) represents A unique disorders in which consumption of a food ingredient namely gluten-containing grains (wheat, rye, barley) in combination with genetic susceptibility is fundamental for the development of an a guilefully evolving autoimmune reaction influence the gut and other organs. The present study determines the celiac disease among suspected children. From special the laboratory for Pathogenesis Analyses in Baghdad 100 blood children samples was collected during the period from 1st March 2018 till the 31 th of July 2018, analyzed by two serological test which were Anti-tissue transglutaminase (tGT) antibodies and Anti-gliadin antibodies for the presence of serum Immunoglobulin A (IgA tGT), Immunoglobulin G (IgG tGT), Immunoglobulin A (IgA AGA), Immunoglobulin G (IgG AGA) were measured by Enzyme-linked Immunosorbent Assay (ELISA). In result obtained positive serological test Trasglutaminase (tGT) for IgA tGT was 5 (16.7 %),  IgG tGT was12 (40.0 %), more than Antiglidin Antibodies IgA AGA was 3 (10.0 %), IgG AGA was 10 (33.3 %). the males was 18 (32.1 %) more than female between (6 year - 9 year) age group which represent 10 (37.0 %). The symptoms of celiac disease found with weight loss 26 (86.7 %), chronic diarrhea 21 (70.0 %), vomiting 19 (63.3 %). In this study, obtained positive serological test Trasglutaminase for (IgA, IgG tGT) Antibodies in male between age group (6 year - 9 year) and with the symptoms more found with weight loss, chronic diarrhea, vomiting.

scholarly journals Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

1998 ◽  
Vol 5 (5) ◽  
pp. 613-616 ◽  
Author(s):  
Felix Grimm ◽  
Friedrich E. Maly ◽  
Jian Lü ◽  
Roberto Llano
Keyword(s):  
Healthy Volunteers ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Igg Subclass ◽  
Specific Antibodies ◽  
Specific Igg4 ◽  
Antibody Levels

ABSTRACT The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

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