Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Paraná River, Brazil

2015 ◽  
Vol 90 (6) ◽  
pp. 693-697 ◽  
Author(s):  
L.S. Gasques ◽  
R.J. Graça ◽  
S.M.A.P. Prioli ◽  
R.M. Takemoto ◽  
A.J. Prioli
Keyword(s):  
Cryptic Species ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Genetic Data ◽  
Flood Plain ◽  
Fish Parasites ◽  
Coi Gene ◽  
Coi Sequences

AbstractUrocleidoides ectoparasites are mainly found on fish of the neotropical regions. Although molecular research on monogeneans is available, no genetic data exist characterizing species in the Urocleidoides genus. Some DNA sequences have been efficacious in systematic studies and in the reconstruction of phylogenies of fish parasites. Relevant roles have been given to the sequence of the mitochondrial gene of cytochrome c oxidase I (COI). This study characterized COI sequences of the parasites Urocleidoides malabaricusi and U. cuiabai in trahira fish Hoplias aff. malabaricus of the flood plain of the Upper River Paraná, Brazil. The two species under analysis were distinguished by sequencing and analysing a 420-bp fragment of the COI gene, which suggested the existence of the cryptic species U. malabaricusi.

scholarly journals Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences

2016 ◽  
Vol 8 (2) ◽  
pp. 185
Author(s):  
Nia Kurniawaty ◽  
Purnama Hidayat ◽  
Aunu Rauf
Keyword(s):  
Pest Management ◽  
Molecular Characterization ◽  
Dna Sequences ◽  
Biology Education ◽  
Morphological Data ◽  
Coi Gene ◽  
Molecular Characteristics ◽  
Vegetable Crops ◽  
Total Extraction

<p>Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of  Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps  DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis.  PCR programme was succesfully to amplified mt<em>COI</em> gene fragment at 710 bp. The length of mt<em>COI </em>gene of <em>Gynaikothrips uzeli, Haplothrips ganglbaueri</em>, and <em>Pseudophilothrips ichini</em> were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mt<em>COI </em>gene mitochondrial had successfully supported the morphological data. </p><p><strong>How to Cite</strong></p><p>Kurniawaty, N., Hidayat, P. &amp; Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 185-192.</p>

scholarly journals Crenosoma striatum in lungs of European hedgehogs (Erinaceus europeus) from Portugal

2020 ◽  
Vol 57 (2) ◽  
pp. 179-184
Author(s):  
P. F. Barradas ◽  
A. R. Flores ◽  
T. L. Mateus ◽  
F. Carvalho ◽  
F. Gärtner ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
18S Rrna ◽  
Population Sample ◽  
Genetic Data ◽  
Rrna Genes ◽  
12S Rrna ◽  
Rrna Gene ◽  
Mitochondrial 12S Rrna ◽  
18S Rrna Genes

SummaryCrenosoma striatum is a host-specifi c metastrongiloid nematode causing respiratory tract disease in hedgehogs (Erinaceus europaeus). Since few studies have reported C. striatum in hedgehogs and little genetic data is available concerning this lungworm, this study aimed to determine the occurrence of C. striatum in a population sample of hedgehogs from Portugal, additionally providing morphological, histological and molecular data. From 2017 to 2018 a survey of infection was carried out in 11 necropsied hedgehogs. Worms were extracted from fresh lung tissues and microscopically evaluated. Molecular characterization of partial mitochondrial (12S rRNA) and nuclear (18S rRNA) genes was performed. The presence of lungworms in pulmonary tissues of five hedgehogs (45.5%) was detected. Morphological and histopathological analyses evidenced adult forms of nematodes consistent with C. striatum. Molecular characterization of 18S rRNA genes confirmed the classifi cation as C. striatum. Also, novel genetic data characterizing the mitochondrial (12S rRNA) gene of C. striatum is presented.This is the first report of C. striatum infection in hedgehogs of Portugal. The findings here reported provide new insights regarding the geographic distribution and the molecular identification of this lungworm species.

Mitochondrial DNA sequence evidence supporting the recognition of a new North Atlantic Pseudostichopus species (Echinodermata: Holothuroidea)

2004 ◽  
Vol 84 (5) ◽  
pp. 1077-1084 ◽  
Author(s):  
Francisco A. Solís-Marín ◽  
David S.M. Billett ◽  
Joanne Preston ◽  
Alex D. Rogers
Keyword(s):  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Distinct Species ◽  
Molecular Study ◽  
Morphological Characters ◽  
Coi Gene ◽  
Species Status ◽  
Separate Species ◽  
The Family ◽  
A New Species

A new species of the synallactid sea cucumber genus Pseudostichopus is described, P. aemulatus sp. nov., based on genetic (DNA sequences of the mitochondrial gene Cytochrome Oxidase I [COI] gene) and morphological characters. A comparative molecular study with two other species of the same genus (P. villosus and P. mollis) and from a different family (Isostichopus fuscus) was carried out in order to clarify its taxonomic identity. The nucleotide distance between P. aemulatus sp. nov. and P. villosus and P. mollis is sufficient to support distinct species status. The estimated difference in the number of amino acids, coded for by a partially sequenced COI gene, within the species of the family Synallactidae ranged from 4 to 18. The phylogenetic analysis clearly supports separate species status of these sympatric morphotypes, as indicated by the morphological analysis.

Mapping and molecular characterization of five HMG1-related DNA sequences

1998 ◽  
Vol 83 (1-2) ◽  
pp. 124-129 ◽  
Author(s):  
P. Rogalla ◽  
Z. Borda ◽  
K. Meyer-Bolte ◽  
K.H. Tran ◽  
S. Hauke ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Dna Sequences

Molecular characterization of Setaria digitata from Mithun (Bos frontalis)

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Jayanta Kumar Chamuah ◽  
Aseno Sakhrie ◽  
Sanjeevini Lama ◽  
Subhas Chandra ◽  
Gajanan M. Chigure ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Southeast Asia ◽  
Molecular Characterization ◽  
Peritoneal Cavity ◽  
Mitochondrial Gene ◽  
Tribal Population ◽  
Arunachal Pradesh ◽  
Setaria Digitata ◽  
Cultural Life

AbstractMithun is a strongly built hill animal of Southeast Asia and plays an important role in the socio-economic and cultural life of the tribal population. Setaria digitata isolated from peritoneal cavity of mithun both from Arunachal Pradesh and Nagaland were characterized based on conserved region of 12SrDNA, 28SrDNA and ITS-2 and mitochondrial gene CoxI. Based on sequence analysis, it was found to be 99% similarity with Srilankan isolate of S. digitata.

scholarly journals Homology difference analysis of invasive mealybug speciesPhenacoccus solenopsisTinsley in Southern China with COI gene sequence variability

2014 ◽  
Vol 105 (1) ◽  
pp. 32-39 ◽  
Author(s):  
F.Z. Wu ◽  
J. Ma ◽  
X.N. Hu ◽  
L. Zeng
Keyword(s):  
Dna Sequences ◽  
Gene Sequence ◽  
Southern China ◽  
Geographic Origin ◽  
Mitochondrial Genes ◽  
Coi Gene ◽  
Sequence Variability ◽  
Phenacoccus Solenopsis ◽  
The Usa ◽  
Coi Sequences

AbstractThe mealybug speciesPhenacoccus solenopsis(P. solenopsis) has caused much agricultural damage since its recent invasion in China. However, the source of this invasion remains unclear. This study uses molecular methods to clarify the relationships among different population ofP. solenopsisfrom China, USA, Pakistan, India, and Vietnam to determine the geographic origin of the introduction of this species into China.P. solenopsissamples were collected from 25 different locations in three provinces of Southern China. Samples from the USA, Pakistan, and Vietnam were also obtained. Parts of the mitochondrial genes for cytochrome oxidase I (COI) were sequenced for each sample. Homologous DNA sequences of the samples from the USA and India were downloaded from Gen Bank. Two haplotypes were found in China. The first was from most samples from the Guangdong, Guangxi, and Hainan populations in the China and Pakistan groups, and the second from a few samples from the Guangdong, Guangxi, Hainan populations in the China, Pakistan, India, and Vietnam groups. As shown in the maximum likelihood of trees constructed using the COI sequences, these samples belonged to two clades. Phylogenetic analysis suggested that mostP. solenopsismealybugs in Southern China are probably closely related to populations in Pakistan. The variation, relationship, expansion, and probable geographic origin ofP. solenopsismealybugs in Southern China are also discussed.

scholarly journals Intraspecific mitochondrial gene variation can be as low as that of nuclear rRNA

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 339 ◽  
Author(s):  
Tshifhiwa G. Matumba ◽  
Jody Oliver ◽  
Nigel P. Barker ◽  
Christopher D. McQuaid ◽  
Peter R. Teske
Keyword(s):  
Dna Barcoding ◽  
Dna Sequences ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Population Expansion ◽  
Species Level ◽  
Molecular Dating ◽  
Coi Gene ◽  
28S Rrna ◽  
Mtdna Sequence

Background: Mitochondrial DNA (mtDNA) has long been used to date historical demographic events. The idea that it is useful for molecular dating rests on the premise that its evolution is neutral. Even though this idea has long been challenged, the evidence against clock-like evolution of mtDNA is often ignored. Here, we present a particularly clear and simple example to illustrate the implications of violations of the assumption of selective neutrality. Methods: DNA sequences were generated for the mtDNA COI gene and the nuclear 28S rRNA of two closely related rocky shore snails, and species-level variation was compared. Nuclear rRNA is not usually used to study intraspecific variation in species that are not spatially structured, presumably because this marker is assumed to evolve so slowly that it is more suitable for phylogenetics.  Results: Even though high inter-specific divergence reflected the faster evolutionary rate of COI, intraspecific genetic variation was similar for both markers. As a result, estimates of population expansion times based on mismatch distributions differed between the two markers by millions of years. Conclusions: Assuming that 28S evolution is more clock-like, these findings can be explained by variation-reducing purifying selection in mtDNA at the species level, and an elevated divergence rate caused by diversifying selection between the two species. Although these two selective forces together make mtDNA suitable as a marker for species identifications by means of DNA barcoding because they create a ‘barcoding gap’, estimates of demographic change based on this marker can be expected to be highly unreliable. Our study contributes to the growing evidence that the utility of mtDNA sequence data beyond DNA barcoding is limited.

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