Morphological and molecular characterization of Trophurus wuhuensis n. sp. (Nematoda: Telotylenchinae) from soil associated with Cinnamomum camphora in China

AbstractA new plant nematode species, Trophurus wuhuensis n. sp., was collected from the soil associated with Cinnamomum camphora in Wuhu, Anhui Province, China. The new species is characterized by having a female with a slender body 660.5–801.5 μm in length, stylet 12–14 μm long, knobs directed laterad, lateral field marked by short and scattered grooves, post-vulval uterine sac shorter than vulval body diameter, post-rectal intestinal sac absent, tail cylindroid, terminus with deep wrinkles; and male with a pointed tail terminus and spicules 16–18 μm long. The internal transcribed spacer sequences of ribosomal DNA (ITS rDNA) and partial 18S ribosomal DNA (18S rDNA) from T. wuhuensis n. sp. were amplified and sequenced. A phylogenetic analysis based on sequences of 18S rDNA fragments is given in this study.

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A new Cosmocercoides species (Ascaridida: Cosmocercidae), C. wuyiensis n. sp., from the Asiatic frog Amolops wuyiensis (Amphibia: Anura)

Journal of Helminthology ◽

10.1017/s0022149x19000518 ◽

2019 ◽

Vol 94 ◽

Author(s):

Y. Liu ◽

Q. Yu ◽

Y.-L. Shu ◽

J.-H. Zhao ◽

J.-Y. Fang ◽

...

Keyword(s):

18S Rdna ◽

Phylogenetic Analyses ◽

Nematode Species ◽

Coi Gene ◽

Male Body ◽

Lizard Species ◽

Nucleotide Divergence ◽

Distinguishing Features ◽

Morphological And Molecular Characterization

Abstract We identified and characterized a new cosmocercid nematode species, Cosmocercoides wuyiensis n. sp., through microscopic examination and sequencing of the partial small ribosomal RNA gene (18S rDNA), internal transcribed spacer (ITS) and mitochondrial cytochrome c oxidase subunit 1 (COI) genes. The new species was isolated from the intestine of the Asiatic frog Amolops wuyiensis Liu and Hu, 1975 captured from four localities of the Anhui province in south-east China. Among the 25 recorded species of the Cosmocercoides genus, the morphology of C. wuyiensis n. sp. is closest to that of C. kiliwai and C. malayensis, which were isolated from various Mexican frog and Malaysian lizard species, respectively. However, C. wuyiensis n. sp. displayed several distinguishing features, such as small size of the male body, two spicules of unequal lengths in the male, small gubernaculum, pre-, ad- and post-cloacal caudal rosette papillae in the ratio of 18–24:2:6 and simple papillae in the ratio of 14:multiple:4, circle and number of punctation in each rosette at 1:11–16, sharply conical tail-end and the presence of lateral alae and somatic papillae in both sexes. BLAST and the phylogenetic analyses of the 18S rDNA and ITS sequences indicated that C. wuyiensis n. sp. belonged to the genus Cosmocercoides, while that of the COI gene sequence of C. wuyiensis n. sp. showed 16.36% nucleotide divergence with C. pulcher and 47.99% nucleotide divergence with C. qingtianensis. The morphological and molecular characterization of C. wuyiensis n. sp. provides new taxonomic data for this genus.

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Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

Journal of Helminthology ◽

10.1017/s0022149x10000556 ◽

2010 ◽

Vol 85 (3) ◽

pp. 276-282 ◽

Cited By ~ 3

Author(s):

M. Pedram ◽

G. Niknam ◽

M.T. Vinciguerra ◽

W. Ye ◽

R.T. Robbins

Keyword(s):

New Species ◽

Molecular Characterization ◽

Outer Layer ◽

18S Rdna ◽

Soil Samples ◽

28S Rdna ◽

First Report ◽

Morphological And Molecular Characterization

AbstractParactinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

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Morphological and molecular characterization of Pisolithus in soil under eucalyptus plantations in Brazil

Revista Brasileira de Ciência do Solo ◽

10.1590/s0100-06832010000600013 ◽

2010 ◽

Vol 34 (6) ◽

pp. 1891-1898 ◽

Cited By ~ 2

Author(s):

Maria Catarina Megumi Kasuya ◽

Irene da Silva Coelho ◽

Daniela Tiago da Silva Campos ◽

Elza Fernandes de Araújo ◽

Yutaka Tamai ◽

...

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

The Other ◽

Nuclear Ribosomal Dna ◽

Spore Ornamentation ◽

Eucalyptus Plantations ◽

Its Regions ◽

Morphological And Molecular Characterization ◽

Hypogeous Fungus

Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates.

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Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Helminthologia ◽

10.1515/helmin-2015-0068 ◽

2016 ◽

Vol 53 (1) ◽

pp. 62-75 ◽

Cited By ~ 4

Author(s):

Y. Zeng ◽

W. Ye ◽

Z. Zhang ◽

H. Sun ◽

L. Yong ◽

...

Keyword(s):

Acacia Mangium ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

Biodiversity Survey ◽

Close Relationship ◽

Nematode Biodiversity ◽

Morphological And Molecular Characterization ◽

Partial 18S

Summary During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunaniense Wang & Wu, 1992, X. brasiliense Lordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.

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Molecular and morphological characterization of Isthmiophora melis (Schrank, 1788) Luhe, 1909 (Digenea: Echinostomatidae) from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania

Helminthologia ◽

10.1515/helm-2017-0012 ◽

2017 ◽

Vol 54 (2) ◽

pp. 97-104 ◽

Cited By ~ 1

Author(s):

D. Nugaraitė ◽

V. Mažeika ◽

A. Paulauskas

Keyword(s):

18S Rdna ◽

American Mink ◽

Morphological Characterization ◽

Neovison Vison ◽

28S Rdna ◽

Its2 Region ◽

Mustela Putorius ◽

Partial 18S

Summary The specimens collected from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania were morphologically identified as Isthmiophora melis (Schrank, 1788) Lühe, 1909 and were molecularly characterized through sequencing of partial 18S rDNA, 28S rDNA, ITS1- 5.8S-ITS2 region, and ND1. Relations of I. melis to other species of the genus Isthmiophora Lühe 1909 were discussed. According to ITS1 and ND1 sequences the closest species to I. melis is Isthmiophora hortensis (Asada, 1926).

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Morphological and molecular characterization of Ortleppascaris sinensis sp. nov. (Nematoda: Ascaridoidea) from the Chinese alligator Alligator sinensis

Journal of Helminthology ◽

10.1017/s0022149x15000255 ◽

2015 ◽

Vol 90 (3) ◽

pp. 303-311 ◽

Cited By ~ 5

Author(s):

J.H. Zhao ◽

S.S. Wang ◽

G.J. Tu ◽

Y.K. Zhou ◽

X.B. Wu

Keyword(s):

Electron Microscopic Examination ◽

Nematode Species ◽

Small Subunit ◽

Internal Transcribed Spacers ◽

Coi Gene ◽

Electron Microscopic ◽

Chinese Alligator ◽

Alligator Sinensis ◽

Morphological And Molecular Characterization

AbstractA new nematode species, Ortleppascaris sinensis sp. nov. (Ascaridoidea), is described from specimens found in the stomach and intestine of the Chinese alligator Alligator sinensis Fauvel, 1879 (Crocodilian: Alligatoridae) in the National Nature Reserve of Chinese Alligator (Chinese Crocodile Lake) in Anhui Province, China. This is the first description of O. sinensis sp. nov. in both China and this crocodile host, increasing its distribution in South Asia as well as expanding the number of helminths known to infect this crocodile. The detailed description of O. sinensis sp. nov., based on light and scanning electron microscopic examination, provides new taxonomic data for this species, and we also report sequences of the internal transcribed spacers (ITS), small subunit DNA segments (18S) and the cytochrome oxidase I (COI) gene.

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Morphological and molecular characterization of Tarantobelus arachnicida gen. n., sp. n. (Nematoda, Rhabditida, Brevibuccidae), a parasitic nematode of tarantulas

Journal of Helminthology ◽

10.1017/s0022149x17000566 ◽

2017 ◽

Vol 92 (4) ◽

pp. 491-503

Author(s):

J. Abolafia ◽

R. Peña-Santiago

Keyword(s):

New Species ◽

Small Body ◽

Junior Synonym ◽

Lateral Field ◽

Body Diameter ◽

The Family ◽

In Captivity ◽

Basal Bulb ◽

Morphological And Molecular Characterization

AbstractA new genus and new species, Tarantobelus arachnicida, was found in the oral opening of tarantula spiders bred in captivity in Poland. The new species is characterized by having a small body (0.77–0.95 mm long in females and 0.66–0.84 mm in males), cuticle poorly annulated by transverse incisures, lateral field inconspicuous, lips separated with small cuticular flaps topping each lip, stoma panagrolaimoid with gymnostom well developed with robust and refringent rhabdia, pharynx panagrolaimoid with isthmus slightly longer than the basal bulb, intestine with cardiac (anterior) and rectal (posterior) areas with narrower walls. Mature females with intestinal cells including needle crystal packs, excretory pore at isthmus level, female reproductive system panagrolaimoid with post-vulval sac 0.4–0.8 times the length of the corresponding body diameter and having very thick walls, vulva very prominent, female rectum 0.8–1.3 times the length of the anal body diameter, female tail conical with acute tip with phasmids at 58–62% of its length. Male tail conical with long and thin mucro, spicules ventrad bent having rounded manubrium and thick gubernaculum. Description, measurements and illustrations of the new species are provided. Molecular analyses show its relationship with Brevibucca and Cuticonema. On the other hand, Medibulla and its corresponding subfamily Medibullinae, previously in Osstellidae, are transferred to Panagrolaimidae, being Shahnematinae, the junior synonym of Medibullinae. Indocephalobus, recently proposed and located in the family Panagrolaimidae, is considered a junior synonym of Diplogastrellus (Diplogasteromorpha), and its only species, I. zebrae, is considered a junior synonym of D. gracilis. In addition, a key to identification of panagrolaimoid genera is included.

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Morphological and molecular characterization of Aphelenchoides fujianensis n. sp. (Nematoda: Aphelenchoididae) from Pinus massoniana in China

Zootaxa ◽

10.11646/zootaxa.2509.1.3 ◽

2010 ◽

Vol 2509 (1) ◽

pp. 39 ◽

Cited By ~ 18

Author(s):

KAN ZHUO ◽

RUQIANG CUI ◽

WEIMIN YE ◽

MEI LUO ◽

HONGHONG WANG ◽

...

Keyword(s):

Dna Sequences ◽

Pinus Massoniana ◽

Small Subunit ◽

Mt Dna ◽

Lateral Field ◽

Small Subunit Rdna ◽

Aphelenchoides Besseyi ◽

Group 3 ◽

Morphological And Molecular Characterization

Aphelenchoides fujianensis n. sp. is described and illustrated from a dead Pinus massoniana based on morphology and molecular analyses of the near-full-length small subunit rDNA gene and partial cytochrome oxidase subunit I of mitochondrial DNA. This new species belongs to the Group 3 of Aphelenchoides species sensu Shahina with star-shaped tail terminus and is characterised by a relatively long body (653–843 μ m in the male and 803–941 μ m in the female) and four lateral incisures in the lateral field. The male has relatively large spicules (24–30 μ m). The female has elongate postvulval uterine sac (extending ca 32–44% of vulva-anus distance), usually with sperms. Both male and female have star-shaped mucro. It is distinguished from other species by postvulval uterine sac length, a and c ratios, and spicule size and shape. Molecular analysis reveals that this species has unique 18S and mt-DNA sequences, and is closest to Aphelenchoides besseyi in dendrograms inferred using both markers. The identification codes of OEPP/EPPO for A. fujianensis n. sp. are: A1-B2-C1-D1/3-E1-F1/2.

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