scholarly journals Molecular and morphological characterization of Isthmiophora melis (Schrank, 1788) Luhe, 1909 (Digenea: Echinostomatidae) from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania

2017 ◽  
Vol 54 (2) ◽  
pp. 97-104 ◽  
Author(s):  
D. Nugaraitė ◽  
V. Mažeika ◽  
A. Paulauskas
Keyword(s):  
18S Rdna ◽  
American Mink ◽  
Morphological Characterization ◽  
Neovison Vison ◽  
28S Rdna ◽  
Its2 Region ◽  
Mustela Putorius ◽  
Partial 18S

Summary The specimens collected from American mink (Neovison vison) and European polecat (Mustela putorius) in Lithuania were morphologically identified as Isthmiophora melis (Schrank, 1788) Lühe, 1909 and were molecularly characterized through sequencing of partial 18S rDNA, 28S rDNA, ITS1- 5.8S-ITS2 region, and ND1. Relations of I. melis to other species of the genus Isthmiophora Lühe 1909 were discussed. According to ITS1 and ND1 sequences the closest species to I. melis is Isthmiophora hortensis (Asada, 1926).

Characterization of a Tigger1 element from the genome of the American mink (Neovison vison)

Gene ◽  
2012 ◽  
Vol 498 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Amanda Smith ◽  
Katherine Rutherford ◽  
Bernhard Benkel
Keyword(s):  
American Mink ◽  
Neovison Vison

Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

2010 ◽  
Vol 85 (3) ◽  
pp. 276-282 ◽  
Author(s):  
M. Pedram ◽  
G. Niknam ◽  
M.T. Vinciguerra ◽  
W. Ye ◽  
R.T. Robbins
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Outer Layer ◽  
18S Rdna ◽  
Soil Samples ◽  
28S Rdna ◽  
First Report ◽  
Morphological And Molecular Characterization

AbstractParactinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

Morphological and Molecular Characterization of Heterobothrium indicus n. sp. (Monogenea: Diclidophoridae) and Its Phylogenetic Status

2021 ◽  
Author(s):  
A.K. Verma
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Arabian Sea ◽  
28S Rdna ◽  
Coi Gene ◽  
Western Coast ◽  
Monogenean Parasite ◽  
Morphological Studies ◽  
Phylogenetic Status

Background: A new species Heterobothrium indicus n. sp. was isolated from the gills of Upeneus moluccensis (Bleeker, 1855) from the Western Coast of India in Arabian Sea region. The monogenean parasite differs from other congeners by morphological features like the presence of asymmetrical haptoral region, 4th pair of clamp smallest than other three pairs of clamps, genital atrium with 8 hooks, number of testes 29-37 and absence of isthmus.Methods: During the survey of marine fishes at Arabian Sea region, new species of monogenean parasite was isolated from the gills of marine fish Upeneus moluccensis. The parasites were morphologically characterized with the help of light and phase contrast microscopy. 18S rDNA, 28S rDNA, mt COI, ITS1+5.8S and ITS2+5.8S gene regions of parasites were amplified, sequenced and compared with other diclidophorid taxa using different bioinformatics tools.Result: Phylogenetic tree analyses (NJ, ME and MP methods) of 18S rDNA, 28S rDNA and mt COI gene regions are complementing the morphological studies and clearly suggested the placement of this new species under subfamily Choricotylinae, family Diclidophoridae.

scholarly journals Characterization of Colletotrichum Isolates from Tamarillo, Passiflora, and Mango in Colombia and Identification of a Unique Species from the Genus

2003 ◽  
Vol 93 (5) ◽  
pp. 579-587 ◽  
Author(s):  
Lucia Afanador-Kafuri ◽  
Dror Minz ◽  
Marcel Maymon ◽  
Stanley Freeman
Keyword(s):  
Morphological Characterization ◽  
Its2 Region ◽  
Specific Primers ◽  
Molecular Analyses ◽  
Rapd Pcr ◽  
Morphological Criteria ◽  
Polymerase Chain ◽  
Species Specific ◽  
Unique Species

This study was conducted to identify the species of Colletotrichum infecting tamarillo, mango, and passiflora in Colombia and to assess whether cross-infection between host species is occurring. Isolates of Colletotrichum spp. from tamarillo (n = 54), passiflora (n = 26), and mango (n = 15) were characterized by various molecular methods and by morphological criteria. Morphological characterization grouped the tamarillo isolates as C. acutatum and the passiflora and mango isolates as C. gloeosporioides. Species-specific primer analysis was reliable and confirmed grouping of the tamarillo isolates (besides Tom-6) as C. acutatum and the mango isolates (besides Man-76) as C. gloeosporioides. However, DNA of the passiflora isolates was not amplified by either C. acutatum- or C. gloeosporioides-specific primers, but reacted with a new primer, Col1, designed according to the internal transcribed spacer (ITS) 1 region of these isolates. Isolates Tom-6 and Man-76 also reacted positively with the Col1 primer. All the isolates reacting with the C. acutatum- and C. gloeosporioides-specific primers failed to react with primer Col1. Isolate Pass-35 from passiflora did not react with any of the taxon-specific primers. Arbitrarily primed polymerase chain reaction (ap-PCR), random amplified polymerase DNA (RAPD)-PCR, and A+T-rich DNA analyses delineated representative isolates into subgroups within the designated species. Molecular analyses indicated that the C. acutatum tamarillo isolates were uniform or clonal, whereas the C. gloeosporioides mango isolates and Colletotrichum passiflora isolates were heterogeneous. Likewise, sequence analysis of the complete ITS (ITS1-5.8S-ITS2) region identified certain isolates to their respective species: tamarillo isolates as C. acutatum; mango isolates as C. gloeosporioides; passiflora, Tom-6, and Man-76 isolates as a Colletotrichum sp. as yet undefined; and the Pass-35 isolate as an additional undefined Colletot-richum sp. Molecular analyses of the population of Colletotrichum isolates from passiflora, Tom-6 from tamarillo, and Man-76 from mango indicate that this population may not be host specific.

Morphological and molecular characterization of Trophurus wuhuensis n. sp. (Nematoda: Telotylenchinae) from soil associated with Cinnamomum camphora in China

2017 ◽  
Vol 92 (5) ◽  
pp. 637-644
Author(s):  
Y. Li ◽  
J. Du ◽  
Z.Y. Wang ◽  
K. Wang ◽  
H.L. Li
Keyword(s):  
Ribosomal Dna ◽  
18S Rdna ◽  
Nematode Species ◽  
Cinnamomum Camphora ◽  
Lateral Field ◽  
Plant Nematode ◽  
Internal Transcribed Spacer Sequences ◽  
Morphological And Molecular Characterization ◽  
Partial 18S

AbstractA new plant nematode species, Trophurus wuhuensis n. sp., was collected from the soil associated with Cinnamomum camphora in Wuhu, Anhui Province, China. The new species is characterized by having a female with a slender body 660.5–801.5 μm in length, stylet 12–14 μm long, knobs directed laterad, lateral field marked by short and scattered grooves, post-vulval uterine sac shorter than vulval body diameter, post-rectal intestinal sac absent, tail cylindroid, terminus with deep wrinkles; and male with a pointed tail terminus and spicules 16–18 μm long. The internal transcribed spacer sequences of ribosomal DNA (ITS rDNA) and partial 18S ribosomal DNA (18S rDNA) from T. wuhuensis n. sp. were amplified and sequenced. A phylogenetic analysis based on sequences of 18S rDNA fragments is given in this study.

scholarly journals A new species, Dactylosoma piperis n. sp. (Apicomplexa, Dactylosomatidae), from the pepper frog Leptodactylus labyrinthicus (Anura, Leptodactylidae) from Mato Grosso State, Brazil.

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 73
Author(s):  
Letícia Pereira Úngari ◽  
Edward Charles Netherlands ◽  
André Luiz Quagliatto Santos ◽  
Edna Paulino de Alcantara ◽  
Enzo Emmerich ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Peripheral Blood ◽  
18S Rdna ◽  
Mato Grosso ◽  
Lower Vertebrates ◽  
A New Species ◽  
Partial 18S

The Dactylosomatidae Jakowska and Negrelli, 1955 are one of four families belonging to adeleorinid coccidia and comprise the genera Babesiosoma Jakowska and Nigrelli, 1956 and Dactylosoma Labbé, 1894. These blood protozoa occur in peripheral blood of lower vertebrates, and are commonly reported parasitising amphibians. The present study describes Dactylosoma piperis n. sp. from the pepper frog Leptodactylus labyrinthicus (Spix, 1824) (Anura: Leptodactylidae), collected in 2018 at the municipality of Araguaiana, Mato Grosso State, Brazil, based on morphology of intra-erythrocytic trophozoite, primary and secondary merogonic stages and a molecular analysis (partial 18S rDNA). Dactylosoma piperis n. sp. forms a well-supported clade with other Dactylosomatidae. This is the first molecular characterization of a species of Dactylosoma from a Brazilian anuran.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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